Load time: 03/16/2010 02:56:32 PM Read time: 03/16/2010 02:56:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #670 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 199786672 cps Min counts: 134 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 86707456.00 85259032.00 90191112.00 1028735.00 1011304.00 199786672.00 89258848.00 628.00 449.00 808.00 359.00 628.00 B 51521900.00 53938940.00 54241584.00 575423.00 587823.00 48771576.00 51837860.00 269.00 269.00 359.00 539.00 269.00 C 30674434.00 32735620.00 31127004.00 342882.00 357707.00 46166364.00 30805658.00 718.00 539.00 898.00 449.00 539.00 D 18144840.00 21504230.00 20181748.00 214570.00 223466.00 48363772.00 18195110.00 539.00 718.00 359.00 449.00 449.00 E 11372798.00 12596286.00 11360248.00 158951.00 165870.00 70804.00 11418990.00 898.00 808.00 898.00 808.00 718.00 F 6911193.00 7038157.00 6795102.00 121662.00 142867.00 69456.00 6941833.00 898.00 988.00 359.00 539.00 359.00 G 4188623.00 4132195.00 4091851.00 107914.00 110969.00 77813.00 4017182.00 718.00 628.00 628.00 628.00 359.00 H 2663086.00 2746650.00 2736317.00 93537.00 96143.00 72961.00 2740091.00 718.00 718.00 808.00 269.00 808.00 Load time: 03/16/2010 03:00:10 PM Read time: 03/16/2010 03:00:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #671 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 41837396 cps Min counts: 58764 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 123548.00 121662.00 127502.00 21685138.00 22453696.00 16196230.00 23440434.00 28211892.00 34122740.00 31666038.00 31129006.00 30256282.00 B 121212.00 129659.00 122830.00 20598030.00 16109761.00 15995095.00 32768208.00 28904570.00 29602664.00 33920180.00 32061392.00 30638810.00 C 121662.00 127951.00 126693.00 18758176.00 19701810.00 21156916.00 23361884.00 32526224.00 34047732.00 37846664.00 37691432.00 36172460.00 D 125705.00 126514.00 122650.00 36410364.00 15897819.00 17562770.00 28689576.00 28185878.00 32744008.00 37284820.00 28235416.00 30859538.00 E 120943.00 124177.00 121302.00 21989574.00 17260356.00 117708.00 35429168.00 27817892.00 121841.00 38065912.00 30978138.00 119595.00 F 121212.00 123728.00 122290.00 17545230.00 18028574.00 117528.00 36518128.00 31917724.00 122111.00 33730204.00 34136224.00 124357.00 G 126334.00 126334.00 128041.00 22180876.00 20053300.00 208640.00 33114694.00 39071456.00 126693.00 41837396.00 33555892.00 131276.00 H 118606.00 122560.00 125346.00 24804220.00 16882206.00 127682.00 31499792.00 28551098.00 125975.00 32971880.00 28931224.00 121392.00 Load time: 03/16/2010 03:02:52 PM Read time: 03/16/2010 03:02:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #672 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 44423112 cps Min counts: 31673 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14781543.00 15965404.00 17966202.00 19511020.00 23032390.00 11881049.00 34944728.00 44423112.00 37082292.00 31316776.00 34783664.00 30843142.00 B 17351390.00 19455274.00 20067122.00 21027084.00 28418048.00 23958058.00 35622264.00 39343244.00 29420828.00 42661432.00 40906040.00 33191000.00 C 19271938.00 20010538.00 16533754.00 20388762.00 20497432.00 25390614.00 33271500.00 38955456.00 30937802.00 36817208.00 39030480.00 38086196.00 D 20961982.00 22408690.00 18917284.00 23479928.00 18867504.00 27616256.00 41880904.00 33348832.00 35817268.00 39852804.00 35971364.00 44145604.00 E 19816612.00 17194932.00 76735.00 25396712.00 20647058.00 73590.00 30815666.00 41546420.00 71793.00 35253512.00 40755156.00 68288.00 F 20022058.00 19344762.00 74488.00 25532322.00 21806568.00 71343.00 35630888.00 33224436.00 66941.00 36112408.00 34643820.00 68827.00 G 18258676.00 21471724.00 73500.00 19972434.00 21250306.00 75387.00 31866294.00 37322476.00 66132.00 27723472.00 34711520.00 63346.00 H 22983230.00 23460168.00 74938.00 26418226.00 22514410.00 70714.00 37793692.00 36055552.00 68378.00 29341576.00 31237888.00 64155.00 Load time: 03/16/2010 03:05:23 PM Read time: 03/16/2010 