Load time: 07/10/2013 02:49:36 PM Read time: 07/10/2013 02:49:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3118 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 144019968 cps Min counts: 53342 counts Well list: r0(a8:h12) = cps(f2(a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65423324.00 144019968.00 126631.00 120039.00 117243.00 B 37357904.00 80592680.00 145199.00 126310.00 125195.00 C 23773140.00 48012616.00 128954.00 122249.00 121342.00 D 13789122.00 30312242.00 128312.00 123930.00 122664.00 E 8890972.00 18989746.00 128633.00 124610.00 120700.00 F 5347563.00 11601163.00 111614.00 110141.00 113994.00 G 2880887.00 6075664.00 143763.00 108365.00 108479.00 H 114901.00 143423.00 106684.00 110651.00 108422.00 Load time: 07/10/2013 02:52:06 PM Read time: 07/10/2013 02:52:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3119 Comment: 497 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42696224 cps Min counts: 773689 counts Well list: r0(a8:h12,a1:h5) = cps(f2(a8:h12,a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28013154.00 27046980.00 27658842.00 29814056.00 29005062.00 10346226.00 8333761.00 4419251.00 7283053.00 14857904.00 B 26463866.00 28788456.00 25000960.00 28947870.00 24599686.00 9170818.00 6550120.00 7800415.00 7614494.00 8883938.00 C 26671966.00 28126194.00 26097434.00 29105556.00 29131858.00 9610085.00 12137642.00 7906257.00 8334542.00 8761641.00 D 26708060.00 30028244.00 26066308.00 30646030.00 33269536.00 10787447.00 9939067.00 10281714.00 9693975.00 9259136.00 E 26026760.00 28024520.00 29469290.00 33543012.00 35356552.00 9868780.00 9909888.00 10353147.00 9833085.00 10563687.00 F 23630464.00 27761262.00 26988768.00 31522302.00 38514252.00 11107677.00 8263850.00 9130839.00 9662848.00 8763080.00 G 25300928.00 28345992.00 27887746.00 32176472.00 42696224.00 10352289.00 10838845.00 10199735.00 9111504.00 9651180.00 H 24897660.00 30007746.00 28572418.00 34885932.00 38447652.00 11737955.00 10838572.00 11075351.00 10218785.00 10714707.00 Load time: 07/10/2013 02:54:13 PM Read time: 07/10/2013 02:54:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3120 Comment: 498 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40302216 cps Min counts: 773689 counts Well list: r0(a8:h12,a1:h5) = cps(f2(a8:h12,a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29279702.00 31714706.00 31530784.00 33605080.00 34003108.00 17500530.00 16377471.00 17147226.00 17031606.00 13584776.00 B 28530274.00 27793974.00 28618394.00 30476422.00 30959846.00 16352822.00 16094394.00 16555461.00 16404104.00 15279017.00 C 29569418.00 28964776.00 30634990.00 32815166.00 27799266.00 17070914.00 16181001.00 17020778.00 16800022.00 16934570.00 D 31517936.00 34382852.00 35465952.00 35194428.00 34761168.00 18317082.00 17161452.00 18130236.00 19362478.00 19073824.00 E 31329054.00 34523380.00 36029304.00 37022364.00 36364264.00 18960428.00 18463032.00 20370814.00 16563438.00 19988880.00 F 30654042.00 34391864.00 31379112.00 32933754.00 34513684.00 19374164.00 18240904.00 17017148.00 18003546.00 17270052.00 G 36982904.00 36251300.00 37625880.00 35283348.00 40302216.00 20456886.00 20213654.00 20733902.00 19683406.00 17055976.00 H 29258332.00 30911114.00 34226940.00 34543572.00 36059864.00 20171282.00 21642640.00 22468448.00 20707378.00 18044020.00 Load time: 07/10/2013 02:55:51 PM Read time: 07/10/2013 02:55:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3121 Comment: 499 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29774016 cps Min counts: 