Load time: 03/30/2010 02:39:19 PM Read time: 03/30/2010 02:39:19 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #747 Comment: Plate 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 85536024 cps Min counts: 2482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43425872.00 43474088.00 44355852.00 715424.00 706655.00 717878.00 85536024.00 74040080.00 77005752.00 6239.00 205983.00 6753.00 B 27498152.00 31848276.00 32117126.00 480297.00 482942.00 496791.00 55877976.00 53810376.00 54363384.00 8655.00 9321.00 9264.00 C 19868234.00 19050688.00 18674336.00 347002.00 353260.00 353127.00 35596536.00 32161934.00 32946320.00 8731.00 10672.00 7114.00 D 11840540.00 11848894.00 11680771.00 263604.00 261245.00 265868.00 19401378.00 20375092.00 19388776.00 4965.00 10557.00 8636.00 E 7052741.00 6918081.00 7103807.00 215209.00 217206.00 215494.00 11598460.00 11600481.00 10981442.00 5802.00 10995.00 8275.00 F 4256049.00 4271980.00 4088090.00 188862.00 193694.00 192020.00 6753616.00 6898182.00 6552246.00 5973.00 9739.00 7304.00 G 2586617.00 2533995.00 2506157.00 179826.00 184506.00 181899.00 3733727.00 3728057.00 3785883.00 6125.00 9834.00 6620.00 H 1591242.00 1566651.00 1598738.00 158596.00 161430.00 160993.00 2194432.00 2126148.00 2223175.00 6905.00 6981.00 6448.00 Load time: 03/30/2010 02:42:07 PM Read time: 03/30/2010 02:42:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #748 Comment: Plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55552096 cps Min counts: 779190 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22576458.00 23377488.00 28904724.00 31357392.00 28544284.00 27940792.00 33540686.00 25779068.00 26956102.00 41553056.00 46583072.00 46141436.00 B 20565788.00 33447388.00 27740846.00 29429068.00 30885690.00 29221466.00 26468954.00 31043320.00 28687522.00 37357980.00 36030428.00 50278128.00 C 22338020.00 31353354.00 29735892.00 32147738.00 32516804.00 30755252.00 34417904.00 37343304.00 34509976.00 41824496.00 48910936.00 40927092.00 D 18702036.00 30841680.00 37950232.00 34082484.00 31766606.00 37499656.00 35194420.00 38694460.00 33030682.00 46931264.00 42333044.00 52201816.00 E 23769936.00 22168776.00 33616220.00 30965596.00 40366704.00 31736460.00 31400988.00 37989456.00 26064760.00 43987756.00 55552096.00 48365076.00 F 26682042.00 28278772.00 33194752.00 34221844.00 33774320.00 26282266.00 27113404.00 34219140.00 34599008.00 35223376.00 36143904.00 52921544.00 G 29947666.00 27565372.00 39184044.00 33807728.00 26944824.00 30195210.00 47713808.00 39893624.00 37737028.00 35991320.00 49084404.00 13696510.00 H 29680050.00 37100064.00 34621760.00 35574432.00 39066556.00 41313784.00 26760118.00 40310108.00 31683036.00 41557928.00 48325184.00 41216752.00 Load time: 03/30/2010 02:44:11 PM Read time: 03/30/2010 02:44:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #749 Comment: Plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 135894256 cps Min counts: 188519 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39697900.00 61623356.00 70941256.00 68743192.00 67043856.00 69197400.00 67191280.00 73533496.00 59404904.00 97362296.00 68511696.00 113379672.00 B 71242872.00 67888512.00 81162280.00 70670384.00 80528984.00 94222328.00 50851364.00 90242560.00 79044632.00 106245024.00 101331736.00 120724224.00 C 66953408.00 79089560.00 63722340.00 92895688.00 100617304.00 26398290.00 38000576.00 93405704.00 87769400.00 88430816.00 116736144.00 95327808.00 D 79500304.00 77844312.00 87655856.00 85274848.00 89242072.00 5228543.00 27518352.00 100549776.00 87334576.00 126551528.00 130469560.00 95541704.00 E 71283936.00 80685752.00 85141608.00 73356960.00 82379912.00 94368400.00 77416608.00 107758432.00 87033152.00 103686064.00 