Load time: 04/09/2010 11:05:47 AM Read time: 04/09/2010 11:05:47 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #758 Comment: Plate 2 sensitivity 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 106040792 cps Min counts: 67086 counts Well list: r0(d1:f12) = cps(f2(d1:f12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A B C D 80792064.00 49719840.00 29963844.00 16895786.00 10615954.00 5910513.00 3377410.00 1948243.00 134172.00 136509.00 1428268.00 828041.00 E 79588152.00 51536556.00 29108904.00 16363294.00 11389365.00 5638844.00 3147079.00 1764374.00 140373.00 140553.00 1341815.00 771874.00 F 97736560.00 106040792.00 97718136.00 137947.00 141901.00 140822.00 139025.00 140013.00 143518.00 152954.00 142620.00 143339.00 G H Load time: 04/09/2010 11:07:35 AM Read time: 04/09/2010 11:07:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #759 Comment: Plate 23 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 99412680 cps Min counts: 91350 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 63517144.00 56614140.00 51828924.00 48297424.00 72574880.00 55525472.00 60622656.00 52837668.00 46909136.00 85405248.00 62929892.00 85191192.00 B 43430616.00 61543276.00 51350140.00 58600756.00 47579440.00 73506032.00 76020392.00 66004824.00 56314804.00 48524276.00 99412680.00 88787616.00 C 47043180.00 56050524.00 75045992.00 45177356.00 47626104.00 61952204.00 42572488.00 55816788.00 55223916.00 78435784.00 99210400.00 77571792.00 D 72942040.00 61586316.00 70067264.00 57677224.00 48322216.00 55655772.00 53386816.00 67712552.00 63153056.00 75663184.00 71719392.00 409797.00 E 61044300.00 63625168.00 65945336.00 52397144.00 77753672.00 84561216.00 73028296.00 55571912.00 54813168.00 81767280.00 96529120.00 189621.00 F 78447144.00 73953936.00 44527356.00 57072252.00 58663140.00 53745536.00 47399192.00 75933048.00 63719448.00 65576340.00 97971712.00 182701.00 G 70857088.00 58212740.00 45967472.00 71325416.00 80784264.00 81263656.00 52457036.00 60427904.00 58277160.00 66765276.00 75386272.00 281555.00 H 64535520.00 70383032.00 63658836.00 58593484.00 74037680.00 77891384.00 52065564.00 52317464.00 64851484.00 77009800.00 76890440.00 227275.00 Load time: 04/09/2010 11:10:25 AM Read time: 04/09/2010 11:10:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #760 Comment: Plate 24 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 108375152 cps Min counts: 3680983 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44456372.00 108375152.00 39226044.00 64674188.00 64826592.00 42848268.00 36695860.00 41839532.00 60087588.00 53572048.00 50641144.00 66539228.00 B 46346684.00 52376420.00 39838260.00 86909936.00 90829280.00 46795120.00 70879192.00 42244168.00 67343400.00 53605432.00 46956768.00 99600712.00 C 42868276.00 64266120.00 34905264.00 64955164.00 36413224.00 45965520.00 71002376.00 45564000.00 51896880.00 63475960.00 59551660.00 58732416.00 D 69361560.00 38802092.00 40385704.00 54165484.00 77744152.00 47129556.00 44707288.00 39860000.00 33266336.00 38820052.00 71562112.00 104046952.00 E 42542868.00 43419856.00 40525004.00 69940784.00 69707280.00 41191636.00 47537840.00 46263736.00 50646376.00 47373572.00 87863368.00 103163112.00 F 43828688.00 40008948.00 51114972.00 47431868.00 41182696.00 34243936.00 58060496.00 43763348.00 76690520.00 47026476.00 83065544.00 85007632.00 G 40336716.00 38812072.00 58402256.00 73626440.00 37233216.00 66128380.00 67420856.00 48996360.00 65794112.00 84526848.00 92854704.00 75904704.00 H 39708944.00 38449996.00 64551820.00 37775332.00 51954380.00 37725160.00 36215180.00 62919676.00 51497416.00 37315384.00 60542992.00 82412232.00 Load time: 04/09/2010 11:12:37 AM Read time: 04/09/2010 11:12:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #761 Comment: Plate 25 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 125268712 cps Min counts: 3680983 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 54638804.00 64353640.00 67177728.00 35352464.00 94516512.00 82755360.00 93595352.00 