Load time: 03/18/2010 01:16:55 PM Read time: 03/18/2010 01:16:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #708 Comment: standard 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 87182480 cps Min counts: 70744 counts Well list: r0(d1:e12) = cps(f2(d1:e12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 81134640.00 59451340.00 35184168.00 20445508.00 11672887.00 7001111.00 3904895.00 711057.00 1426165.00 821205.00 145957.00 141488.00 E 87182480.00 62461352.00 36746528.00 21261680.00 12047277.00 7251098.00 4050706.00 2331311.00 1438945.00 852128.00 149552.00 144873.00 F G H Load time: 03/18/2010 01:18:24 PM Read time: 03/18/2010 01:18:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #709 Comment: plate 12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32669386 cps Min counts: 79549 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12289814.00 27778840.00 484009.00 21530902.00 10517175.00 22390936.00 10111523.00 16277485.00 12968978.00 18051498.00 17674110.00 19804216.00 B 14986826.00 10821325.00 159098.00 27287988.00 16468306.00 14056117.00 16812700.00 20244176.00 20008304.00 7840235.00 21993530.00 8877430.00 C 10036351.00 12647477.00 196125.00 24486636.00 14336816.00 20736702.00 27322350.00 9363372.00 12459042.00 18162196.00 24804932.00 9312450.00 D 14341223.00 18232166.00 608744.00 23439170.00 17486560.00 9483779.00 11402783.00 8790255.00 11003928.00 14081299.00 17178284.00 16186095.00 E 14627139.00 19011328.00 162522.00 16811214.00 15115045.00 14876034.00 12898050.00 9784627.00 18520570.00 14145868.00 20751176.00 13612592.00 F 18990238.00 14987548.00 168132.00 11504606.00 23780498.00 28665244.00 9543070.00 9724370.00 16604160.00 20432688.00 11334721.00 12143760.00 G 16289500.00 8459962.00 163853.00 18551048.00 18956178.00 17108558.00 16497779.00 15566222.00 11167220.00 15410671.00 32669386.00 19397546.00 H 24761014.00 20214858.00 162464.00 18626058.00 8586169.00 19388518.00 15560314.00 11870101.00 15638223.00 15126374.00 3938337.00 4578465.00 Load time: 03/18/2010 01:20:28 PM Read time: 03/18/2010 01:20:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #710 Comment: plate 13 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37511700 cps Min counts: 778949 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20834764.00 31464818.00 16561691.00 18059914.00 11960742.00 24042610.00 23752148.00 6612726.00 20641306.00 8940070.00 13090463.00 26531150.00 B 30402292.00 23779700.00 9221401.00 24439620.00 10287871.00 24100708.00 23310390.00 19132408.00 20194526.00 13737768.00 20958540.00 19895060.00 C 28552186.00 28465270.00 18166132.00 26647416.00 13307616.00 15629218.00 13069006.00 12943679.00 25235338.00 20372036.00 34486468.00 16255643.00 D 22426326.00 14442826.00 19319322.00 27525000.00 15825355.00 26764982.00 22570652.00 17646122.00 12561794.00 21905148.00 18195966.00 7292700.00 E 10780948.00 24594566.00 11224102.00 37511700.00 10501706.00 21916860.00 12549711.00 19900296.00 13361052.00 18842328.00 15563236.00 19704174.00 F 10974069.00 15142341.00 19178054.00 11586817.00 21222346.00 29522318.00 19468186.00 32164992.00 15706078.00 18512912.00 17927940.00 23985382.00 G 23901114.00 26055824.00 21748642.00 29959126.00 25178160.00 24510214.00 29019686.00 34002480.00 10892583.00 14438650.00 17209252.00 15312548.00 H 13825546.00 20518968.00 28364724.00 15327311.00 7285810.00 27094546.00 14765329.00 22467530.00 15350872.00 13162174.00 25795840.00 28411802.00 Load time: 03/18/2010 01:22:34 PM Read time: 03/18/2010 01:22:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #711 Comment: plate 14 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 44741228 cps Min counts: 778949 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14667821.00 