Load time: 09/04/2013 12:21:31 PM Read time: 09/04/2013 12:21:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3210 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 103696656 cps Min counts: 79379 counts Well list: r0(a1:h3) = cps(f2(a1:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103696656.00 100302992.00 399827.00 B 60646180.00 59603388.00 404809.00 C 33352854.00 33940392.00 407178.00 D 21778318.00 22894494.00 409499.00 E 13600113.00 13385234.00 420240.00 F 8665915.00 7943459.00 413255.00 G 5196440.00 4880735.00 408235.00 H 3215279.00 3286730.00 423386.00 Load time: 09/04/2013 12:24:37 PM Read time: 09/04/2013 12:24:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3211 Comment: Plate 11 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 23323672 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7850756.00 9590174.00 9140714.00 9894645.00 12312480.00 16379463.00 18929698.00 6018864.00 6135161.00 12253938.00 13330834.00 13413732.00 B 9570749.00 10826633.00 10057623.00 11784235.00 12368692.00 21562976.00 13740183.00 5026136.00 4778978.00 11008917.00 16405868.00 13409427.00 C 9630197.00 7846799.00 12112579.00 13298007.00 11199970.00 22279544.00 13056745.00 6591910.00 11748133.00 6670739.00 16375070.00 19546376.00 D 8830672.00 15499627.00 16016907.00 14802757.00 13757806.00 18849684.00 15002227.00 4415041.00 11235002.00 12988806.00 8994061.00 13440534.00 E 6478872.00 12186403.00 12726830.00 10880502.00 11825138.00 13317639.00 14778506.00 3815400.00 13399241.00 7348823.00 20416612.00 17303120.00 F 10194802.00 4884068.00 9095989.00 14660865.00 10557508.00 9023096.00 13702470.00 4040959.00 14160296.00 14914565.00 23323672.00 14592679.00 G 14073432.00 9097761.00 12030157.00 8262359.00 16352806.00 11677619.00 5179859.00 4275224.00 10436881.00 10795417.00 16888598.00 17448024.00 H 11547326.00 7542094.00 11756311.00 8842082.00 14877666.00 14947987.00 8479569.00 4757212.00 2431174.00 10116715.00 11557246.00 17741274.00 Load time: 09/04/2013 12:27:04 PM Read time: 09/04/2013 12:27:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3212 Comment: Plate 12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 21043366 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17960040.00 13642420.00 13846598.00 15777819.00 9779810.00 15715969.00 8368893.00 10734252.00 10530617.00 12635466.00 9372429.00 8224265.00 B 15943879.00 10157362.00 16175529.00 11237070.00 10374274.00 7765810.00 15481186.00 8939064.00 6110603.00 6958318.00 8557729.00 10040068.00 C 14613632.00 7848738.00 15740901.00 14306789.00 16842372.00 8301501.00 11125876.00 9392279.00 7196064.00 9118978.00 12398054.00 9358727.00 D 18341834.00 9959073.00 13768067.00 9924583.00 14438727.00 14793102.00 8341542.00 9111756.00 10785736.00 6859465.00 12155425.00 10071916.00 E 17779422.00 8157497.00 17478758.00 4414501.00 10861742.00 9848853.00 11743961.00 7679190.00 12155052.00 3954338.00 11523355.00 9072598.00 F 13669437.00 11374418.00 6665026.00 9501783.00 9640958.00 18587502.00 14185347.00 9708404.00 10692320.00 10325960.00 11403503.00 10424682.00 G 16788940.00 11108282.00 13100056.00 9503829.00 9384507.00 15878498.00 13906516.00 9747027.00 8929512.00 14819063.00 6852714.00 9636511.00 H 21043366.00 11546319.00 11844194.00 11522351.00 10394378.00 9573865.00 11886404.00 8476128.00 7256917.00 11782137.00 10668752.00 5599550.00 Load time: 09/04/2013 12:29:32 PM Read time: 09/04/2013 12:29:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3213 Comment: Plate 13 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 29375986 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14223220.00 8712522.00 6485807.00 12082711.00 12199887.00 10631317.00 8196497.00 8458966.00 7937343.00 5686639.00 9524241.00 9617362.00 B 6836778.00 14602342.00 