Load time: 09/04/2013 02:12:32 PM Read time: 09/04/2013 02:12:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3217 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 518371 cps Min counts: 218685 counts Well list: r0(a1:h3) = cps(f2(a1:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 440922.00 443095.00 445003.00 B 440355.00 451012.00 457153.00 C 437370.00 447573.00 479713.00 D 456113.00 518371.00 449104.00 E 455207.00 450502.00 479939.00 F 447781.00 452618.00 454375.00 G 441357.00 446345.00 450577.00 H 457512.00 449463.00 460025.00 Load time: 09/04/2013 02:20:22 PM Read time: 09/04/2013 02:20:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3218 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 151653824 cps Min counts: 220707 counts Well list: r0(a1:h3) = cps(f2(a1:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 151653824.00 129567536.00 441414.00 B 79859640.00 84992904.00 448575.00 C 51610136.00 49422696.00 452316.00 D 27603912.00 35027244.00 443719.00 E 17367806.00 18685412.00 453223.00 F 11517949.00 9948387.00 453544.00 G 6855983.00 6466409.00 452731.00 H 4039300.00 4415223.00 454300.00 Load time: 09/04/2013 02:22:19 PM Read time: 09/04/2013 02:22:19 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3219 Comment: Plate 16 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 122641960 cps Min counts: 773919 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23065386.00 31177028.00 50779808.00 38350832.00 30809402.00 37636888.00 32188450.00 47159180.00 28831026.00 33801528.00 37764160.00 27700244.00 B 24740812.00 43707744.00 84804768.00 21495564.00 35510032.00 37091952.00 45149424.00 52550728.00 18269978.00 41797356.00 43860328.00 42583668.00 C 18441546.00 72437272.00 46245036.00 46240332.00 28644602.00 85796624.00 22967502.00 15440043.00 18883096.00 44138236.00 64171924.00 82677560.00 D 29467728.00 57112060.00 59740932.00 56235592.00 39507876.00 41348940.00 24968460.00 15322749.00 26359758.00 30387216.00 51452304.00 86751616.00 E 20693264.00 11747702.00 122641960.00 18187624.00 46164484.00 20881706.00 19670994.00 3203534.00 10423724.00 28448332.00 59235660.00 52860848.00 F 112372376.00 52665160.00 35038500.00 52089504.00 39131128.00 24528628.00 31060910.00 16712552.00 38432536.00 45407172.00 59945456.00 67987296.00 G 76620408.00 69104304.00 34332504.00 57514424.00 41538244.00 20431478.00 39868528.00 26879682.00 50598540.00 49789296.00 94523408.00 95636576.00 H 81851240.00 86567248.00 3875369.00 49219956.00 74548688.00 41220764.00 45220656.00 32899860.00 46945204.00 69687352.00 98209440.00 61983508.00 Load time: 09/04/2013 02:24:29 PM Read time: 09/04/2013 02:24:29 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3220 Comment: Plate 17 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30833092 cps Min counts: 773919 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9297775.00 13315460.00 14063395.00 15673787.00 15041238.00 30833092.00 14345823.00 21717604.00 8248950.00 14102371.00 23703952.00 20536712.00 B 12697702.00 14138957.00 21198870.00 12086058.00 14773213.00 15092452.00 10578254.00 26942286.00 14056599.00 11153769.00 17960712.00 22223876.00 C 18389488.00 12055033.00 15649666.00 11338805.00 14641267.00 20546416.00 13602647.00 22732260.00 14205552.00 18540376.00 27931680.00 19417658.00 D 17310778.00 10275609.00 15441061.00 12506077.00 13036767.00 21438046.00 10894034.00 8315567.00 12037708.00 19305668.00 25244308.00 22468210.00 E 15716150.00 18135756.00 8476158.00 15173471.00 14855620.00 10108857.00 9352652.00 7905490.00 16042949.00 13633198.00 16554577.00 22096716.00 F 15237522.00 13196330.00 11972047.00 16367410.00 14828239.00 23867926.00 19137530.00 6045573.00 14325429.00 16636568.00 30199038.00 21783132.00 G 13612120.00 18389228.00 20205888.00 16610110.00 14528551.00 26452558.00 12351984.00 7455516.00 16325150.00 20127274.00 19843080.00 21866468.00 H 14332247.00 7398483.00 14333043.00 14443707.00 12694973.00 12409184.00 15974205.00 6398178.00 22311398.00 23850564.00 19800032.00 17194780.00 Load time: 09/04/2013 02:26:54 PM Read time: 09/04/2013 02:26:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3221 