Load time: 07/10/2013 12:11:17 PM Read time: 07/10/2013 12:11:17 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3114 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 154834496 cps Min counts: 53776 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65857820.00 60026240.00 138267888.00 152897888.00 B 36685928.00 39445004.00 39451436.00 154834496.00 C 27058502.00 26968722.00 23954342.00 109649.00 D 16693878.00 16626373.00 14744553.00 110461.00 E 10123901.00 9772159.00 9834968.00 109176.00 F 5751121.00 5949915.00 4908382.00 108799.00 G 3518584.00 3483605.00 3002335.00 107552.00 H 2453277.00 302971.00 359288.00 120153.00 Load time: 07/10/2013 12:13:56 PM Read time: 07/10/2013 12:13:56 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3115 Comment: Samples 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 70745040 cps Min counts: 63883 counts Well list: r0(a1:h5,a6:d6,a7:h8,a9:g9) = cps(f2(a1:h5,a6:d6,a7:h8,a9:g9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23245544.00 31764944.00 44007088.00 28067228.00 33658084.00 12063228.00 2174575.00 129353.00 132357.00 B 26332910.00 40085800.00 26051260.00 48599520.00 38149660.00 2380835.00 834365.00 127766.00 59495496.00 C 36565100.00 67243936.00 22077496.00 21593930.00 19006708.00 12357117.00 4914517.00 136967.00 57997656.00 D 16885636.00 70745040.00 20368464.00 20177316.00 36375172.00 11692741.00 160619.00 167194.00 39080444.00 E 36564064.00 53040352.00 42968784.00 1584528.00 24534832.00 6611113.00 1128419.00 17489744.00 F 12966894.00 37493644.00 12745017.00 20831306.00 9362229.00 5019946.00 137571.00 54174396.00 G 33257102.00 18107192.00 27329812.00 1061541.00 19643396.00 23687940.00 150304.00 25789062.00 H 43933632.00 36286472.00 2924691.00 21752850.00 15660271.00 2008911.00 170556.00 Load time: 07/10/2013 12:15:32 PM Read time: 07/10/2013 12:15:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3116 Comment: Samples 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 74632184 cps Min counts: 58159 counts Well list: r0(a1:h5,a6:d6,a7:h8,a9:g9) = cps(f2(a1:h5,a6:d6,a7:h8,a9:g9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22231564.00 32054958.00 47514780.00 29195436.00 37050048.00 11361672.00 788363.00 116318.00 116960.00 B 26822054.00 38361288.00 28514858.00 45585644.00 35995804.00 2406280.00 722959.00 116847.00 63045156.00 C 38866100.00 74632184.00 28584798.00 20902202.00 19725116.00 12495904.00 4648986.00 126897.00 61795552.00 D 18352142.00 70943512.00 19279904.00 20141186.00 37391644.00 11698221.00 162282.00 150267.00 42765988.00 E 34222700.00 52714436.00 44704712.00 1624651.00 25204922.00 4357805.00 2042546.00 17662564.00 F 13164865.00 39290984.00 14577835.00 21721826.00 9881866.00 3443871.00 1487782.00 54579820.00 G 34303560.00 19594204.00 45828620.00 1065206.00 21624526.00 21737146.00 756379.00 24913676.00 H 43085540.00 35465412.00 2078867.00 22983800.00 15225081.00 1673559.00 452595.00