03:05:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #673 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 46680248 cps Min counts: 33740 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25329960.00 34784484.00 30060926.00 28274050.00 30286460.00 27957700.00 32297900.00 38977616.00 23002878.00 25754874.00 20102120.00 19715646.00 B 27243754.00 31489414.00 29710584.00 28325428.00 27678668.00 30163516.00 40854952.00 19095236.00 39933276.00 38178096.00 37947656.00 39633032.00 C 24593126.00 31830744.00 34343908.00 29760892.00 26281252.00 26852138.00 37674036.00 38485900.00 39777296.00 42090068.00 40772444.00 37142096.00 D 38810204.00 36205768.00 37723268.00 34300608.00 19910156.00 32910380.00 37798472.00 35375804.00 42485128.00 46680248.00 40998312.00 37040164.00 E 23447302.00 36165140.00 68917.00 34061568.00 31363052.00 76645.00 34104020.00 39177224.00 76824.00 43097652.00 36131092.00 78352.00 F 28840676.00 29650002.00 67480.00 31443732.00 28630318.00 77543.00 37668904.00 37760348.00 76016.00 45231560.00 35168620.00 78442.00 G 28093554.00 31601054.00 71883.00 27139212.00 28693106.00 74398.00 31979320.00 39361288.00 78352.00 44041436.00 37201716.00 76914.00 H 12286621.00 27386248.00 69816.00 34612312.00 24417516.00 78082.00 31323626.00 37139400.00 77723.00 46120316.00 33331496.00 76465.00 Load time: 03/16/2010 03:07:51 PM Read time: 03/16/2010 03:07:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #674 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 50895764 cps Min counts: 32077 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16744101.00 24826340.00 18115642.00 17784278.00 22842384.00 19929296.00 38016920.00 36247700.00 40913764.00 39467192.00 44682580.00 40320756.00 B 18981698.00 17957820.00 21606084.00 17345086.00 14300509.00 15315370.00 36837220.00 35848072.00 36377072.00 45385612.00 44725148.00 43004996.00 C 21270360.00 27149176.00 20965598.00 4758207.00 21265248.00 13263768.00 46501004.00 39645792.00 44674500.00 45032648.00 46991664.00 41388876.00 D 19350334.00 23809948.00 20764256.00 20235232.00 16894162.00 13107034.00 45775676.00 28739510.00 40447048.00 41360032.00 34035108.00 44924088.00 E 19111616.00 23888206.00 78622.00 19621708.00 16866432.00 74848.00 32663804.00 32876364.00 73140.00 46810092.00 47159960.00 84103.00 F 20536390.00 23757534.00 77094.00 14192499.00 16712540.00 77184.00 41720964.00 44386732.00 69367.00 48718380.00 45158852.00 68738.00 G 20329996.00 22110688.00 74398.00 17408942.00 22092810.00 73769.00 46254148.00 42640328.00 70714.00 41200804.00 50895764.00 66132.00 H 21429094.00 20481144.00 77004.00 21901024.00 19199112.00 78711.00 41465532.00 39167760.00 76285.00 43200352.00 29797444.00 64155.00 Load time: 03/16/2010 03:10:23 PM Read time: 03/16/2010 03:10:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #675 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 35882148 cps Min counts: 224 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20208688.00 28518284.00 24344864.00 30020348.00 29226260.00 28086332.00 150145.00 127772.00 132983.00 31235288.00 24552798.00 9358285.00 B 26113350.00 27927956.00 29549376.00 27639612.00 27519886.00 29504226.00 126514.00 131635.00 129299.00 12458837.00 12507499.00 12377527.00 C 25864034.00 26749540.00 30308780.00 29915346.00 24581888.00 24311350.00 130108.00 128131.00 129569.00 14355329.00 15889013.00 16588065.00 D 25664934.00 32701854.00 27293838.00 27758270.00 26809712.00 27535368.00 126154.00 129479.00 9128323.00 26318760.00 18981960.00 23914002.00 E 23650850.00 32223358.00 808.00 31728196.00 29193388.00 539.00 130198.00 125256.00 988.00 21735288.00 17063404.00 1078.00 F 25904592.00 32124694.00 1257.00 17885196.00 28060186.00 449.00 131186.00 129209.00 539.00 16019232.00 13124047.00 808.00 G 26693512.00 30547236.00 1437.00 28662360.00 28620902.00 898.00 131006.00 133253.00 539.00 35381076.00 14833582.00 898.00 H 21143408.00 16842734.00 449.00 17930674.00 14668530.00 718.00 123818.00 122919.00 628.00 35882148.00 27731748.00 628.00