773689 counts Well list: r0(a8:h12,a1:h5) = cps(f2(a8:h12,a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29774016.00 13615042.00 12590639.00 13300951.00 14263376.00 18542950.00 18570588.00 17623470.00 15657614.00 14719956.00 B 14022316.00 13031595.00 13686525.00 13719289.00 13049685.00 20042996.00 18746890.00 16430195.00 13109986.00 13434118.00 C 15183993.00 15423390.00 14184300.00 15089994.00 14966982.00 22648268.00 19388240.00 17471404.00 14924800.00 13679556.00 D 15459386.00 17124566.00 16063051.00 15908576.00 18239226.00 22732512.00 23013124.00 21352294.00 18156318.00 17137920.00 E 15503933.00 16273644.00 18423594.00 16254634.00 16871974.00 22641970.00 22974582.00 21312942.00 20301114.00 16968926.00 F 14303588.00 13668536.00 14886694.00 14342940.00 15008675.00 23710560.00 19505652.00 19732652.00 15889528.00 15854654.00 G 13846721.00 13254512.00 11272902.00 15508657.00 14647223.00 22987482.00 18738716.00 18498348.00 14537410.00 15209454.00 H 13971596.00 14208865.00 14707141.00 14318944.00 13804672.00 20569086.00 20231624.00 19546848.00 18688294.00 16064285.00 Load time: 07/10/2013 02:58:23 PM Read time: 07/10/2013 02:58:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3122 Comment: 499 2nd Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25049690 cps Min counts: 773689 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19584204.00 18893474.00 18701710.00 18197956.00 15977566.00 B 20034484.00 20481636.00 18716004.00 15237132.00 14044843.00 C 22724502.00 21137850.00 20753592.00 17406978.00 16441787.00 D 22330318.00 23534002.00 23437048.00 22279520.00 19983772.00 E 21223958.00 23007172.00 22793658.00 25049690.00 17439466.00 F 22086112.00 20211962.00 21321930.00 22345990.00 15129182.00 G 20441644.00 19515886.00 24266452.00 17181650.00 15297717.00 H 20557774.00 20489336.00 19777646.00 22131790.00 16360361.00 Load time: 07/10/2013 02:59:54 PM Read time: 07/10/2013 02:59:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3123 Comment: 500 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25930982 cps Min counts: 56373 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8982062.00 9217153.00 10344399.00 10350461.00 9829412.00 23719428.00 22820686.00 17368646.00 25930982.00 19510126.00 B 8751225.00 8416257.00 8981124.00 8221724.00 112747.00 24016370.00 22094246.00 18068082.00 20612544.00 20735180.00 C 8423350.00 6947732.00 6306002.00 11361449.00 8450887.00 21898078.00 20196610.00 18745072.00 19112274.00 18515702.00 D 8672581.00 7936565.00 7167291.00 8353214.00 8439014.00 22135968.00 21326516.00 18035020.00 21197154.00 22133498.00 E 8355821.00 7050110.00 6522262.00 7772675.00 9077605.00 23180464.00 21598294.00 19676996.00 21090256.00 21880550.00 F 8359893.00 6191603.00 6106190.00 6222978.00 7661569.00 22957676.00 20269578.00 18935230.00 18818202.00 17588254.00 G 7617485.00 6628584.00 6567300.00 6934269.00 7658284.00 23360480.00 19874942.00 20572586.00 19329920.00 20007492.00 H 7152680.00 7168871.00 6531308.00 7009563.00 6339417.00 20425618.00 18436460.00 19200064.00 19082764.00 18611866.00 Load time: 07/10/2013 03:01:56 PM Read time: 07/10/2013 03:01:56 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3124 Comment: 501 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28915736 cps Min counts: 773689 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19945648.00 20161348.00 21946528.00 23130704.00 22686720.00 22394956.00 13476026.00 14346290.00 25884742.00 13083139.00 B 19723046.00 18351884.00 19035438.00 21536790.00 21934394.00 