110991040.00 114801232.00 F 62536348.00 89261496.00 69399552.00 74942320.00 100943160.00 108629760.00 95249728.00 98357784.00 135894256.00 117263184.00 106672656.00 101938904.00 G 77200304.00 82463600.00 104397336.00 99827104.00 73640528.00 83254840.00 102670944.00 91224072.00 106678496.00 101767144.00 103918368.00 99909016.00 H 61791472.00 68738344.00 63209032.00 80833072.00 89076800.00 80412624.00 72530728.00 75773880.00 90426880.00 89088008.00 377039.00 103474016.00 Load time: 03/30/2010 02:46:22 PM Read time: 03/30/2010 02:46:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #750 Comment: Plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41067732 cps Min counts: 237447 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24171286.00 8854226.00 4973170.00 12058392.00 41067732.00 34737828.00 31124416.00 8600649.00 5355974.00 10665645.00 32310638.00 29094894.00 B 10733863.00 6122090.00 15339359.00 16283442.00 14042199.00 27836280.00 10876669.00 6239583.00 15602969.00 15219929.00 13593692.00 25635224.00 C 21312938.00 8613550.00 7887245.00 15810851.00 14733590.00 13051000.00 20578552.00 9293447.00 8770834.00 15300803.00 14401369.00 11845148.00 D 10323466.00 1854710.00 4244885.00 3922665.00 7211053.00 23682448.00 10533400.00 1625679.00 4696861.00 4409535.00 6344578.00 21588766.00 E 20539280.00 9107618.00 2168621.00 9826513.00 6759123.00 474895.00 21143662.00 8356771.00 2052636.00 9597418.00 5776051.00 478034.00 F 19125508.00 20868116.00 19315962.00 5908727.00 10206830.00 675076.00 17070812.00 17304268.00 19777510.00 6022323.00 10577069.00 667010.00 G 12235339.00 1946003.00 8706991.00 7460458.00 21301576.00 1690707.00 12302605.00 1835401.00 9485549.00 7193883.00 19628248.00 1657032.00 H 16571360.00 26673366.00 5399342.00 12088981.00 21945006.00 638209.00 16392689.00 26467608.00 6012852.00 12070309.00 21757376.00 675304.00 Load time: 03/30/2010 02:48:38 PM Read time: 03/30/2010 02:48:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #751 Comment: Plate 5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 74764000 cps Min counts: 75569 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21828324.00 8312640.00 4765221.00 10844908.00 33813448.00 24483520.00 24389334.00 23756238.00 18547074.00 21105974.00 9102553.00 25670952.00 B 9105617.00 6006556.00 15899249.00 17140848.00 14361262.00 25459994.00 22108268.00 21178488.00 16432411.00 5738624.00 20933318.00 18036438.00 C 20782680.00 9485018.00 8401183.00 14815702.00 14696437.00 10869462.00 21147274.00 22598264.00 15721244.00 15069691.00 10017850.00 22215296.00 D 10011750.00 1823001.00 4397388.00 4594529.00 6748141.00 19566440.00 19490088.00 14550653.00 17171068.00 36372352.00 14490904.00 20813376.00 E 19384918.00 9159887.00 2053751.00 9643815.00 6159467.00 495250.00 27787242.00 15859283.00 161031.00 7773419.00 7240658.00 52300628.00 F 18490868.00 20103262.00 20222482.00 5911058.00 10267393.00 666344.00 34731328.00 18799816.00 151139.00 12248302.00 24542588.00 55740868.00 G 12032305.00 1977657.00 9828570.00 7580639.00 19105856.00 1564983.00 15261665.00 20386236.00 158596.00 10555832.00 16851950.00 54749952.00 H 16289775.00 28301674.00 5896448.00 13138653.00 22075072.00 614696.00 14829040.00 18389752.00 152014.00 15190465.00 17640356.00 74764000.00 Load time: 03/30/2010 02:50:55 PM Read time: 03/30/2010 02:50:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #752 Comment: Plate 6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28716808 cps Min counts: 68730 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21966104.00 23182254.00 18478064.00 20584368.00 8999295.00 25845080.00 24981738.00 26133034.00 18424366.00 20135420.00 9635288.00 25359896.00 B 21052884.00 19686518.00 