Load time: 07/24/2013 01:36:26 PM Read time: 07/24/2013 01:36:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3148 Comment: Plate513 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14019098 cps Min counts: 773858 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8958924.00 8515515.00 10990373.00 10685863.00 6359966.00 11527038.00 11463008.00 12906654.00 11228978.00 10234382.00 B 10906496.00 10635564.00 10410993.00 10784251.00 10178585.00 13543817.00 13025881.00 13219492.00 11469939.00 11327051.00 C 12758951.00 11972062.00 10344513.00 11682381.00 9510822.00 11984298.00 12192240.00 12182641.00 10111184.00 10489341.00 D 14019098.00 12784962.00 11607454.00 11686969.00 9231933.00 11629204.00 12094637.00 11906248.00 11233216.00 11688734.00 E 12608774.00 12555849.00 9475432.00 8738278.00 6662664.00 10040825.00 10090709.00 10321843.00 8820709.00 10574293.00 F 10744975.00 8752926.00 6918989.00 6240603.00 4424119.00 10311748.00 8242348.00 8475625.00 6339496.00 9041522.00 G 11156791.00 10754966.00 9015000.00 9324337.00 6927778.00 11493569.00 10710844.00 11098134.00 6917572.00 10132499.00 H 12086912.00 11987231.00 10197054.00 11275756.00 7381983.00 11976730.00 11567702.00 10955566.00 12343126.00 10152385.00 Load time: 07/24/2013 01:38:07 PM Read time: 07/24/2013 01:38:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3149 Comment: Plate514 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15354884 cps Min counts: 773858 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12045203.00 11481256.00 15354884.00 13483687.00 9698118.00 8452565.00 7686119.00 9922904.00 8587445.00 6949609.00 B 13567705.00 12926942.00 12177179.00 15068746.00 12408041.00 9832830.00 8692939.00 9238744.00 8277128.00 8431236.00 C 14661103.00 11299909.00 10268850.00 13180786.00 12114462.00 8886661.00 8049329.00 8485411.00 7427327.00 8345916.00 D 13610203.00 11587154.00 10462170.00 12062212.00 10702727.00 8226848.00 8183410.00 8247935.00 7750194.00 9064641.00 E 11817033.00 9286061.00 7261422.00 9495161.00 7222602.00 7146609.00 6873389.00 6993763.00 6743947.00 8322007.00 F 9687678.00 6453021.00 5935275.00 6942520.00 5893495.00 6084314.00 5629441.00 5404251.00 5177769.00 7130574.00 G 8990869.00 7705243.00 8888937.00 9773606.00 8574021.00 8187592.00 6368723.00 7393302.00 6548433.00 6675089.00 H 12965581.00 12439094.00 6864110.00 13294161.00 8843924.00 9001955.00 8715535.00 9140349.00 7062569.00 6716349.00 Load time: 07/24/2013 01:39:52 PM Read time: 07/24/2013 01:39:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3150 Comment: Plate515 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14948848 cps Min counts: 773858 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13155355.00 14724986.00 14948848.00 14727117.00 14357699.00 12273999.00 10985676.00 11229858.00 10663922.00 9958056.00 B 12434199.00 11619375.00 11569813.00 11887956.00 13791564.00 11864337.00 9991910.00 10303029.00 8769888.00 10109929.00 C 12618849.00 10634482.00 9640261.00 12005810.00 12132582.00 10905344.00 10516124.00 10479369.00 10004272.00 9943751.00 D 12453007.00 10468906.00 10678506.00 11483266.00 12467893.00 11017552.00 10933169.00 11431833.00 11586599.00 10872998.00 E 11621155.00 8931555.00 9586746.00 11194330.00 11041666.00 11696666.00 10517825.00 10667979.00 10669362.00 10586024.00 F 10462325.00 7502539.00 8366372.00 8728550.00 10855519.00 10513781.00 9298177.00 9274631.00 9251356.00 9759468.00 G 9506440.00 7602500.00 9097400.00 10492953.00 10234110.00 10595416.00 9055446.00 8916857.00 9154285.00 9178673.00 H 9510927.00 9958543.00 10414201.00 11563622.00 12292716.00 10123260.00 9783034.00 11088576.00 10385829.00 10262286.00 Load time: 07/24/2013 01:41:30 PM Read