23695716.00 11581064.00 20722080.00 16544840.00 24374156.00 27016456.00 29733158.00 22346420.00 28284802.00 19642162.00 18061754.00 B 26261112.00 12847101.00 14615201.00 15195837.00 20540068.00 13478987.00 21312582.00 34712688.00 20169582.00 20522158.00 25920222.00 13482019.00 C 19682366.00 31449570.00 13449221.00 22607596.00 16901970.00 15322999.00 44741228.00 26501184.00 28109750.00 25110712.00 16308839.00 21329506.00 D 26735218.00 18241816.00 14058731.00 17716318.00 12355468.00 18290476.00 27762604.00 37138452.00 36764568.00 18134204.00 26505062.00 27267930.00 E 30980144.00 30154782.00 17886074.00 23365348.00 11292049.00 29596800.00 28147334.00 21887240.00 16953842.00 34900412.00 19774200.00 9996526.00 F 12139349.00 11317167.00 34805908.00 29185936.00 13820248.00 22591728.00 24027556.00 24343838.00 20596716.00 21600820.00 34258352.00 21911864.00 G 29612326.00 19766424.00 16392684.00 22675712.00 14946595.00 16086848.00 34913084.00 22762384.00 23925784.00 31618846.00 27622902.00 24405314.00 H 19022796.00 13436391.00 17807038.00 21328572.00 16269359.00 13366554.00 32898152.00 21719350.00 17159438.00 25835968.00 10938410.00 23088532.00 Load time: 03/18/2010 01:24:43 PM Read time: 03/18/2010 01:24:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #712 Comment: plate 15 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 43278328 cps Min counts: 77153 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34373240.00 24726508.00 27665972.00 14756376.00 18129730.00 27969150.00 25951826.00 19462978.00 31072706.00 31108944.00 17390248.00 23061528.00 B 17580846.00 28720944.00 16147874.00 25739328.00 21965868.00 35353272.00 20214388.00 17657082.00 21415650.00 23520988.00 15020725.00 24774404.00 C 19483864.00 35655400.00 16588602.00 26911824.00 21006186.00 43278328.00 16237345.00 34380220.00 24889260.00 36270348.00 21814908.00 14507005.00 D 27311240.00 154858.00 32512302.00 25783974.00 20389314.00 21540490.00 22950978.00 32299704.00 22850056.00 29577246.00 32125860.00 28676534.00 E 19805070.00 18904150.00 12556610.00 12767797.00 18706576.00 29192934.00 22192984.00 31028890.00 36622656.00 37870616.00 27538722.00 33028576.00 F 18318606.00 11110450.00 28423826.00 13058094.00 23493536.00 18341424.00 29395310.00 22481276.00 40300140.00 30455306.00 28317912.00 26070040.00 G 16408225.00 27472772.00 23886676.00 7856509.00 7356045.00 18154618.00 18351320.00 20577672.00 16272215.00 32999750.00 12653887.00 29176972.00 H 154306.00 15391822.00 23744474.00 15710323.00 20339748.00 38246972.00 17279572.00 34007536.00 33338292.00 32849038.00 39391204.00 27398080.00 Load time: 03/18/2010 01:26:53 PM Read time: 03/18/2010 01:26:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #713 Comment: plate 16 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42151164 cps Min counts: 694721 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28951196.00 20691586.00 20263660.00 24869794.00 8563694.00 6165695.00 1624916.00 6671440.00 10603013.00 9735539.00 16975194.00 8476154.00 B 4582378.00 16560917.00 29001638.00 5279430.00 5430292.00 1742998.00 8220190.00 11024391.00 7293225.00 4167757.00 8194471.00 13735055.00 C 32854028.00 18172702.00 42151164.00 32478820.00 5136074.00 7932831.00 5267595.00 4695755.00 9285199.00 7658133.00 5003046.00 5454196.00 D 24008744.00 27472964.00 37638768.00 19001312.00 1548161.00 7112101.00 9520000.00 12303558.00 3001718.00 3804494.00 12324269.00 7076718.00 E 30071662.00 24794984.00 39437872.00 23448764.00 9156267.00 3427626.00 1389443.00 6326769.00 5438963.00 2593995.00 13335067.00 9475749.00 F 23806374.00 19383548.00 22938812.00 17644444.00 8607307.00 10706853.00 10002431.00 3879275.00 5305337.00 6353350.00 