7744067.00 9003344.00 11826195.00 11490827.00 7713965.00 10128590.00 11486005.00 5807030.00 11378819.00 13772843.00 C 15648430.00 15494182.00 9504057.00 9574558.00 8113473.00 7267548.00 9993426.00 9246551.00 7064144.00 7348279.00 8092380.00 14373627.00 D 10884977.00 16247040.00 13964005.00 12268711.00 12215282.00 19187302.00 10538446.00 7599933.00 9055624.00 10231200.00 9840917.00 13488952.00 E 29375986.00 10398055.00 10873645.00 11880890.00 13464242.00 10691600.00 12362720.00 7849282.00 10175852.00 6862549.00 9233429.00 11238502.00 F 10741514.00 10519035.00 9764292.00 17519264.00 11240889.00 14124265.00 6308695.00 7355087.00 6991349.00 5409885.00 8943396.00 8705260.00 G 16746437.00 10200435.00 10552736.00 14563494.00 11833600.00 12984556.00 6266462.00 22848364.00 7985974.00 9222165.00 8121857.00 14242614.00 H 13254042.00 12936732.00 9440871.00 14904202.00 7813048.00 15847115.00 8856583.00 10536068.00 6460154.00 7280279.00 10430847.00 10440725.00 Load time: 09/04/2013 12:31:47 PM Read time: 09/04/2013 12:31:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3214 Comment: Plate 14 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 46680264 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13713360.00 10869476.00 10537327.00 9188114.00 11829191.00 16047343.00 13397884.00 12857108.00 19273022.00 18105878.00 14256939.00 2740986.00 B 13516056.00 8226907.00 14370764.00 10083687.00 14058229.00 12017408.00 13567803.00 13684282.00 14351018.00 39412040.00 22937494.00 2373620.00 C 15082039.00 12759151.00 11345156.00 15534205.00 11569880.00 14674688.00 10620790.00 13000293.00 13968183.00 25218346.00 12271176.00 4914457.00 D 10126523.00 10989560.00 15088918.00 11555731.00 12893656.00 18100922.00 12911271.00 17251600.00 16348763.00 25109062.00 13793186.00 18431698.00 E 10377935.00 10333891.00 10574666.00 9912436.00 16303432.00 16224791.00 12003415.00 13335986.00 5996947.00 17927186.00 16923164.00 12164738.00 F 13063622.00 12851697.00 10616245.00 8758279.00 11619501.00 16257688.00 12201950.00 15393822.00 46680264.00 8802958.00 16445975.00 11772526.00 G 9400510.00 10961334.00 18637692.00 12683518.00 14388477.00 16834158.00 14232401.00 17241858.00 27228686.00 17593040.00 14144149.00 11826371.00 H 12882146.00 8430667.00 9644238.00 11128216.00 10083559.00 11745178.00 12274782.00 17091138.00 44380964.00 9043244.00 6580107.00 11125721.00 Load time: 09/04/2013 12:34:42 PM Read time: 09/04/2013 12:34:42 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3215 Comment: Plate 15 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 21803442 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13447591.00 8668187.00 16557117.00 14503624.00 12864401.00 15422236.00 21803442.00 9205268.00 13157379.00 12897846.00 10904266.00 11901018.00 B 10781342.00 9595622.00 13035304.00 12276871.00 12621603.00 17348876.00 11952447.00 6876162.00 17228760.00 12139438.00 10834760.00 11521683.00 C 9284619.00 9968078.00 14363482.00 13039159.00 12778459.00 14315045.00 11769908.00 15128315.00 18188018.00 9510320.00 9516590.00 10111430.00 D 11930890.00 9938002.00 8138678.00 16721390.00 11720726.00 16839514.00 11180409.00 11769035.00 14759271.00 5753117.00 13315853.00 14098928.00 E 15304486.00 10130271.00 7392005.00 12640898.00 13519738.00 18348618.00 13157597.00 10847938.00 11900483.00 11068630.00 11992897.00 7974421.00 F 13682395.00 5991244.00 9196843.00 11889965.00 17183260.00 8243482.00 20098184.00 11439823.00 13563399.00 8031302.00 15045163.00 6284371.00 G 13159779.00 11219758.00 14885199.00 12622004.00 11162643.00 14025936.00 8777940.00 9719102.00 13245419.00 10413469.00 12079586.00 19109700.00 H 8938863.00 10926781.00 12330650.00 15733101.00 14767233.00 10393970.00 11982578.00 11966862.00 10254197.00 17049290.00 12221488.00 19441536.00