21117080.00 14428506.00 14852998.00 24962886.00 13858850.00 C 20660706.00 18243998.00 18439932.00 21297162.00 20800236.00 22282022.00 13252423.00 13752968.00 25842808.00 12673632.00 D 21742386.00 21131846.00 21863916.00 21355360.00 23341312.00 22466948.00 16701198.00 17364318.00 27263138.00 17145514.00 E 22294414.00 20141404.00 21923518.00 21952318.00 23044928.00 23198744.00 17364238.00 17592852.00 28915736.00 16856720.00 F 21706214.00 19409448.00 19316454.00 19270750.00 22952294.00 23492412.00 15514598.00 14936614.00 25004112.00 16233932.00 G 20724294.00 19508848.00 20630954.00 21515406.00 23164292.00 22225120.00 17330478.00 17697402.00 27885334.00 16203026.00 H 21594976.00 21703958.00 21140334.00 22060038.00 24814528.00 22923934.00 17543030.00 18575492.00 27240102.00 16967102.00 Load time: 07/10/2013 03:03:41 PM Read time: 07/10/2013 03:03:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3125 Comment: 502 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24409158 cps Min counts: 773689 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21108670.00 20726460.00 21530616.00 24409158.00 16992256.00 21079856.00 17455636.00 16572164.00 17109570.00 16046826.00 B 19724000.00 18905710.00 17600538.00 20320630.00 21841328.00 18523326.00 17676134.00 16580972.00 15344562.00 16385829.00 C 20846190.00 16529290.00 14858532.00 19339534.00 20320418.00 18994642.00 17009366.00 15029521.00 14341451.00 15867857.00 D 22057398.00 17267816.00 16763911.00 19825490.00 21404810.00 19181454.00 17870514.00 17098870.00 16499361.00 18169194.00 E 17547882.00 16054085.00 16772887.00 19284868.00 19709328.00 19139270.00 17373948.00 17680698.00 16805824.00 17311942.00 F 18693666.00 12765851.00 13543494.00 15811625.00 19528496.00 18248044.00 17657174.00 15946972.00 14018937.00 16895370.00 G 15730835.00 13579841.00 14590100.00 16486212.00 19660248.00 20156044.00 17831020.00 16642210.00 15553364.00 16945734.00 H 19810614.00 17556486.00 16531478.00 19819140.00 21368278.00 20419418.00 17976396.00 18527186.00 16854222.00 16016562.00 Load time: 07/10/2013 03:05:27 PM Read time: 07/10/2013 03:05:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3126 Comment: 504 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 51289696 cps Min counts: 773689 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36186696.00 33456850.00 38765116.00 40877784.00 46275524.00 16062651.00 16213213.00 15335164.00 11918394.00 11995543.00 B 42793280.00 36120812.00 35851164.00 34341332.00 45460616.00 15383248.00 15415800.00 11592489.00 12406429.00 10877947.00 C 43519748.00 36232964.00 36399676.00 39985620.00 32530386.00 12738905.00 12846910.00 11489270.00 11805460.00 11041558.00 D 43457900.00 44724792.00 41784928.00 40615768.00 40472560.00 13504088.00 12042200.00 12800849.00 16286146.00 12607482.00 E 40504768.00 42863224.00 40164968.00 39815444.00 39984376.00 13130766.00 11707122.00 9567256.00 13360416.00 12339294.00 F 41626884.00 42547596.00 38439052.00 38254692.00 36935584.00 11602817.00 10909192.00 14390104.00 12063963.00 11946606.00 G 40122680.00 38466764.00 40112908.00 39630464.00 42288296.00 12237047.00 11701545.00 14626705.00 15788458.00 14351346.00 H 44699212.00 47972720.00 51092240.00 51289696.00 49135948.00 9833434.00 12468309.00 13216700.00 13199157.00 14130519.00 Load time: 07/10/2013 03:07:11 PM Read time: 07/10/2013 03:07:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3127 Comment: 505 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59170944 cps Min counts: 773689 