Load time: 08/22/2013 02:58:35 PM Read time: 08/22/2013 02:58:35 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3180 Comment: Samples 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 124688104 cps Min counts: 183992 counts Well list: r0(a1:h3) = cps(f2(a1:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 124688104.00 121751144.00 367984.00 B 66472752.00 67291888.00 368985.00 C 38741000.00 37253532.00 373708.00 D 25523874.00 26515620.00 370648.00 E 16856578.00 16301560.00 377165.00 F 10620733.00 10419989.00 375371.00 G 6363662.00 6471240.00 373274.00 H 4011121.00 4144260.00 382134.00 Load time: 08/22/2013 03:00:45 PM Read time: 08/22/2013 03:00:45 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3181 Comment: P21 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21391414 cps Min counts: 773789 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10131662.00 14600572.00 10567660.00 11695234.00 9370554.00 6879736.00 10976374.00 8631399.00 5743707.00 7703927.00 8137288.00 13495959.00 B 6265679.00 10693267.00 12295038.00 10586079.00 6960021.00 21199768.00 16953674.00 11625914.00 9015194.00 6786561.00 6082944.00 8137708.00 C 7140004.00 11352178.00 10182190.00 12326060.00 7735643.00 8505892.00 5229671.00 9879895.00 5883695.00 4510021.00 3213268.00 9251008.00 D 7991216.00 12509609.00 8899410.00 9644916.00 7922489.00 6365813.00 13810271.00 7523482.00 5275167.00 5037649.00 5502933.00 6102594.00 E 13425710.00 8594881.00 13097405.00 12903997.00 6638972.00 7104056.00 11181976.00 11009874.00 5820442.00 4019875.00 5215494.00 6923670.00 F 6392708.00 8838550.00 15467900.00 14990865.00 7661986.00 6241093.00 16463325.00 6024525.00 4859042.00 5877340.00 5350153.00 7955034.00 G 11865716.00 16126358.00 21391414.00 19219472.00 10685131.00 11672670.00 12655860.00 11504675.00 3145829.00 5000746.00 6478391.00 11445387.00 H 10498224.00 11989596.00 13204710.00 9753258.00 10400004.00 9081576.00 13867069.00 9786800.00 5767807.00 6066267.00 9347383.00 16653740.00 Load time: 08/22/2013 03:02:30 PM Read time: 08/22/2013 03:02:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3182 Comment: P22 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25579986 cps Min counts: 773789 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7573392.00 10021570.00 12084418.00 12116151.00 18453522.00 18242436.00 22365794.00 13862449.00 13016503.00 15032043.00 13255062.00 10753857.00 B 5451067.00 10764357.00 15925887.00 10481358.00 18855482.00 14221926.00 18660410.00 17160426.00 11402296.00 14661684.00 13270976.00 11733327.00 C 5568573.00 9149032.00 14115300.00 11699477.00 12587160.00 12048328.00 22966358.00 13419236.00 11463547.00 8942389.00 9321147.00 10224328.00 D 8990620.00 10793909.00 12051068.00 13003465.00 10483119.00 16957130.00 25579986.00 13164207.00 10501600.00 12709616.00 15685820.00 10118466.00 E 12380749.00 8808867.00 18204584.00 10787800.00 11995339.00 17104400.00 14514304.00 13433051.00 15403790.00 10847110.00 11620274.00 13104747.00 F 9281501.00 12882127.00 15484585.00 16349264.00 16031174.00 15763600.00 15887367.00 15753490.00 12355071.00 13417538.00 14906653.00 13690929.00 G 13210571.00 11522765.00 10599972.00 17340440.00 11015408.00 19302222.00 20555178.00 13881200.00 13555277.00 11167453.00 11462273.00 12759832.00 H 14512669.00 12247223.00 13442922.00 19982116.00 11862460.00 16677250.00 15604275.00 12800825.00 11924394.00 11412403.00 8489215.00 13959809.00 Load time: 08/22/2013 03:04:13 PM Read time: 08/22/2013 03:04:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3183 Comment: P23 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22348740 cps Min counts: 773789 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9648692.00 14370549.00 14646864.00 16121117.00 22348740.00 9955746.00 10276116.00 18667162.00 20847302.00 16332389.00 