time: 07/24/2013 01:41:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3151 Comment: Plate516 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21932462 cps Min counts: 773858 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13173426.00 12643693.00 12255223.00 12598840.00 11632037.00 18658970.00 19026624.00 16085189.00 14020166.00 13251387.00 B 12903856.00 12512040.00 11567236.00 12138823.00 10826376.00 18223142.00 17307042.00 14923622.00 13351987.00 13151575.00 C 14066138.00 12111124.00 11497615.00 12202159.00 11579283.00 18734454.00 15130646.00 14062918.00 12983026.00 15580844.00 D 14244297.00 14017372.00 13478661.00 13039599.00 12396711.00 18550682.00 17448698.00 16073096.00 15693329.00 15102182.00 E 13634879.00 12735204.00 12967210.00 12872818.00 11741157.00 18267276.00 18726158.00 15404124.00 15316893.00 14885214.00 F 12461488.00 12132866.00 11233443.00 11539534.00 8469919.00 17177464.00 15983428.00 14461806.00 14564444.00 13718219.00 G 12482776.00 12190778.00 11733431.00 10202713.00 10599784.00 16709205.00 15621448.00 16281954.00 16505247.00 10918546.00 H 12145454.00 10857240.00 14409135.00 12092333.00 10344651.00 17510882.00 14621076.00 21932462.00 17624102.00 15432201.00 Load time: 07/24/2013 01:43:07 PM Read time: 07/24/2013 01:43:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3152 Comment: Plate517 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 28 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27454738 cps Min counts: 773858 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14253427.00 13220507.00 13905063.00 15165964.00 13038434.00 27454738.00 24728496.00 24538890.00 19667284.00 21930598.00 B 14121813.00 12920617.00 12381043.00 13995925.00 13172507.00 26616856.00 24382864.00 19941002.00 17508544.00 19049716.00 C 15033939.00 12571495.00 12374983.00 14074939.00 13977572.00 25217464.00 23671692.00 20593008.00 19165018.00 18253272.00 D 16104103.00 13542465.00 15643871.00 14813326.00 15214685.00 26539356.00 23072566.00 21275338.00 19567824.00 19889496.00 E 15760768.00 13271272.00 12022503.00 14209536.00 13434856.00 25145686.00 22952534.00 21977874.00 19158328.00 19896808.00 F 14821836.00 12138252.00 11302880.00 13507906.00 12960521.00 23562786.00 21695852.00 17762898.00 15855709.00 16613429.00 G 15184383.00 10985442.00 11439675.00 13526795.00 9882745.00 25079676.00 18653576.00 18860750.00 17026250.00 16559816.00 H 15164775.00 14231590.00 13882238.00 13293202.00 11991930.00 23569820.00 21750244.00 20595474.00 19191774.00 18487750.00 Load time: 07/24/2013 01:44:44 PM Read time: 07/24/2013 01:44:44 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3153 Comment: Standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 28.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 139511888 cps Min counts: 1114 counts Well list: r0(a1:h4,a5:g5,h5,a7:h11) = cps(f2(a1:h4,a5:g5,h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 139511888.00 61939896.00 64230208.00 307332.00 309449.00 2493.00 2456.00 2342.00 2286.00 2361.00 B 82095680.00 37772964.00 38515748.00 306199.00 310412.00 2550.00 2437.00 2286.00 2418.00 2418.00 C 50034168.00 23216544.00 23051466.00 306501.00 311961.00 2550.00 2456.00 2418.00 2418.00 2229.00 D 29556324.00 13869027.00 14198821.00 304895.00 316836.00 2474.00 2323.00 2418.00 2550.00 2456.00 E 17587892.00 8578592.00 8437008.00 308069.00 310771.00 2437.00 2493.00 2569.00 2399.00 2323.00 F 10745633.00 5304938.00 5712739.00 310507.00 318007.00 2418.00 2361.00 2361.00 2342.00 2248.00 G 6381007.00 3263967.00 3226787.00 313983.00 312377.00 2342.00 2493.00 2456.00 2474.00 2380.00 H 4023770.00 2329918.00 2175485.00 319235.00 309260.00 2531.00 2493.00 2248.00 2323.00 2342.00