Load time: 04/29/2010 01:32:10 PM Read time: 04/29/2010 01:32:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #777 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 106922704 cps Min counts: 64109 counts Well list: r0(a6:h8) = cps(f2(a6:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 106922704.00 102719816.00 132819.00 B 64036856.00 62691352.00 135594.00 C 38840260.00 36854336.00 131450.00 D 22371718.00 22002330.00 128218.00 E 12983773.00 12998163.00 248736.00 F 7683482.00 7907582.00 131317.00 G 4920739.00 4677981.00 194875.00 H 2824876.00 2868981.00 139017.00 Load time: 04/29/2010 01:33:58 PM Read time: 04/29/2010 01:33:58 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #778 Comment: plate 32 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37481384 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22603144.00 14601154.00 30012116.00 25360736.00 16703836.00 37481384.00 24399884.00 20318724.00 29894830.00 21377848.00 32167750.00 27375290.00 B 23457632.00 17118162.00 19087688.00 24506232.00 19095596.00 19098546.00 22995542.00 18088806.00 12895984.00 20149174.00 30607218.00 17989730.00 C 28291666.00 18173020.00 36993696.00 13390157.00 21907440.00 35692028.00 8128091.00 18411310.00 27032804.00 25557576.00 36954364.00 16532097.00 D 24271666.00 14020250.00 25572518.00 11389443.00 25501588.00 20823468.00 15941376.00 25953654.00 17221090.00 14077149.00 31945532.00 13723127.00 E 9351779.00 15969720.00 25189370.00 21651620.00 22728688.00 19697368.00 35013688.00 24712000.00 32991564.00 30720472.00 23542020.00 16762258.00 F 14414587.00 20123766.00 19613672.00 12856298.00 25642154.00 14841217.00 22121456.00 20974734.00 23353098.00 33861920.00 31410916.00 11791239.00 G 11615105.00 20457504.00 21805976.00 22925676.00 17673518.00 17299438.00 20940834.00 10717832.00 17979054.00 17907120.00 16369908.00 25443098.00 H 17268710.00 17843726.00 32278282.00 17759896.00 25915220.00 21812086.00 25662606.00 20361064.00 22162252.00 15107951.00 22478722.00 15233377.00 Load time: 04/29/2010 01:36:13 PM Read time: 04/29/2010 01:36:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #779 Comment: plate 33 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40536124 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24952878.00 37346212.00 15368665.00 29147166.00 24869764.00 26133598.00 15771715.00 25961616.00 15012481.00 16631734.00 19227562.00 22903966.00 B 20991980.00 33510022.00 31650154.00 22574708.00 28079580.00 25694866.00 23571734.00 19328068.00 16327931.00 14728322.00 19701952.00 30416418.00 C 25354626.00 34843708.00 37463172.00 37953036.00 21679768.00 39189348.00 14108493.00 18648420.00 17878258.00 17898270.00 27031960.00 15887389.00 D 35000628.00 36731780.00 24069788.00 38720520.00 39876460.00 22852146.00 35556440.00 35143412.00 18566392.00 19175534.00 18111948.00 12769569.00 E 31811118.00 40536124.00 18060950.00 25415530.00 20753212.00 9168911.00 14187532.00 34032840.00 17735344.00 20859614.00 20867326.00 17930580.00 F 29190104.00 10606508.00 21774518.00 34777108.00 23807890.00 12950262.00 15584949.00 21946522.00 17119892.00 17179300.00 21130678.00 23992662.00 G 21662760.00 36101724.00 31440590.00 33061316.00 31978068.00 26885346.00 21248356.00 23021850.00 14541336.00 16343694.00 19482464.00 16538346.00 H 38502088.00 5775246.00 17094038.00 39972872.00 18421500.00 13830217.00 13581345.00 17150206.00 16604605.00 15569215.00 16798056.00 22885052.00 Load time: 04/29/2010 01:38:17 PM Read time: 04/29/2010 01:38:17 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #780 Comment: plate 34 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32788748 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17808548.00 17108874.00 12754887.00 30929974.00 11421866.00 12724396.00 14261739.00 13808563.00 11781910.00 15327314.00 15003052.00 12546694.00 B 15059512.00 21676268.00 24582972.00 11970202.00 22349954.00 14841018.00 18447508.00 14704988.00 