10870959.00 10959833.00 B 12960212.00 9285287.00 8912272.00 14203548.00 17583686.00 10702466.00 8876664.00 18925952.00 15651492.00 11991287.00 10274669.00 12237927.00 C 11828459.00 12160534.00 12874282.00 13134821.00 16454047.00 14372205.00 9553180.00 15411491.00 17004156.00 9884351.00 11267345.00 14425174.00 D 10549766.00 9485178.00 7808010.00 13937304.00 11324780.00 12651433.00 6318959.00 16134394.00 13349667.00 11241497.00 11300007.00 10908383.00 E 14383906.00 7676550.00 16226728.00 17518756.00 12026793.00 9820298.00 12192073.00 15195553.00 16603177.00 11729308.00 12291287.00 14859478.00 F 11169194.00 9733274.00 17383252.00 14323784.00 11733896.00 12193919.00 15142679.00 15943112.00 16376496.00 14168905.00 18043736.00 12483495.00 G 12206652.00 9034530.00 6273939.00 16739992.00 13994068.00 7351039.00 20076212.00 19537670.00 15227309.00 10670471.00 8919454.00 8131149.00 H 11870848.00 11533964.00 12506719.00 15232555.00 15575283.00 9913285.00 18511400.00 12810723.00 21031002.00 11873489.00 15710183.00 14083622.00 Load time: 08/22/2013 03:06:22 PM Read time: 08/22/2013 03:06:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3184 Comment: P25 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26610818 cps Min counts: 504606 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20672310.00 1423330.00 16285882.00 12877002.00 15020400.00 24789900.00 18167568.00 14185426.00 17056632.00 15220091.00 11389859.00 11386824.00 B 13129139.00 1040289.00 17983936.00 11450010.00 18824382.00 22234050.00 15646048.00 14303899.00 19704540.00 14686450.00 8710215.00 9031672.00 C 15837518.00 1009213.00 9418630.00 12882169.00 14441030.00 13546307.00 16813030.00 24176688.00 20222546.00 13402966.00 13491418.00 12879961.00 D 15826924.00 1244750.00 15228687.00 12282509.00 14867156.00 16993550.00 12658511.00 16270025.00 16608845.00 12583333.00 2545439.00 11568814.00 E 13435593.00 1660566.00 13453627.00 13033890.00 15196177.00 18303018.00 16481489.00 3888250.00 26610818.00 14173809.00 12835372.00 13437881.00 F 18146818.00 1384660.00 19028014.00 13887377.00 18063830.00 18626228.00 17974036.00 20516538.00 16547858.00 19079294.00 11332045.00 14585132.00 G 18463388.00 2414708.00 15171598.00 12132506.00 9954900.00 21617972.00 18593376.00 22915416.00 17947896.00 11091019.00 10796077.00 15316618.00 H 16389329.00 3573376.00 14901915.00 15825468.00 18154866.00 19383550.00 16210277.00 17043070.00 13948134.00 10642484.00 7441414.00 18373556.00 Load time: 08/22/2013 03:08:33 PM Read time: 08/22/2013 03:08:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3185 Comment: P26 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13494429 cps Min counts: 218365 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4062439.00 4992727.00 9088927.00 7408072.00 5521290.00 1385680.00 1759424.00 2182975.00 10584253.00 7814002.00 6184566.00 7115285.00 B 6080620.00 6546408.00 4683632.00 5590661.00 7133212.00 5058498.00 5990571.00 9631230.00 10475270.00 11879141.00 10886834.00 2943248.00 C 10191993.00 4887333.00 5491291.00 8517154.00 5448719.00 694804.00 7562098.00 10161998.00 3976554.00 10319930.00 8006297.00 1109791.00 D 3773040.00 4514548.00 10283380.00 6467913.00 6452859.00 874064.00 5708937.00 13494429.00 3617622.00 9875721.00 10827484.00 436730.00 E 5766951.00 8486757.00 4625758.00 11361997.00 7405796.00 1255141.00 8424509.00 6398142.00 5743929.00 5763107.00 6699806.00 6765904.00 F 5233055.00 2203942.00 7077655.00 6941944.00 4955252.00 6152886.00 8553940.00 8722243.00 11142447.00 5449566.00 8338597.00 6240705.00 G 5357873.00 5185578.00 4587985.00 10088519.00 8662320.00 1983687.00 8979092.00 8744806.00 9872798.00 8685754.00 5590714.00 7040190.00 H 8687567.00 6271397.00 4576672.00 5788297.00 4746126.00 2005107.00 8147466.00 11127433.00 12714106.00 10202609.00 2915620.00 6410365.00