Load time: 03/17/2010 12:25:06 PM Read time: 03/17/2010 12:25:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #677 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 92553120 cps Min counts: 63437 counts Well list: r0(d1:e12) = cps(f2(d1:e12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 92553120.00 60063212.00 36413176.00 21051444.00 11810643.00 6969327.00 3941852.00 2255632.00 1434563.00 838819.00 127484.00 126875.00 E 84258336.00 61632316.00 36557656.00 21076546.00 11743100.00 6971403.00 3994285.00 2248629.00 1451081.00 851569.00 131442.00 127998.00 F G H Load time: 03/17/2010 12:25:59 PM Read time: 03/17/2010 12:25:59 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #678 Comment: new comment Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 95613848 cps Min counts: 63951 counts Well list: r0(d1:e12) = cps(f2(d1:e12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 95613848.00 61098488.00 36766128.00 21358198.00 11964243.00 7073547.00 3991795.00 2281537.00 1456981.00 845574.00 131347.00 127902.00 E 86695832.00 62378548.00 37002968.00 21305830.00 11867102.00 7115915.00 4054512.00 2264103.00 1465868.00 850693.00 133554.00 132527.00 F G H Load time: 03/17/2010 12:28:05 PM Read time: 03/17/2010 12:28:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #679 Comment: plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 60830528 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44062948.00 39233844.00 21116344.00 22557534.00 40821920.00 33341278.00 50120392.00 38976064.00 33813208.00 27500984.00 35509620.00 17331496.00 B 30879760.00 49069412.00 39748620.00 32188070.00 27917022.00 29005180.00 33884196.00 28862982.00 33078110.00 30213548.00 51994256.00 20420480.00 C 29324130.00 11539304.00 36157620.00 30925458.00 41245284.00 42121660.00 37610548.00 38993224.00 26956688.00 34468100.00 33220774.00 19639560.00 D 27139710.00 25593468.00 45224876.00 39628180.00 41179488.00 60830528.00 31530926.00 39351692.00 29745786.00 38845732.00 44253320.00 8340188.00 E 39864692.00 27184576.00 25984218.00 45639816.00 35345060.00 30572132.00 23103576.00 50319052.00 46709432.00 37315800.00 18782254.00 27227684.00 F 29812112.00 28176774.00 20633346.00 41127124.00 42053028.00 47946604.00 36056928.00 40348588.00 25517892.00 36144372.00 20416360.00 22651270.00 G 34872892.00 53294296.00 40360292.00 41179488.00 32173056.00 24904942.00 39487252.00 45701088.00 37116084.00 33434374.00 20521334.00 24902872.00 H 12051178.00 30505012.00 27898238.00 56125516.00 34487620.00 53268800.00 40077864.00 44512600.00 43221740.00 45191312.00 19451026.00 19530612.00 Load time: 03/17/2010 12:30:26 PM Read time: 03/17/2010 12:30:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #680 Comment: plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 39819284 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20849468.00 20519280.00 33444560.00 21855850.00 31313816.00 19822910.00 14430716.00 19429692.00 14929981.00 27507678.00 13277570.00 24017032.00 B 33636072.00 24875298.00 23004844.00 17220688.00 22752230.00 28271922.00 16689412.00 19465940.00 8746426.00 17900656.00 16012768.00 16179418.00 C 26564868.00 31788748.00 23696072.00 28637750.00 25354460.00 34346448.00 24499234.00 29266220.00 22219186.00 16086362.00 16851376.00 29619416.00 D 24069164.00 21682764.00 35822952.00 23191288.00 29974788.00 25338636.00 17098336.00 25660450.00 12217113.00 21946246.00 31058910.00 36297536.00 E 29240090.00 16284744.00 27469776.00 20471694.00 39819284.00 18050928.00 24599358.00 34649820.00 24930670.00 23397548.00 24780876.00 29568856.00 F 35244700.00 28666820.00 31668378.00 20224126.00 33530880.00 