12917889.00 17174904.00 14623940.00 14952639.00 C 18027210.00 18893600.00 14715881.00 29982536.00 19517230.00 11533685.00 13961083.00 20184532.00 25594708.00 22585904.00 22011842.00 17624520.00 D 21837162.00 25598828.00 32788748.00 29878888.00 18558736.00 14066876.00 15520870.00 22123780.00 21091644.00 19000040.00 15749513.00 14057887.00 E 19119836.00 21226808.00 16439159.00 22150778.00 11779147.00 14930793.00 12453255.00 14811014.00 19882944.00 16447702.00 17935948.00 17907448.00 F 17334096.00 14797701.00 24810652.00 27999618.00 14048451.00 14257534.00 16434406.00 14168893.00 17297132.00 16566068.00 19696124.00 25845122.00 G 15664356.00 24997572.00 17848796.00 17658930.00 15320258.00 15353514.00 13911924.00 17380986.00 13092195.00 18390482.00 11607695.00 15643463.00 H 18546008.00 15481650.00 20542770.00 17592270.00 14492650.00 10395806.00 16794252.00 12605755.00 17235878.00 20658684.00 21090048.00 15907542.00 Load time: 04/29/2010 01:40:28 PM Read time: 04/29/2010 01:40:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #781 Comment: plate 35 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46594828 cps Min counts: 675028 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12621118.00 15186471.00 14552096.00 13181369.00 15402160.00 1350056.00 14240717.00 14364864.00 13598873.00 12556971.00 14664864.00 21214664.00 B 13982364.00 13114286.00 11730971.00 19485926.00 18475870.00 15394032.00 16290630.00 12149674.00 12101096.00 14500450.00 11367181.00 12929427.00 C 13329010.00 11228796.00 16249534.00 13612232.00 20190602.00 16237946.00 24711296.00 19393820.00 18296546.00 19953608.00 14504473.00 17054618.00 D 11762600.00 12712018.00 16706414.00 9269184.00 14071196.00 17889142.00 14117649.00 19936392.00 16624952.00 17357548.00 15997472.00 20569194.00 E 14915578.00 11963784.00 22238450.00 46594828.00 15843849.00 12616662.00 14551933.00 25377760.00 17552500.00 13008825.00 17619694.00 19235830.00 F 13397183.00 12535586.00 11441182.00 14025200.00 20594108.00 21105366.00 16905048.00 14152568.00 14640849.00 14120106.00 14697080.00 16108161.00 G 12968897.00 12005449.00 11988815.00 13992263.00 20474876.00 18482052.00 23941252.00 15338996.00 14839859.00 11997218.00 13435066.00 18560682.00 H 19887110.00 12883588.00 14238191.00 18158614.00 16486595.00 16276944.00 20837398.00 21813858.00 14914294.00 10258720.00 18971756.00 21631650.00 Load time: 04/29/2010 01:42:33 PM Read time: 04/29/2010 01:42:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #782 Comment: plate 36 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32576014 cps Min counts: 264041 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27001780.00 17317866.00 14343935.00 25845208.00 19861494.00 18970926.00 18181890.00 12038503.00 622231.00 10324740.00 8398539.00 16027633.00 B 20888542.00 17993970.00 14883566.00 14170931.00 21759184.00 17436130.00 18164842.00 15025138.00 528082.00 15503627.00 13892689.00 12491430.00 C 16542105.00 21325630.00 16122603.00 27250662.00 24932986.00 16377687.00 20970836.00 10619222.00 10296387.00 13570743.00 15422324.00 13298396.00 D 21559128.00 19279882.00 25227968.00 32576014.00 15351151.00 16086135.00 22563002.00 14642170.00 16308816.00 10897252.00 9902990.00 15156942.00 E 16688548.00 18042080.00 17154814.00 24020562.00 21422602.00 18135820.00 17275260.00 20507390.00 10678268.00 10332933.00 13506392.00 11661233.00 F 11618832.00 13407055.00 19736108.00 27529354.00 14818679.00 29048228.00 11330515.00 13872396.00 13071822.00 12067865.00 16273305.00 18143172.00 G 20412406.00 18143848.00 10476743.00 17013632.00 22326310.00 21248700.00 13395707.00 15876926.00 9172324.00 13337662.00 11985567.00 15735955.00 H 14597021.00 14308487.00 16058436.00 16524589.00 12856935.00 18166070.00 12512604.00 15537282.00 11066433.00 12357981.00 17444528.00 17858580.00 Load time: 04/29/2010 01:44:37 PM Read time: 04/29/2010 01:44:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #783 