Load time: 09/05/2013 02:28:12 PM Read time: 09/05/2013 02:28:12 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3227 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 112316768 cps Min counts: 198267 counts Well list: r0(a1:h3) = cps(f2(a1:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 112316768.00 108659608.00 396534.00 B 63973428.00 66071336.00 410742.00 C 34110740.00 38723504.00 413047.00 D 23161968.00 21309914.00 410194.00 E 15963402.00 15724317.00 668429.00 F 9532449.00 9384190.00 414445.00 G 5821134.00 5745059.00 410837.00 H 3650356.00 3548343.00 409155.00 Load time: 09/05/2013 02:30:50 PM Read time: 09/05/2013 02:30:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3228 Comment: Plate 21 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 20544922 cps Min counts: 210727 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13739901.00 9419818.00 12136417.00 10995274.00 10604905.00 11825465.00 15690808.00 12070787.00 14695667.00 12375426.00 11762055.00 10969791.00 B 13973053.00 10531832.00 10810316.00 14584853.00 13291183.00 15281899.00 14113130.00 15771231.00 15040373.00 19336068.00 9016660.00 10368611.00 C 16818298.00 9595971.00 15012363.00 12957176.00 16587797.00 11235558.00 16235374.00 13754479.00 15147265.00 13532882.00 14667228.00 15873488.00 D 13109328.00 11332152.00 8700666.00 12792910.00 10700185.00 20168194.00 12013932.00 13165104.00 14489875.00 9830051.00 10127356.00 11620948.00 E 16478477.00 5873653.00 14692206.00 14062047.00 13593737.00 15784388.00 13190663.00 11214506.00 20544922.00 12878193.00 11017017.00 424893.00 F 12783970.00 9804719.00 15050171.00 11398937.00 16869002.00 8902233.00 14114264.00 11111325.00 16830478.00 11627426.00 11425864.00 422834.00 G 13514519.00 9640212.00 11816672.00 12374221.00 11697782.00 10269652.00 15108556.00 11268377.00 13947290.00 12238468.00 11085285.00 427047.00 H 10668486.00 11264046.00 11365588.00 13905838.00 11185199.00 16637367.00 15028339.00 13941110.00 15034149.00 14009149.00 11092341.00 421455.00 Load time: 09/05/2013 02:33:10 PM Read time: 09/05/2013 02:33:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3229 Comment: Plate 22 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28652868 cps Min counts: 205172 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2650328.00 4771505.00 10436218.00 8647775.00 7063406.00 16623428.00 28652868.00 13390929.00 7795638.00 411819.00 428181.00 420075.00 B 4471305.00 1656762.00 725960.00 11720513.00 8818391.00 20385970.00 17159978.00 17944650.00 6081250.00 410345.00 423968.00 413916.00 C 1495280.00 1723751.00 13130370.00 10818568.00 8609004.00 20462350.00 24800866.00 11255692.00 9576555.00 416561.00 430013.00 424969.00 D 872988.00 1153272.00 12934155.00 6149273.00 7684851.00 8896829.00 18919592.00 13145581.00 11089703.00 417714.00 431695.00 418073.00 E 1118489.00 5058917.00 9099687.00 562853.00 7149643.00 15049001.00 13878257.00 13022522.00 5030174.00 429692.00 432847.00 428918.00 F 3308162.00 4962460.00 1758161.00 10195657.00 16650681.00 15132278.00 15364455.00 8922000.00 3335387.00 450418.00 426745.00 423042.00 G 2027572.00 808996.00 10254127.00 8668008.00 7536951.00 19939444.00 19368004.00 6925510.00 3777837.00 437798.00 418243.00 429352.00 H 1165402.00 7530519.00 10371725.00 1884161.00 16096817.00 22588128.00 20071006.00 5714624.00 2928951.00 426310.00 433584.00 421455.00 Load time: 09/05/2013 02:35:54 PM Read time: 09/05/2013 02:35:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3230 Comment: Plate 23 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41963712 cps Min counts: 197530 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28775282.00 31348944.00 32784870.00 41267984.00 23502854.00 12460552.00 14571561.00 9780531.00 12419159.00 16642089.00 16747905.00 395061.00 B 30274618.00 25192022.00 35790208.00 26630298.00 25179404.00 22170350.00 23405330.00 17387452.00 14421665.00 17738414.00 22872260.00 419905.00 C 29293540.00 37719396.00 21818068.00 29379728.00 30827450.00 41893520.00 15730548.00 16254575.00 19019284.00 28352738.00 22195148.00 406586.00 D 29970598.00 32952394.00 39526308.00 32416682.00 20876912.00 29045064.00 17414058.00 10178277.00 15996102.00 19303798.00 22650474.00 408569.00 E 19419668.00 37516968.00 39114956.00 30987426.00 29648518.00 26915664.00 20999106.00 15443160.00 20999906.00 22271288.00 16876394.00 422569.00 F 26472792.00 35160896.00 31743198.00 38623392.00 22778076.00 24708928.00 14223765.00 14454339.00 17714862.00 21011994.00 19694704.00 430448.00 G 31859244.00 41733336.00 28576156.00 24081888.00 40238864.00 14777523.00 12277846.00 12215074.00 17940114.00 27090446.00 18185134.00 427368.00 H 33343552.00 41963712.00 31201316.00 28648304.00 41247972.00 22527112.00 13221809.00 8545417.00 14627419.00 21190236.00 15940975.00 399501.00 Load time: 09/05/2013 02:38:45 PM Read time: 09/05/2013 02:38:45 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3231 Comment: Plate 19 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32236146 cps Min counts: 773873 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22843362.00 13080943.00 15078850.00 17360888.00 14758360.00 18606448.00 10955054.00 14742587.00 11821239.00 11991109.00 6014659.00 15588685.00 B 26318210.00 10379194.00 16310347.00 15310378.00 17580330.00 16283407.00 14984429.00 13538493.00 6314337.00 16397854.00 9246931.00 14068361.00 C 17958308.00 12514130.00 17040874.00 15852288.00 8174271.00 13672295.00 14939331.00 7842979.00 10636987.00 11216878.00 9465952.00 20659000.00 D 20140534.00 16878566.00 19201536.00 18855332.00 12947139.00 13297577.00 11508221.00 14287209.00 9509982.00 9144341.00 12111705.00 20545794.00 E 11702382.00 14368887.00 17532654.00 19150410.00 15979752.00 19608122.00 19465780.00 17600520.00 12462498.00 9421320.00 7900549.00 29516214.00 F 15748412.00 12394732.00 18390580.00 13848975.00 16301244.00 14870206.00 11576341.00 18245930.00 12285582.00 13224859.00 9257760.00 32236146.00 G 12649109.00 10270839.00 14365740.00 17586244.00 14794249.00 16232278.00 21327240.00 16224416.00 13822926.00 11724667.00 16175074.00 29958762.00 H 11816183.00 24649824.00 14531095.00 11252401.00 17570550.00 15090702.00 18292986.00 13155727.00 10373505.00 14491205.00 16124016.00 25260770.00