71816080.00 66534780.00 52004664.00 68182392.00 64911640.00 B 57603572.00 125268712.00 82272784.00 92089512.00 66680376.00 68900528.00 79662368.00 85393368.00 100839464.00 92750360.00 62227336.00 69973760.00 C 75963128.00 87176688.00 56406280.00 84342848.00 63426524.00 49466544.00 61263752.00 64249520.00 85782608.00 70765280.00 96990512.00 66677956.00 D 62243960.00 79464096.00 50593260.00 55021684.00 86470376.00 38662620.00 66551864.00 67397280.00 66603764.00 63313716.00 101127864.00 65567812.00 E 46673716.00 90438744.00 55583492.00 77250440.00 73816912.00 82235664.00 54667044.00 52867872.00 62594524.00 95620640.00 57303524.00 57433164.00 F 87453392.00 74854472.00 55486220.00 90510576.00 48855564.00 43598668.00 56681796.00 52989860.00 64424380.00 65218132.00 61211180.00 62950768.00 G 59245232.00 40295916.00 85735256.00 64902372.00 95145352.00 97243400.00 64180184.00 62030928.00 58073876.00 61721392.00 56960184.00 87595128.00 H 83532280.00 96812200.00 67724400.00 92342008.00 63683068.00 89177376.00 66688468.00 57652564.00 64791104.00 71455704.00 56978260.00 64007088.00 Load time: 04/09/2010 11:14:48 AM Read time: 04/09/2010 11:14:48 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #762 Comment: Plate 26 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 95379808 cps Min counts: 3680983 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 46229048.00 75750528.00 41600080.00 52501996.00 52949012.00 57087836.00 64538576.00 71789888.00 59417852.00 49842504.00 52796140.00 54597792.00 B 64380092.00 70368496.00 65423652.00 64645568.00 60279760.00 67312000.00 81131648.00 75216208.00 56424608.00 70481544.00 58496280.00 44566552.00 C 69646208.00 70013416.00 60083756.00 78864816.00 77103352.00 84060480.00 81382952.00 91383984.00 69325760.00 64542308.00 67150520.00 58872284.00 D 56126668.00 58253360.00 68038224.00 58169224.00 57286492.00 70423832.00 95379808.00 60981196.00 55268280.00 64543664.00 67685792.00 65832944.00 E 53013220.00 48283360.00 64019440.00 62714396.00 64004300.00 70181880.00 65901900.00 62978232.00 62641176.00 65262656.00 66706600.00 64378180.00 F 58122656.00 73362752.00 54507892.00 69062640.00 70440544.00 69928152.00 85041408.00 63917948.00 60000904.00 56397292.00 65798228.00 66216288.00 G 65356404.00 65839308.00 65530456.00 50277800.00 64011760.00 70350888.00 93726904.00 51651036.00 61587040.00 62476912.00 71403728.00 48748160.00 H 61589100.00 68423968.00 58944400.00 65333892.00 72958376.00 66245012.00 81500968.00 60158192.00 54246904.00 67033252.00 55316620.00 54173212.00 Load time: 04/09/2010 11:17:10 AM Read time: 04/09/2010 11:17:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #763 Comment: Rice apricot exp Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 51912320 cps Min counts: 3680983 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44403980.00 44259236.00 45084344.00 47372596.00 47600300.00 45948020.00 45891316.00 46292304.00 46814336.00 44930420.00 42860444.00 44215164.00 B 43058076.00 44587176.00 44823680.00 45333648.00 46579104.00 46505304.00 48308776.00 45422256.00 47562412.00 50785636.00 46127668.00 48651516.00 C 44762360.00 46011316.00 45441040.00 48008676.00 46386168.00 45807820.00 48080664.00 46239908.00 49119728.00 51136844.00 47000480.00 48118120.00 D 44004480.00 47019268.00 45316624.00 46538816.00 46541880.00 45152808.00 46050224.00 48034920.00 49612084.00 46537648.00 48202492.00 48705332.00 E 44804860.00 47249640.00 46164284.00 47887072.00 44657444.00 44446120.00 49085668.00 44098792.00 46124724.00 51912320.00 48076768.00 50993176.00 F 44358444.00 46857240.00 46374948.00 47812556.00 47173344.00 47106160.00 49857352.00 47576552.00 47775816.00 45768972.00 49691584.00 45616456.00 G 43290256.00 46295912.00 46028808.00 48459688.00 47387232.00 49200948.00 50167616.00 48956148.00 50624220.00 45761404.00 46788216.00 49959596.00 H 42683860.00 46361688.00 45671412.00 49720580.00 48100644.00 48439984.00 45084012.00 47725648.00 48574604.00 51498920.00 47023532.00 48959796.00