Load time: 04/30/2013 02:56:48 PM Read time: 04/30/2013 02:56:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3029 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19176770 cps Min counts: 38602 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18105900.00 19176770.00 18516062.00 98393.00 B 10839460.00 11974031.00 11273620.00 99299.00 C 6792634.00 6822919.00 7219765.00 102205.00 D 3896213.00 4283568.00 4227557.00 102582.00 E 2431314.00 2347165.00 804321.00 587401.00 F 1557282.00 1544247.00 1559008.00 102884.00 G 937808.00 936657.00 918507.00 103582.00 H 541006.00 649324.00 562628.00 77205.00 Load time: 04/30/2013 02:58:26 PM Read time: 04/30/2013 02:58:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3030 Comment: synxnat_pop10 plate Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14143115 cps Min counts: 294606 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7575261.00 10816960.00 3037065.00 3183988.00 2483039.00 3921056.00 3636661.00 5450381.00 5206802.00 1568614.00 1272480.00 589212.00 B 6391728.00 8917741.00 4852775.00 3695972.00 1697490.00 3514662.00 3764878.00 4783533.00 4618161.00 3614673.00 1208312.00 789019.00 C 3604668.00 11896504.00 7135531.00 9052547.00 2540379.00 2793618.00 3579001.00 4739946.00 4663559.00 2708512.00 1875960.00 1302856.00 D 10047459.00 10210959.00 7240992.00 6218716.00 4580489.00 1973375.00 6225815.00 7042288.00 2655771.00 2371172.00 953732.00 784132.00 E 8916383.00 10011015.00 6055151.00 6475209.00 2099165.00 1268310.00 7238137.00 4047470.00 8042764.00 2095505.00 1991788.00 1538681.00 F 9240562.00 14143115.00 10807989.00 8114657.00 2079901.00 2170818.00 5081994.00 9276368.00 9602180.00 3818129.00 1834253.00 1287725.00 G 7072638.00 9230629.00 9798919.00 10122142.00 3187143.00 2159757.00 5808011.00 10296297.00 9475861.00 3532630.00 1604893.00 901073.00 H 4738389.00 2998884.00 5904018.00 6137274.00 7270305.00 2379484.00 7126885.00 10464749.00 5968865.00 2778495.00 1045768.00 722549.00