Load time: 02/22/2010 12:53:39 PM Read time: 02/22/2010 12:53:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #640 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 83732240 cps Min counts: 89 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34280404.00 36822272.00 18736058.00 25665850.00 9559761.00 32220206.00 83732240.00 76198720.00 898.00 359.00 718.00 628.00 B 15922991.00 16078625.00 16568400.00 25564796.00 14999606.00 28396048.00 62028088.00 53458680.00 718.00 898.00 359.00 449.00 C 15873237.00 17137958.00 17071832.00 8710747.00 10228446.00 33637792.00 35715284.00 32763242.00 718.00 628.00 718.00 269.00 D 14834560.00 13886559.00 11533682.00 13791108.00 15545176.00 14207971.00 20919196.00 19298442.00 269.00 898.00 628.00 359.00 E 14665550.00 15047478.00 718.00 11985499.00 16478581.00 898.00 11590048.00 11298168.00 628.00 179.00 718.00 898.00 F 15645297.00 14457202.00 718.00 30166904.00 11963071.00 359.00 6842946.00 6839713.00 898.00 628.00 987.00 449.00 G 15007358.00 17280256.00 718.00 28957004.00 12961561.00 808.00 3848691.00 3778184.00 898.00 449.00 538.00 269.00 H 14220137.00 15403031.00 179.00 20021982.00 16343038.00 129876.00 2263048.00 2214636.00 449.00 359.00 718.00 718.00 Load time: 02/22/2010 12:56:21 PM Read time: 02/22/2010 12:56:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #641 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 55914268 cps Min counts: 359 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34671416.00 35486228.00 31177418.00 30733092.00 35885996.00 32037266.00 54944988.00 52376196.00 47921528.00 48208440.00 42483848.00 42829768.00 B 23610072.00 36749184.00 30693630.00 35222988.00 31645532.00 33069558.00 31553276.00 53545276.00 48088460.00 46972408.00 41342204.00 46444640.00 C 30898102.00 37090608.00 30760898.00 30497224.00 26857288.00 37257116.00 44183776.00 48697436.00 39962196.00 48924684.00 36483368.00 47459172.00 D 37240184.00 39515108.00 38160856.00 35237864.00 36762152.00 31718380.00 50150704.00 52520204.00 50893180.00 54357632.00 40959296.00 43779180.00 E 42422360.00 40780728.00 37628048.00 31559392.00 35466352.00 718.00 52467400.00 48746340.00 48688928.00 47158340.00 51121600.00 1167.00 F 35343416.00 35945568.00 32750790.00 33954680.00 38016096.00 1437.00 51711624.00 47820116.00 48277900.00 47780244.00 45829952.00 1167.00 G 29265328.00 31510658.00 35353236.00 32073240.00 34342616.00 1077.00 48306048.00 44767284.00 49621128.00 47500532.00 41384292.00 987.00 H 22177012.00 46635856.00 41079580.00 40235220.00 39053392.00 1077.00 28085576.00 55914268.00 53796380.00 49916220.00 48742600.00 808.00 Load time: 02/22/2010 12:58:35 PM Read time: 02/22/2010 12:58:35 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #642 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 51982336 cps Min counts: 449 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39626276.00 39260112.00 40925764.00 39934344.00 41632176.00 43717892.00 26712938.00 24136210.00 20303232.00 18984740.00 19336734.00 21644988.00 B 23590026.00 44273312.00 39633320.00 42502108.00 37861124.00 51562392.00 19656360.00 28516978.00 24339760.00 21147754.00 16046245.00 22533644.00 C 32980856.00 44928232.00 34879264.00 43297280.00 36050708.00 51982336.00 23757890.00 25710532.00 22148930.00 21727014.00 15394632.00 22276702.00 D 42083552.00 45587912.00 46242176.00 47283992.00 45797660.00 45224936.00 31809816.00 