Load time: 08/06/2013 03:59:51 PM Read time: 08/06/2013 03:59:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3170 Comment: Standards,T22 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 58224100 cps Min counts: 119732 counts Well list: r0(a1:h7) = cps(f2(a1:h7)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 58224100.00 248991.00 6237234.00 9061805.00 7714598.00 5101684.00 7194233.00 B 38171036.00 250843.00 5518687.00 9013791.00 7153333.00 5818249.00 6062591.00 C 22783462.00 246855.00 5528524.00 7608914.00 8097940.00 4571950.00 6659421.00 D 13743507.00 248915.00 6091570.00 7301611.00 8163397.00 5377694.00 5528985.00 E 8498621.00 247951.00 6015135.00 6419724.00 8703751.00 3789374.00 5281242.00 F 5339011.00 246175.00 6250402.00 5477941.00 7392630.00 2814006.00 4599573.00 G 3253877.00 250465.00 6446795.00 5593280.00 8828106.00 3311105.00 5471035.00 H 2001892.00 239465.00 8175784.00 3942920.00 5846120.00 4957759.00 4495793.00