Comment: Plate 18 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 7904109 cps Min counts: 218042 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7848067.00 5195752.00 4939354.00 1888868.00 467129.00 471248.00 512136.00 463501.00 465995.00 457531.00 449349.00 436085.00 B 7904109.00 6186701.00 6782862.00 3823354.00 468773.00 477502.00 468395.00 474555.00 462934.00 457209.00 456000.00 449633.00 C 2445236.00 5032849.00 3919361.00 4827720.00 469170.00 470643.00 472306.00 467337.00 467110.00 464408.00 469396.00 440166.00 D 2340487.00 3879445.00 3817616.00 2715782.00 470454.00 468603.00 479618.00 468055.00 482793.00 462046.00 540893.00 449519.00 E 3625481.00 2419177.00 2613163.00 1869911.00 474082.00 468792.00 472608.00 470322.00 476368.00 471229.00 460988.00 455603.00 F 6009343.00 3755678.00 2290778.00 1582358.00 469906.00 484040.00 471380.00 465107.00 468282.00 466184.00 463558.00 449557.00 G 4319146.00 2149732.00 2180789.00 1980840.00 474252.00 476387.00 477238.00 468697.00 465825.00 462613.00 466959.00 454677.00 H 5298320.00 2985346.00 1972781.00 5570257.00 471796.00 471078.00 476992.00 491012.00 461933.00 454659.00 457247.00 453997.00 Load time: 09/04/2013 02:29:37 PM Read time: 09/04/2013 02:29:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3222 Comment: Plate 19 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 33 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32647682 cps Min counts: 773919 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22436166.00 13040457.00 14971492.00 17482404.00 15660211.00 18301906.00 10982167.00 15314168.00 12171479.00 11329906.00 6260434.00 15200621.00 B 25715468.00 10682403.00 16187507.00 15866621.00 16511452.00 16898328.00 14395669.00 13687304.00 7015074.00 15988229.00 9385398.00 13646780.00 C 18042276.00 12649119.00 17427880.00 16133548.00 8411595.00 13632382.00 15578316.00 8497050.00 11390471.00 10691243.00 10524610.00 20508360.00 D 18805824.00 16594866.00 17865270.00 17111670.00 12484149.00 12712761.00 10312400.00 14672607.00 10331549.00 7847359.00 11226594.00 18358560.00 E 11697754.00 9102347.00 17154340.00 17538902.00 14999959.00 18023326.00 19619134.00 18794042.00 13542236.00 9304057.00 9158326.00 29924980.00 F 14683380.00 12276122.00 18025930.00 13089021.00 17262782.00 15530766.00 11425937.00 17682524.00 13506032.00 13627965.00 9981001.00 32647682.00 G 12957165.00 10353886.00 14238351.00 17185234.00 15466424.00 14093589.00 21127570.00 16324874.00 14490628.00 11497245.00 16660957.00 29033826.00 H 11641481.00 24400172.00 14476858.00 11417306.00 18639778.00 15169872.00 18631432.00 13921349.00 10799796.00 14682600.00 16532298.00 27026304.00 Load time: 09/04/2013 02:32:12 PM Read time: 09/04/2013 02:32:12 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3223 Comment: Plate 20 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 33.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40529760 cps Min counts: 211958 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20677566.00 17330856.00 22061570.00 17106604.00 7490920.00 10591776.00 9047863.00 15349887.00 18505046.00 16795900.00 19563734.00 435367.00 B 21452900.00 15780145.00 25443828.00 19189062.00 8309049.00 12349502.00 7929246.00 12245702.00 17824698.00 17238442.00 18148046.00 439430.00 C 29125282.00 21670840.00 31395706.00 15226609.00 14005875.00 18356600.00 13602694.00 13187425.00 33667424.00 15761056.00 29141948.00 445306.00 D 24865694.00 40529760.00 18696154.00 15262914.00 11730163.00 11202899.00 6643955.00 7764199.00 25252546.00 22143248.00 14611055.00 461536.00 E 26617502.00 17860978.00 26185132.00 18770748.00 10773952.00 14582450.00 15672136.00 11333490.00 34968364.00 23126316.00 11314469.00 714893.00 F 29638586.00 35633308.00 27832536.00 34883884.00 11633606.00 11443999.00 12426958.00 12937044.00 25346996.00 11835031.00 12409084.00 423917.00 G 24384260.00 28442794.00 27308682.00 14021556.00 12176612.00 12638376.00 10617597.00 15847452.00 27117118.00 21964518.00 13820712.00 466902.00 H 26802792.00 23529620.00 24101092.00 12846998.00 9125595.00 15859827.00 13096531.00 14562886.00 24835930.00 14376976.00 19771652.00 428301.00