10941379.00 15096622.00 28122084.00 29247000.00 23426802.00 27660836.00 23078676.00 G 25059714.00 28199100.00 21495964.00 22569942.00 31783304.00 17939920.00 31459916.00 35689932.00 13644011.00 23189078.00 19052798.00 26965736.00 H 21845130.00 33130550.00 34759524.00 29460898.00 14093916.00 20569640.00 19430564.00 13850817.00 30423690.00 34872576.00 31226382.00 21667212.00 Load time: 03/17/2010 12:33:03 PM Read time: 03/17/2010 12:33:03 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #681 Comment: plate 5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 61316216 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26578004.00 24294974.00 22608108.00 45679928.00 20814450.00 47168452.00 52779704.00 29066290.00 25099656.00 42045544.00 34106144.00 30086550.00 B 30926438.00 22864486.00 36159800.00 34446924.00 44432668.00 25102648.00 29510864.00 26046560.00 30071112.00 17762620.00 33626204.00 31476558.00 C 27934932.00 32757416.00 37974248.00 52768624.00 36183468.00 39959108.00 26806786.00 41278708.00 32975260.00 26132044.00 39854704.00 37567408.00 D 17960092.00 26802268.00 24410466.00 61316216.00 34354624.00 33952240.00 28955082.00 27749462.00 45688764.00 28109714.00 32702714.00 24999674.00 E 28672618.00 25419364.00 27183818.00 49310920.00 31742148.00 25541222.00 27593074.00 10314692.00 12048126.00 40033812.00 15669309.00 33874924.00 F 27069964.00 39401624.00 26327726.00 47920372.00 34459112.00 25488772.00 22608368.00 26375028.00 48955696.00 28875492.00 32184744.00 27103662.00 G 25104180.00 32156464.00 44355556.00 37290988.00 34628424.00 30902776.00 35461804.00 26346592.00 43049628.00 40046564.00 30672462.00 44070920.00 H 17789618.00 35235932.00 29722760.00 36000308.00 32958944.00 38894192.00 38065492.00 26040208.00 39389080.00 24991258.00 28457090.00 29326558.00 Load time: 03/17/2010 12:37:09 PM Read time: 03/17/2010 12:37:09 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #682 Comment: plate 6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59715348 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 32597138.00 25519732.00 27319964.00 17577762.00 28269360.00 29836416.00 6542316.00 29264130.00 38288372.00 17472990.00 32062158.00 24954774.00 B 33720908.00 23679370.00 20071542.00 11660013.00 20995988.00 20534364.00 36676860.00 35781676.00 15383143.00 17402384.00 25394772.00 28257574.00 C 24370932.00 21612306.00 18193374.00 28920916.00 37854204.00 20961378.00 30803978.00 40167288.00 41770440.00 20574906.00 28940564.00 21045364.00 D 59715348.00 38335828.00 30604418.00 33838160.00 29031648.00 30853234.00 45940000.00 38964568.00 38348272.00 26332972.00 30382542.00 23488076.00 E 20540854.00 21872286.00 19474012.00 29325786.00 39934540.00 44880116.00 33140408.00 18817756.00 32802768.00 40407784.00 46758188.00 19125724.00 F 25714118.00 12966498.00 19998006.00 20071076.00 27783482.00 24880536.00 32854340.00 29283702.00 31672368.00 32130750.00 46793836.00 17795912.00 G 22177840.00 18762994.00 17524450.00 33302388.00 21196272.00 27666824.00 28576644.00 24183644.00 13426225.00 28404204.00 35923328.00 32654492.00 H 19132156.00 20291990.00 18925858.00 27493124.00 30916502.00 12911942.00 33038428.00 12519352.00 18474648.00 24842948.00 32889828.00 15829720.00 Load time: 03/17/2010 12:39:25 PM Read time: 03/17/2010 12:39:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #683 Comment: plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53060664 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23940904.00 37946708.00 20775780.00 30040748.00 13282524.00 19062722.00 31249418.00 45979836.00 14775152.00 17382360.00 29204594.00 19317162.00 B 10589699.00 23602582.00 