Load time: 07/22/2013 03:09:14 PM Read time: 07/22/2013 03:09:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3144 Comment: Standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38528224 cps Min counts: 52700 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37844252.00 38528224.00 38489512.00 105401.00 B 26959808.00 26860612.00 26963566.00 106647.00 C 16727129.00 16474644.00 16169610.00 108877.00 D 10508900.00 9814878.00 9889335.00 109236.00 E 6438687.00 5940224.00 6226919.00 108499.00 F 3991360.00 3790058.00 3835385.00 109727.00 G 2408873.00 2341417.00 2327458.00 107347.00 H 1426268.00 1385989.00 1431765.00 110520.00 Load time: 07/22/2013 03:10:17 PM Read time: 07/22/2013 03:10:17 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3145 Comment: T21 Final Libs Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 5018030 cps Min counts: 773835 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3871289.00 4508976.00 3370092.00 3656281.00 B 4752635.00 4427179.00 4197365.00 4109915.00 C 4454393.00 4599423.00 4309668.00 4766670.00 D 5018030.00 4298064.00 4786773.00 4975896.00 E 4225764.00 4986043.00 4294007.00 4415880.00 F 4209150.00 4353181.00 G H Load time: 07/22/2013 03:11:13 PM Read time: 07/22/2013 03:11:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3146 Comment: T21 Final Libs 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 6712106 cps Min counts: 773835 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3894321.00 4529221.00 3372133.00 3643446.00 5297750.00 4128824.00 4555478.00 3527506.00 3756165.00 5552944.00 B 4810130.00 4531257.00 4258807.00 4156631.00 5916277.00 5220424.00 4504813.00 4458027.00 4221764.00 5841617.00 C 4553746.00 4644982.00 4354317.00 4835326.00 6313339.00 4750249.00 4529629.00 4571069.00 4814191.00 6421130.00 D 5066231.00 4349293.00 4836329.00 5018027.00 5368552.00 5432456.00 4177258.00 5058429.00 4847344.00 5277470.00 E 4258256.00 5032732.00 4342511.00 4476266.00 6712106.00 4542612.00 4957516.00 4572601.00 4304464.00 5631720.00 F 4336823.00 4045728.00 4277009.00 4389798.00 5285967.00 4557305.00 4060486.00 4283240.00 4334045.00 5192478.00 G 4028903.00 4980373.00 4982932.00 5404528.00 5622063.00 4312108.00 5158893.00 5016062.00 5104449.00 5717904.00 H 4638714.00 3863694.00 3615803.00 4688343.00 4942989.00 4495625.00 3958357.00 3754979.00 4560472.00 5217056.00