Comment: plate 37 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35830960 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11964188.00 17766176.00 21262800.00 13774490.00 15097262.00 11612871.00 16573015.00 10081304.00 12167461.00 15254474.00 15075517.00 16689622.00 B 12678736.00 35830960.00 16317394.00 18487886.00 16817864.00 13631483.00 15419941.00 15627390.00 12415908.00 16642611.00 12307454.00 15052903.00 C 13251529.00 16676184.00 19595418.00 16949714.00 16445097.00 10228497.00 11645015.00 16630742.00 14397051.00 15549133.00 17538386.00 18452970.00 D 15356238.00 21283894.00 16259508.00 17034920.00 14616282.00 10135204.00 16104098.00 21940526.00 20541092.00 20224214.00 12890241.00 13863406.00 E 15376401.00 33868840.00 15652675.00 13290181.00 15167746.00 13692242.00 14323329.00 26406092.00 14214465.00 19490656.00 19950692.00 16863096.00 F 15715822.00 22977492.00 16326699.00 12105347.00 13219586.00 13631507.00 10345122.00 20242982.00 14082012.00 17909838.00 17248134.00 16506409.00 G 10703645.00 17946240.00 18502032.00 13107554.00 16464391.00 17695448.00 12601512.00 21020140.00 20555242.00 10509332.00 13834664.00 22263502.00 H 13022550.00 18261234.00 14235537.00 18284132.00 16088662.00 12849934.00 14972130.00 18472056.00 19224904.00 18930802.00 17768202.00 15012047.00 Load time: 04/29/2010 01:46:39 PM Read time: 04/29/2010 01:46:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #784 Comment: plate 38 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31617644 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15942356.00 18076378.00 18456820.00 13400319.00 14719747.00 15701816.00 21122424.00 20446008.00 19795208.00 14879101.00 16643999.00 16995586.00 B 14079108.00 20639248.00 23173320.00 21002450.00 14933313.00 20463686.00 18951214.00 15512521.00 24222518.00 25880598.00 17159618.00 11941330.00 C 16340366.00 15328792.00 17499408.00 13683197.00 21989344.00 18451224.00 17700836.00 31617644.00 21853218.00 16646132.00 21294080.00 13546804.00 D 20431686.00 15837115.00 18317764.00 15568842.00 21443840.00 16479619.00 21304916.00 15490827.00 26473958.00 23364028.00 16874934.00 14435322.00 E 15322850.00 17498622.00 17057120.00 17783458.00 15523653.00 19614418.00 21351178.00 17430470.00 18256012.00 23922862.00 15599311.00 16990544.00 F 19466928.00 19111154.00 16933276.00 17021550.00 15798751.00 23626084.00 17492648.00 22852950.00 16306971.00 23348966.00 16466655.00 16969994.00 G 14255447.00 15259825.00 14642748.00 18738660.00 15506406.00 15428557.00 16892834.00 18807812.00 21007550.00 15401216.00 16280822.00 20825250.00 H 18636104.00 13069717.00 22667558.00 17886596.00 15457128.00 17351708.00 16415041.00 18719832.00 22004878.00 16957062.00 18478062.00 20258674.00 Load time: 04/29/2010 01:48:37 PM Read time: 04/29/2010 01:48:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #785 Comment: plate 39 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29377412 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17543128.00 19387302.00 21579740.00 16799652.00 17945330.00 17098468.00 15138027.00 15543544.00 15417743.00 17838002.00 16095143.00 17667864.00 B 13473699.00 15596374.00 25405330.00 17080802.00 20699208.00 15808212.00 21556862.00 13208914.00 15795579.00 13681176.00 17873500.00 23141780.00 C 15927714.00 14560993.00 14962954.00 20364950.00 15490026.00 14953731.00 19638506.00 13967518.00 14707738.00 11960420.00 16515440.00 17292178.00 D 10115089.00 18989938.00 18176542.00 19343912.00 19176712.00 22861000.00 13692555.00 20271176.00 19397490.00 15646983.00 17524690.00 22279104.00 E 18070590.00 15804492.00 19703448.00 17607146.00 22590818.00 29377412.00 18917972.00 17458330.00 15368785.00 14829856.00 21624022.00 12149484.00 F 14352633.00 16287701.00 26060572.00 13366990.00 20368626.00 18163018.00 18199608.00 17112818.00 14144574.00 20125220.00 15234838.00 13728788.00 G 14090597.00 23759802.00 20473424.00 15942257.00 