Load time: 7/10/2013 11:53 Read time: 7/10/2013 11:53 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3106 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65309220 cps Min counts: 59859 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 63506168 64784316 65309220 119718 58071640 B 38039760 38563552 38737104 122798 43358584 C 22773502 23502536 24590420 258367 13647386 D 13700302 14972472 14490481 123685 8498441 E 7805886 9333681 8895247 121928 122193 F 4755327 5378423 5421497 121456 122552 G 3023600 3406736 3453921 122722 121645 H 2065288 2072283 2047780 121664 121683 Load time: 7/10/2013 11:54 Read time: 7/10/2013 11:54 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3107 Comment: 482 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31413802 cps Min counts: 773816 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29202708 29597670 27076394 30659544 31127340 6297035 6107314 7867952 7432301 9421124 B 10439568 25879364 25248012 25944962 27956496 13334487 7273604 8678495 8008099 8479667 C 25290840 31413802 23805996 27107980 27550690 8067192 10076940 10084333 11272628 8171091 D 27134504 26047230 25150932 25316162 24945722 7162637 9656758 10800415 14440939 8663173 E 27716376 26453448 25298446 24202634 28382450 10519740 9370857 11263947 11064460 9635846 F 24362574 20256176 23638012 21146854 29759532 7809250 8654656 9352429 10075444 9313068 G 20833044 19598274 21106480 19096108 26584956 6300250 8683422 10102990 8237345 8534076 H 23919686 24701888 23057970 20815220 14865816 9936747 10286322 10156722 9082596 7070605 Load time: 7/10/2013 11:56 Read time: 7/10/2013 11:56 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3108 Comment: 484 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45398432 cps Min counts: 55107 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 32280408 30684954 30715280 29895774 35026040 24726434 25093434 22152962 35129556 110215 B 21855944 30637454 29843086 28855438 37800796 13355960 25673892 21027044 42114976 114428 C 29463114 28807956 28055930 29948654 30373012 24184706 24363496 20346772 39638776 115826 D 33505502 33853340 28620326 32867376 32544874 27480442 24196276 25145784 44349600 117376 E 32763144 30834492 28938636 33799224 29268096 27165938 24997134 24466876 41567940 116034 F 33809116 24554528 24814290 31321872 27782948 29203038 21555010 20524216 39860312 116299 G 30464608 25104750 27333962 32901192 29207668 23377428 21237276 22424128 45398432 116525 H 28741510 28514756 30007532 32348016 28177504 25270936 25681734 27671776 45314156 110537 Load time: 7/10/2013 11:58 Read time: 7/10/2013 11:58 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3109 Comment: 483 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14711169 cps Min counts: 773816 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2979733 3397850 2564026 2567069 3170784 8707538 9759010 8515312 8748708 9555304 B 12762212 3155405 3324825 2475504 3466482 10616103 10596289 10262924 7348117 8860252 C 4377344 4590196 3757876 3398163 3003658 9411969 12041771 11296290 10063428 9614045 D 2896780 4410361 3690271 4144564 3531007 10660109 12135800 13058470 11397469 11172678 E 4261267 4325768 3748982 3695687 3736019 14580966 12917843 12117291 11675259 11489614 F 3061638 4180976 3709861 3322461 3611200 12526813 11395203 12013019 10264054 10140936 G 2763555 14711169 3642127 3266380 2967446 12068496 14137633 11763093 9572479 10620270 H 3174051 3912356 3523009 4280594 3089577 13819283 12349091 10245395 11785183 9404121 Load time: 7/10/2013 11:59 Read time: 7/10/2013 11:59 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3110 Comment: 485 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28946974 cps Min counts: 773816 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21328358 22838566 25048192 28946974 25482564 22323212 26209826 20202286 17506640 16076026 B 8963760 20974668 22876646 19255388 21602186 18213400 19688078 19165062 15475025 14885634 C 20362140 21227838 19525136 23585848 21189822 21509712 20150992 18144174 16157324 16880146 D 20240282 21238032 21488092 24123786 22836544 21818106 20456988 20064102 19799966 18840938 E 20245416 22388252 21603214 23895830 22345128 21423190 20072638 19847296 19009136 19043564 F 22525240 17702282 20136992 21948686 22372132 24203392 18601890 18164018 18462088 17332844 G 21864900 19176038 21873614 24845206 25205742 24620228 16815802 19638560 23641476 13893844 H 19917640 20513878 22384882 24335452 22113460 21329828 20614870 21619508 18793626 20344902 Load time: 7/10/2013 12:01 Read time: 7/10/2013 12:01 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3111 Comment: 486 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26563782 cps Min counts: 773816 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17951934 24314806 10755709 24495842 26563782 7382326 7090840 6359040 5703505 5729345 B 18012190 21076586 13470652 21868670 23395170 6934005 6008049 5857917 4678609 4923260 C 22862924 21829308 17995728 22168384 20991566 6874633 6337245 5571742 4568818 4924581 D 21711652 21304222 17487058 24063694 24894362 5669149 7405854 7090014 7694007 6612746 E 23817718 19291920 16821470 22897832 23830412 6840298 7017284 7236005 6427723 7450356 F 20141186 15956487 15583665 20037968 22792956 5526707 5767251 5318643 5042436 5593162 G 18626248 16564656 14937269 19029516 22449108 5907734 6554310 6173969 5055343 4618291 H 20175844 23135872 19947678 22139464 23548166 6091047 6638854 7076515 5851468 5194391 Load time: 7/10/2013 12:02 Read time: 7/10/2013 12:02 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3112 Comment: 507 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 49082264 cps Min counts: 773816 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20945940 28563696 22558662 21952982 23924494 44621968 49082264 42199264 26937928 28836190 B 8754688 16698055 19181882 21015224 22198910 15642605 23576364 24365104 25946180 22091552 C 20653276 17328616 16622050 20125784 19452054 29410036 37369608 23843482 23761258 30091316 D 20803190 20309764 22195982 23847670 23539284 30147118 27715680 23765344 28093312 39730768 E 25142682 18203032 22338096 12697794 28147372 29610808 27599824 7033568 27219354 31490252 F 23140994 16153682 18152642 19456992 21682146 29031138 21140558 21349304 22747326 25690682 G 18287648 19733364 23079840 17969902 14774140 18630014 24179264 24564580 24526276 28454988 H 17175612 20234034 22161276 18725406 20848760 23982998 31922194 32474674 23720178 26994406 Load time: 7/10/2013 12:04 Read time: 7/10/2013 12:04 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3113 Comment: 508 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 84243232 cps Min counts: 52481 counts Well list: "r0(a1:h5,a8:h12) = cps(f2(a1:h5,a8:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36477712 35262424 34297476 27680980 33002926 122250 120039 120493 117810 113899 B 27839826 33767660 37967344 42766224 33425468 113200 112275 111443 109403 106361 C 30494262 36246016 38324620 8769788 12879572 114391 113446 115449 110839 104963 D 34259364 33118608 29948072 29019378 28176992 112879 113786 113257 110744 106928 E 31074836 35916948 29682708 27663068 30829948 112445 114825 113710 112558 108534 F 34130320 29111632 24012992 42240492 30151584 113181 113427 111765 113011 106702 G 84243232 32194454 35820516 34479952 30047968 112067 110404 107873 108194 106890 H 66495056 51897056 67572768 59185152 29014156 111500 107835 107646 105738 105851