4182889.00 11327501.00 G 27862706.00 36498592.00 32634210.00 6186782.00 4436703.00 9032458.00 3084873.00 8913412.00 7082741.00 4959397.00 5263601.00 3497538.00 H 36559232.00 17361140.00 23389412.00 2319639.00 4684628.00 8668901.00 7938797.00 1767051.00 4504365.00 6282371.00 3844595.00 7284638.00 Load time: 03/18/2010 01:29:20 PM Read time: 03/18/2010 01:29:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #714 Comment: plate 17 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42983388 cps Min counts: 646474 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2665588.00 1524713.00 6156831.00 4616887.00 22380578.00 8473499.00 15022666.00 6841356.00 18891682.00 22031350.00 12864032.00 14203102.00 B 5669204.00 2937394.00 6806819.00 15882792.00 18648980.00 23908010.00 16052502.00 20019976.00 16981042.00 28836836.00 10691570.00 13886301.00 C 5732749.00 1708894.00 4033083.00 21852240.00 21510450.00 9937036.00 13013330.00 9950363.00 9999272.00 9142684.00 27373430.00 17214958.00 D 7474753.00 2490626.00 2248054.00 20980896.00 22812848.00 19089836.00 14044131.00 9556252.00 13260155.00 18718938.00 42983388.00 29091976.00 E 2383071.00 2792367.00 3580442.00 26265808.00 20780680.00 15279297.00 11859764.00 7493679.00 20166660.00 16064221.00 28300422.00 21393006.00 F 1292949.00 2322400.00 4382351.00 13553285.00 19188824.00 19559450.00 9619281.00 19243340.00 14833767.00 24151670.00 11291051.00 23575234.00 G 5606549.00 2869032.00 5553554.00 22445518.00 23870646.00 24931402.00 15257480.00 22214754.00 19345616.00 30414518.00 19478894.00 24121230.00 H 3013611.00 4586687.00 20480448.00 26993614.00 23437762.00 13545859.00 23372708.00 22631310.00 22804568.00 36573824.00 16577302.00 21356464.00 Load time: 03/18/2010 01:31:39 PM Read time: 03/18/2010 01:31:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #715 Comment: plate 18 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41363728 cps Min counts: 778949 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16873668.00 8421164.00 8089609.00 13529931.00 28273406.00 19437724.00 11326547.00 4251110.00 26690878.00 29584212.00 15000708.00 20894282.00 B 24921754.00 20251912.00 9088153.00 34504492.00 22071304.00 29192608.00 18141468.00 4885937.00 23418312.00 16544279.00 29969962.00 22252198.00 C 27697450.00 16184815.00 16674715.00 27924934.00 16178024.00 16524624.00 10407249.00 23905222.00 30923078.00 19699088.00 14750872.00 16631279.00 D 24992678.00 30396718.00 22813116.00 18480278.00 14335100.00 17509948.00 17050886.00 1814351.00 31497640.00 19828868.00 41363728.00 21349792.00 E 19400012.00 21091960.00 19268140.00 13208060.00 22537412.00 29154256.00 11359864.00 14773757.00 16661412.00 22390680.00 25373436.00 27818722.00 F 24491100.00 19488446.00 18593426.00 24018590.00 22005832.00 23008396.00 14425201.00 33688228.00 27079384.00 25924274.00 18815882.00 14607664.00 G 22611270.00 26722468.00 21148652.00 19822358.00 30322054.00 23222480.00 19529782.00 22129368.00 14389970.00 19463076.00 3702469.00 15689185.00 H 22230796.00 26805508.00 14022717.00 17557030.00 11601900.00 19365816.00 5855449.00 21891666.00 15354260.00 28128328.00 27280534.00 16018039.00 Load time: 03/18/2010 01:33:58 PM Read time: 03/18/2010 01:33:58 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #716 Comment: plate 19 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35355036 cps Min counts: 778949 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22416644.00 23079090.00 18125426.00 13715490.00 24585950.00 27043094.00 25370462.00 10331413.00 14969660.00 25988020.00 24506898.00 21974794.00 B 20167704.00 18161432.00 20081344.00 21589264.00 17253430.00 22722614.00 27618790.00 7684514.00 16047442.00 21995704.00 18970030.00 