21422530.00 38339784.00 7880979.00 13108739.00 37976288.00 30553916.00 18432272.00 30045034.00 36892808.00 32784282.00 C 25602966.00 30237930.00 19659126.00 27942840.00 19172250.00 33652772.00 44145048.00 32217072.00 18781440.00 21927044.00 27726362.00 26802176.00 D 11351380.00 15035990.00 22002678.00 30864964.00 13007197.00 18417204.00 21561430.00 19362124.00 17460426.00 18900024.00 35252188.00 18618906.00 E 24368036.00 15381474.00 36844156.00 34819616.00 16605789.00 19042278.00 37454404.00 37490612.00 31279386.00 24289976.00 17884680.00 31956866.00 F 13476061.00 20453486.00 31704704.00 34926016.00 14842392.00 12334167.00 44867464.00 21038490.00 26588700.00 25795896.00 17339670.00 20036614.00 G 26815084.00 21058668.00 31655520.00 42265092.00 7582638.00 37297056.00 33727764.00 24478228.00 25112512.00 30793390.00 24817168.00 16773414.00 H 16214185.00 27272268.00 44568080.00 25108308.00 47681764.00 39745576.00 27603238.00 33495434.00 53060664.00 11493281.00 16712599.00 22494260.00 Load time: 03/17/2010 12:41:55 PM Read time: 03/17/2010 12:41:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #684 Comment: plate 8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40454764 cps Min counts: 465891 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15781071.00 20305100.00 24672002.00 19167676.00 23596294.00 24583222.00 23283488.00 4740384.00 3521910.00 4116948.00 4321601.00 11782614.00 B 24529686.00 25946678.00 24871566.00 16838164.00 24934418.00 21552308.00 7682565.00 7270124.00 3714603.00 4372594.00 8082839.00 21380986.00 C 21916506.00 26151246.00 21797786.00 21628494.00 23830820.00 28424090.00 11235197.00 4111629.00 2082978.00 3797633.00 3355752.00 19672324.00 D 21933276.00 33237916.00 13376849.00 31464614.00 34757044.00 22312236.00 3193517.00 3814770.00 2871474.00 4722858.00 5021347.00 14796382.00 E 31479356.00 27462226.00 30855070.00 13117276.00 32131808.00 25769290.00 5677166.00 2962916.00 2962397.00 5016229.00 5798909.00 16158122.00 F 40454764.00 11875744.00 16111665.00 30617042.00 29631126.00 27019856.00 931782.00 2623781.00 4482651.00 2796148.00 4307567.00 20019528.00 G 30739716.00 30595894.00 28112754.00 34160700.00 29490770.00 31203132.00 6621179.00 4094816.00 2417882.00 6853773.00 15380594.00 14190311.00 H 18848654.00 24392516.00 22664376.00 30337958.00 29093302.00 20153588.00 6307083.00 2615884.00 6668266.00 5508720.00 9144249.00 20422244.00 Load time: 03/17/2010 12:44:14 PM Read time: 03/17/2010 12:44:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #685 Comment: plate 9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 43722612 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17358398.00 30035888.00 22766318.00 10816049.00 21864190.00 20279744.00 16451682.00 7520099.00 21556960.00 18711872.00 16371276.00 32667082.00 B 14962767.00 26164852.00 16852906.00 9449875.00 36940192.00 15044668.00 18499200.00 17232642.00 21689944.00 26058660.00 27743634.00 30692390.00 C 26936196.00 14486823.00 34369920.00 13321251.00 22270096.00 10420664.00 22560472.00 11090199.00 12871623.00 33002622.00 16887262.00 20837094.00 D 25663574.00 27746496.00 23158902.00 16970540.00 23651136.00 25692420.00 17899302.00 14849234.00 12427218.00 11037168.00 23648482.00 43134672.00 E 24258000.00 24036656.00 11201036.00 17765656.00 8971220.00 30152188.00 17913618.00 15811931.00 23712654.00 18260886.00 16506727.00 24052972.00 F 20454452.00 22245500.00 13485760.00 26114098.00 29463572.00 8754304.00 19475278.00 26688018.00 19496954.00 20109964.00 8401640.00 24332970.00 G 20707782.00 18692442.00 29992316.00 26389316.00 17208102.00 21038036.00 12843882.00 27665350.00 23494236.00 