15660357.00 5331374.00 21792732.00 17828158.00 21680674.00 22195740.00 16545955.00 5367741.00 19362280.00 17954894.00 C 20980382.00 21681278.00 15139994.00 13575077.00 9886622.00 22279004.00 20964408.00 23834210.00 14679271.00 14024684.00 9478268.00 22180072.00 D 20236398.00 13427488.00 15171300.00 8087510.00 10282419.00 21230420.00 19212088.00 15536390.00 14752086.00 9657466.00 13451223.00 20602002.00 E 28716808.00 14430466.00 143320.00 7341039.00 6423923.00 140809.00 27399910.00 16093655.00 140961.00 7684270.00 6836065.00 139554.00 F 25779238.00 16967472.00 143492.00 10902327.00 23024838.00 137937.00 24857412.00 19529758.00 137461.00 10896915.00 24699508.00 140201.00 G 14740001.00 17744484.00 146916.00 10396288.00 16321679.00 147068.00 14309017.00 19781226.00 144252.00 10078036.00 15954364.00 158349.00 H 13068709.00 16521993.00 175184.00 15259124.00 16779298.00 146783.00 13361910.00 16468703.00 148476.00 14754291.00 16658125.00 140220.00 Load time: 03/30/2010 02:53:24 PM Read time: 03/30/2010 02:53:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #753 Comment: Plate 1 sensitivity 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 83682336 cps Min counts: 1572 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45512016.00 45687340.00 46232020.00 755519.00 741683.00 749679.00 83682336.00 74328040.00 77485560.00 4851.00 224706.00 4941.00 B 28445290.00 33721560.00 33763976.00 507992.00 511496.00 517067.00 57940332.00 55566852.00 56426180.00 7547.00 7816.00 8445.00 C 21483828.00 20352512.00 19854436.00 364327.00 369359.00 370886.00 37029996.00 33357312.00 34206900.00 8894.00 8715.00 5840.00 D 12730712.00 12633727.00 12472315.00 278254.00 271246.00 278524.00 20311166.00 20984968.00 20042148.00 3144.00 9344.00 6648.00 E 7751337.00 7357561.00 7551930.00 224796.00 227940.00 224975.00 11888685.00 11906432.00 11399216.00 4222.00 8804.00 6379.00 F 4544529.00 4557108.00 4357379.00 199639.00 201166.00 200447.00 7062210.00 7104258.00 6958976.00 4043.00 8265.00 6289.00 G 2760446.00 2708964.00 2666287.00 191103.00 191463.00 187240.00 3898802.00 3899341.00 3957921.00 4582.00 8894.00 4941.00 H 1712116.00 1683006.00 1708432.00 169181.00 169001.00 170438.00 2273387.00 2195670.00 2300431.00 5480.00 5390.00 4851.00 Load time: 03/30/2010 02:56:05 PM Read time: 03/30/2010 02:56:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #754 Comment: Plate 2 sensitivity 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 56387784 cps Min counts: 3680115 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23285632.00 23897628.00 29649390.00 31815090.00 29249652.00 28582130.00 33946992.00 26320358.00 27099004.00 42141584.00 47442692.00 46950360.00 B 20964658.00 34284624.00 28215640.00 30378196.00 31476034.00 30048918.00 26899598.00 31441668.00 29810356.00 37745120.00 36719672.00 51342612.00 C 22841430.00 31726226.00 30109668.00 32699316.00 33197138.00 30863506.00 35551308.00 37958424.00 35042304.00 42921096.00 49669804.00 41611292.00 D 19097916.00 31353512.00 38716660.00 34781856.00 32089874.00 38319368.00 35629856.00 39550376.00 33409970.00 47468204.00 43172200.00 53444136.00 E 23978644.00 22318444.00 34418980.00 31258038.00 41380060.00 32852156.00 32125396.00 38756208.00 26526084.00 44741404.00 56387784.00 49184864.00 F 28006068.00 28801930.00 33629852.00 34196824.00 34155784.00 26737680.00 26915594.00 34708864.00 35201592.00 35624784.00 36869580.00 54153588.00 G 30599848.00 27877210.00 39905692.00 34306488.00 27620612.00 30757978.00 48494224.00 40650508.00 38251972.00 36413492.00 50222656.00 14112310.00 H 30241696.00 37760916.00 35086108.00 35976952.00 39696664.00 42023060.00 28584972.00 41626868.00 32473416.00 41953692.00 48679284.00 41454592.00