18109624.00 C 13785957.00 23898986.00 23272224.00 18586728.00 27651830.00 33354572.00 8134169.00 14331962.00 30850698.00 21763832.00 28907036.00 14249011.00 D 10452497.00 20510054.00 18594270.00 14931838.00 20425296.00 15940647.00 22812648.00 15456294.00 15064502.00 27882452.00 16469771.00 19651184.00 E 9354364.00 15918495.00 17165488.00 16847284.00 11991769.00 17270762.00 10642344.00 21545792.00 16955206.00 22578304.00 16193394.00 25728362.00 F 16164729.00 27944268.00 17271754.00 15947206.00 24289790.00 16980784.00 5960049.00 35355036.00 10168759.00 30878092.00 27069408.00 8342322.00 G 18402694.00 17714102.00 17714586.00 12283049.00 15338206.00 20672916.00 16305151.00 29146294.00 18041380.00 16568172.00 29541572.00 27392200.00 H 16746665.00 16409330.00 19066330.00 27947578.00 34291684.00 23513178.00 21276374.00 10233296.00 29322166.00 22510576.00 28007366.00 7211712.00 Load time: 03/18/2010 01:36:24 PM Read time: 03/18/2010 01:36:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #717 Comment: plate 20 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35268276 cps Min counts: 778949 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22336934.00 15070623.00 5050438.00 19289900.00 11437224.00 9841369.00 15163563.00 3097439.00 32089860.00 16534095.00 23160198.00 20318206.00 B 19902938.00 24956164.00 26412760.00 14566307.00 17391880.00 19620642.00 23264926.00 4360988.00 15680753.00 22968910.00 13592639.00 13637879.00 C 9130916.00 30882132.00 20236128.00 22762054.00 30246934.00 10225142.00 15012996.00 7739195.00 32138452.00 16602390.00 19734022.00 26447286.00 D 7229449.00 28692272.00 2794825.00 19654058.00 19329866.00 9635703.00 17735358.00 8246479.00 11984333.00 32719062.00 23963322.00 12639330.00 E 13847273.00 23229614.00 26900206.00 17947642.00 12510368.00 23783696.00 13658085.00 12687897.00 14414415.00 34471204.00 20724618.00 15463076.00 F 21143026.00 16488560.00 19367548.00 12024571.00 16419084.00 20554054.00 11207646.00 24776294.00 31140658.00 22286514.00 12019691.00 13281792.00 G 12463468.00 23473640.00 17485304.00 16415554.00 16603417.00 22338410.00 6577874.00 11983044.00 35268276.00 20405604.00 30327246.00 9928625.00 H 4413285.00 15624797.00 23341542.00 11959879.00 17159440.00 32059880.00 20340968.00 19900802.00 10061929.00 27772602.00 14225124.00 34348992.00 Load time: 03/18/2010 01:38:49 PM Read time: 03/18/2010 01:38:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #718 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 84953736 cps Min counts: 74205 counts Well list: r0(d1:e12) = cps(f2(d1:e12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 77801568.00 59223524.00 35481296.00 20676756.00 11799025.00 7179395.00 3965147.00 723875.00 1433468.00 836647.00 148639.00 148411.00 E 84953736.00 62267628.00 37580108.00 21878508.00 12333539.00 7292542.00 4101047.00 2349234.00 1450108.00 862340.00 153450.00 151358.00 F G H Load time: 03/18/2010 01:45:31 PM Read time: 03/18/2010 01:45:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #719 Comment: plate 12 re-do Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 63675020 cps Min counts: 778949 counts Well list: r0(a1:h3,a5:h6) = cps(f2(a1:h3,a5:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16261004.00 28952916.00 24402944.00 59949608.00 34922632.00 B 12884438.00 7288735.00 31030990.00 57821588.00 35201176.00 C 15227326.00 26084006.00 28127514.00 59812888.00 33717536.00 D 20375446.00 29901510.00 28646162.00 63675020.00 34396008.00 E 21443890.00 25733714.00 20631630.00 6474916.00 4440158.00 F 16245100.00 29527688.00 14190346.00 6660237.00 8809232.00 G 11002311.00 20265086.00 22327908.00 6762798.00 4314927.00 H 22183188.00 18368976.00 22515394.00 6836690.00 4369376.00