16769589.00 25959122.00 20177274.00 H 19097516.00 7373439.00 14537697.00 43722612.00 36557656.00 11048291.00 11447252.00 25430396.00 16356676.00 19490226.00 20407114.00 27715420.00 Load time: 03/17/2010 12:46:32 PM Read time: 03/17/2010 12:46:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #686 Comment: plate 10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45173504 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10126093.00 14914867.00 20847682.00 23038162.00 24297550.00 41938544.00 45173504.00 16521913.00 15296024.00 29650852.00 7868305.00 29627634.00 B 20878618.00 21905664.00 18562328.00 20805894.00 11545868.00 30326630.00 42268752.00 31212504.00 29225072.00 25497944.00 10458540.00 27412198.00 C 26406926.00 29353172.00 15893596.00 37807020.00 23407036.00 18568520.00 22627140.00 23165026.00 14398249.00 19161834.00 38431084.00 21267930.00 D 30422740.00 21730880.00 38544724.00 24675284.00 4969372.00 27196240.00 19695436.00 23526784.00 30767016.00 32129688.00 33988808.00 29005610.00 E 22076398.00 40187996.00 21546886.00 26825328.00 15205080.00 8313840.00 23768546.00 13655866.00 31790434.00 34323008.00 17180606.00 34684820.00 F 19866660.00 31313942.00 15053269.00 29635634.00 31650892.00 39052812.00 16211757.00 20873532.00 11903764.00 18533420.00 9305175.00 22008768.00 G 13860050.00 6993795.00 29803786.00 26018128.00 8543564.00 10283490.00 19297940.00 17488592.00 15841399.00 35169324.00 7378114.00 27928824.00 H 25394274.00 23042110.00 25150838.00 24528840.00 22705638.00 34705512.00 7244677.00 18348490.00 9960391.00 40003404.00 22557076.00 29137892.00 Load time: 03/17/2010 12:48:44 PM Read time: 03/17/2010 12:48:44 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #687 Comment: plate 11 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53898652 cps Min counts: 779482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 31977976.00 21153362.00 33102128.00 31023062.00 22693276.00 34059948.00 15802666.00 15373223.00 17750686.00 22806420.00 19451028.00 17387672.00 B 22003800.00 35656300.00 46036048.00 27047892.00 27533426.00 22463210.00 27911524.00 9858380.00 26593236.00 24790408.00 17446434.00 7573892.00 C 27250436.00 35483272.00 53898652.00 18399032.00 21471922.00 20073300.00 14968083.00 18063934.00 22890000.00 22964314.00 33467966.00 12807458.00 D 34722208.00 33875216.00 30259760.00 17739980.00 22043248.00 20811892.00 13757431.00 20807558.00 18891184.00 24207150.00 20295370.00 11543046.00 E 20721104.00 19618402.00 38693588.00 20212852.00 24916964.00 19693944.00 18320414.00 16153301.00 13026631.00 11033934.00 16757510.00 8148178.00 F 22973182.00 45252968.00 48853820.00 19598328.00 17511262.00 18743188.00 15008835.00 17828394.00 25426664.00 20544050.00 18329288.00 24430742.00 G 11949771.00 26167048.00 46943196.00 23101316.00 23322362.00 17076086.00 17264586.00 28697636.00 12229995.00 37945972.00 19706638.00 12220963.00 H 33898348.00 33135480.00 34538356.00 29823286.00 29340248.00 23902876.00 20633508.00 16012570.00 14663583.00 16234682.00 5903769.00 13993594.00 Load time: 03/17/2010 12:51:13 PM Read time: 03/17/2010 12:51:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #688 Comment: standard 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 90695480 cps Min counts: 65806 counts Well list: r0(d1:e12) = cps(f2(d1:e12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 90695480.00 61888236.00 38200368.00 22007090.00 11830971.00 7261718.00 4092017.00 2331218.00 1496830.00 867783.00 133478.00 131613.00 E 86466952.00 63271724.00 37937108.00 21934202.00 12106433.00 7153623.00 4147104.00 2326210.00 1505489.00 877241.00 139206.00 136561.00 F G H