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23359706.00 26110998.00 25146410.00 26702988.00 26989994.00 35520660.00 32694384.00 27020438.00 34298404.00 27458394.00 B 25072906.00 22753506.00 28539958.00 24425880.00 26540944.00 39734468.00 31531808.00 30571318.00 29584116.00 31544278.00 C 25688358.00 21655726.00 21333398.00 23624762.00 20654806.00 33061682.00 30355296.00 32530522.00 31497410.00 35192508.00 D 26104388.00 24656526.00 22934196.00 25102436.00 24429428.00 36958352.00 35586340.00 37382636.00 37658112.00 16214641.00 E 24542802.00 18473128.00 22221288.00 24545992.00 24813414.00 37745932.00 35478148.00 39841484.00 27171034.00 42575692.00 F 14992273.00 22968646.00 22973152.00 23955764.00 27086940.00 36391292.00 34472160.00 30313314.00 21133116.00 39007468.00 G 16267794.00 21302560.00 19678998.00 27079926.00 28716224.00 39871460.00 45577112.00 34670660.00 36241444.00 36443400.00 H 21971770.00 19443202.00 29639990.00 38281384.00 43505064.00 59170944.00 57792816.00 54050128.00 39537104.00 35025224.00 Load time: 07/10/2013 03:09:21 PM Read time: 07/10/2013 03:09:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3128 Comment: 509 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45885804 cps Min counts: 773689 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24429580.00 23134926.00 40566144.00 45321076.00 45885804.00 43254304.00 35580116.00 30330424.00 28404896.00 28117198.00 B 27371452.00 24610958.00 25466150.00 25783470.00 31921184.00 42461664.00 33736372.00 41338412.00 29440808.00 27655184.00 C 30701726.00 24786626.00 23869514.00 26295226.00 28032540.00 35214772.00 29644646.00 30178518.00 29944218.00 30502132.00 D 30356486.00 27014306.00 27855816.00 27693196.00 29657032.00 37003244.00 33086566.00 31366774.00 29484678.00 29961498.00 E 29075916.00 27962128.00 27762358.00 26225970.00 26819248.00 35841372.00 33102704.00 32557352.00 33277118.00 29964860.00 F 27272172.00 23418530.00 23766714.00 22313574.00 24919388.00 35991256.00 30096812.00 27269384.00 29956856.00 27751902.00 G 27295940.00 22319754.00 24973924.00 26550052.00 27902932.00 33198014.00 34437788.00 29365272.00 29343108.00 28052970.00 H 28080360.00 19154908.00 29627508.00 37442332.00 33816884.00 35090036.00 36403616.00 30471740.00 31433038.00 27900518.00 Load time: 07/10/2013 03:11:10 PM Read time: 07/10/2013 03:11:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3129 Comment: 510 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 62739912 cps Min counts: 773689 counts Well list: r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39908888.00 39599632.00 39031276.00 42854908.00 46611468.00 40282912.00 36614832.00 35308788.00 37799964.00 34213164.00 B 38739880.00 37362056.00 48348656.00 40647984.00 43074172.00 37111336.00 39291544.00 34382388.00 37908496.00 39230576.00 C 40796524.00 39953200.00 38640240.00 40713660.00 40563380.00 37615268.00 35796760.00 34582484.00 42359372.00 42441168.00 D 39553072.00 39233960.00 42911956.00 42026884.00 43005700.00 42685856.00 38785136.00 38830680.00 42261960.00 47267852.00 E 41566496.00 41905104.00 43320904.00 41598908.00 42362388.00 36251296.00 39264820.00 36559124.00 45423784.00 47023948.00 F 40391428.00 43320908.00 41123520.00 38409280.00 42212384.00 37706016.00 36193976.00 37496772.00 43562628.00 55268284.00 G 47670344.00 44713936.00 44043476.00 43281888.00 50549784.00 39050192.00 41770040.00 41387944.00 43717212.00 62739912.00 H 38406044.00 47093496.00 40453304.00 42347548.00 41617476.00 34031672.00 36670192.00 33783216.00 35583392.00 33018370.00