31639192.00 28587112.00 30836750.00 27673382.00 21940110.00 E 40177040.00 48939260.00 42815808.00 46231952.00 47220868.00 7993.00 29192362.00 27907890.00 25411066.00 26611244.00 25823666.00 1077.00 F 41058080.00 44304132.00 45593220.00 42326412.00 45281764.00 1167.00 28020080.00 25631606.00 23201112.00 23786140.00 24700058.00 898.00 G 38399924.00 39779716.00 36972556.00 36453720.00 44075684.00 898.00 23869152.00 22876332.00 21454584.00 25060696.00 20220562.00 1257.00 H 16211891.00 44486440.00 41311100.00 14819686.00 41389136.00 1077.00 20697444.00 25112460.00 24711024.00 26574970.00 27385380.00 898.00 Load time: 02/22/2010 01:00:43 PM Read time: 02/22/2010 01:00:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #643 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 42870988 cps Min counts: 314 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26802364.00 31804204.00 23916432.00 25471766.00 16325060.00 29036470.00 35101904.00 36452820.00 33107324.00 25609770.00 30928290.00 37380752.00 B 14687565.00 31267698.00 25162322.00 24673502.00 25268214.00 26644550.00 21658238.00 38852420.00 33071908.00 27428686.00 20632080.00 29023984.00 C 24085348.00 32301598.00 25398364.00 31331714.00 22217632.00 29476878.00 32419982.00 35149520.00 29915516.00 31170522.00 23557896.00 32721222.00 D 34607716.00 31837976.00 31482936.00 24005542.00 28570106.00 27508032.00 38653296.00 38169880.00 36268288.00 40619396.00 35809356.00 30147476.00 E 29669744.00 34465708.00 28253578.00 26807834.00 29664290.00 808.00 38527800.00 36405204.00 35652296.00 29868128.00 31807724.00 1077.00 F 35041752.00 29311400.00 30658256.00 24302764.00 28261522.00 1077.00 38404252.00 37282112.00 34681872.00 31353664.00 35101768.00 628.00 G 26443474.00 25313346.00 23790276.00 22899456.00 25384468.00 1167.00 38824084.00 38115308.00 36740968.00 37489012.00 32224584.00 987.00 H 37621696.00 28337588.00 33950640.00 27025260.00 33766324.00 1167.00 25242378.00 42866488.00 38267356.00 42870988.00 39566272.00 898.00 Load time: 02/22/2010 01:02:46 PM Read time: 02/22/2010 01:02:46 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #644 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 55639188 cps Min counts: 404 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44408036.00 49429520.00 46818004.00 45831328.00 48734524.00 45329972.00 32263466.00 42830512.00 40845656.00 41541068.00 43186432.00 40589820.00 B 33525052.00 50711132.00 44906196.00 47735292.00 39018400.00 49341484.00 25779280.00 44386560.00 41883472.00 21375612.00 33636656.00 32362032.00 C 33767932.00 50820836.00 44915124.00 49610824.00 36788696.00 51304464.00 33963144.00 52292148.00 41396356.00 48793540.00 33035854.00 36432356.00 D 49543348.00 47082296.00 46836840.00 53720652.00 45227212.00 44871520.00 41621244.00 51150032.00 54663332.00 46117204.00 46675268.00 44445968.00 E 51167892.00 55639188.00 45016420.00 51113080.00 47842624.00 898.00 47903428.00 50407628.00 49792588.00 52281220.00 52136520.00 808.00 F 46920408.00 51648024.00 49333808.00 51389744.00 54372408.00 1616.00 44696472.00 48653900.00 50561496.00 45723040.00 50579012.00 808.00 G 45585820.00 50595148.00 49183368.00 50530732.00 48471080.00 1077.00 40461252.00 39449768.00 39190352.00 41470924.00 53675116.00 987.00 H 24520074.00 27538016.00 40257056.00 47783904.00 49008756.00 1077.00 41128308.00 42951776.00 39156272.00 46558428.00 38693340.00 987.00