18083766.00 24329372.00 19380884.00 18022160.00 20237038.00 26626848.00 14231973.00 14215683.00 H 12964254.00 26811662.00 21709140.00 15353605.00 16914260.00 16276772.00 10494363.00 17339614.00 11830612.00 11907330.00 15981878.00 17561684.00 Load time: 04/29/2010 01:50:51 PM Read time: 04/29/2010 01:50:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #786 Comment: 318 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40487644 cps Min counts: 778739 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29862502.00 24606818.00 20876782.00 16841578.00 14022194.00 16312369.00 20601136.00 19023384.00 18433100.00 32263172.00 32484698.00 31020282.00 B 33543796.00 20851292.00 19333488.00 14327573.00 12120797.00 16664870.00 19670502.00 16829022.00 16765435.00 33407524.00 27628100.00 34299808.00 C 29051046.00 26891938.00 31063964.00 14339393.00 21908798.00 15771267.00 18308462.00 21759242.00 13162877.00 33262404.00 31869966.00 37033624.00 D 33210484.00 34230004.00 40487644.00 15465693.00 13471951.00 15132937.00 16688620.00 12343956.00 18866548.00 35128040.00 36377796.00 36725196.00 E 33812076.00 33468256.00 33623168.00 17749732.00 12408766.00 16484956.00 20921372.00 14415788.00 14009658.00 29731050.00 35428648.00 35133264.00 F 29889554.00 27391080.00 30582216.00 13863876.00 11745233.00 23992956.00 19077586.00 14691590.00 14130355.00 27568728.00 36420156.00 32688276.00 G 33699700.00 28544072.00 30270990.00 19288952.00 13642086.00 21446554.00 19905206.00 15153608.00 18383622.00 34268868.00 35398852.00 34929976.00 H 33053596.00 32864030.00 32522548.00 21473404.00 20755150.00 22537404.00 21520164.00 21901342.00 21503882.00 32140536.00 36041404.00 37302204.00 Load time: 04/29/2010 01:52:34 PM Read time: 04/29/2010 01:52:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #787 Comment: 318 (sensitivity 1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 41623896 cps Min counts: 3679993 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 30472276.00 25319828.00 21601456.00 17247620.00 14184477.00 16364181.00 20781074.00 19402032.00 18770382.00 32331296.00 32973346.00 30927280.00 B 34454416.00 21141358.00 19922786.00 14487627.00 12167842.00 16725283.00 19820920.00 16818154.00 16921846.00 33750200.00 27970428.00 34836548.00 C 29708490.00 27488732.00 31876180.00 14663834.00 21928884.00 16109708.00 18386326.00 21940324.00 13441278.00 33111276.00 31842696.00 37560272.00 D 33167826.00 34675736.00 41623896.00 15858535.00 13597671.00 15306029.00 16747752.00 12359257.00 19023748.00 36011428.00 37130020.00 37189156.00 E 35252728.00 33849508.00 34663224.00 18183970.00 12468886.00 16339623.00 21139864.00 14635393.00 14174877.00 30013348.00 36019040.00 35766568.00 F 30568388.00 28086064.00 31714348.00 14034379.00 12003747.00 24050736.00 19314692.00 14639481.00 14205853.00 28060838.00 37000364.00 32894648.00 G 34939380.00 29896696.00 31328240.00 19812532.00 13815959.00 21951814.00 20098130.00 15496667.00 18546364.00 34648212.00 36065356.00 36061220.00 H 33800360.00 33872320.00 33326966.00 22048194.00 21069690.00 23007422.00 22022506.00 22228062.00 21731210.00 32548024.00 36482864.00 37830556.00 Load time: 04/29/2010 01:54:55 PM Read time: 04/29/2010 01:54:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #788 Comment: 319 (sensitivity 1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 65190556 cps Min counts: 3679993 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27863418.00 29305052.00 28901932.00 20082916.00 18575666.00 20997950.00 44441952.00 54787412.00 50873888.00 26186778.00 27762994.00 24414602.00 B 32055026.00 27605570.00 28032772.00 18564806.00 19483422.00 20986154.00 52964636.00 49779288.00 49091452.00 33425954.00 28887210.00 22422130.00 C 32721844.00 31467836.00 33495624.00 19232756.00 20787262.00 18508102.00 48128020.00 49777604.00 61026464.00 35838776.00 27482862.00 26999160.00 D 20898022.00 34856212.00 35150332.00 20711542.00 26001050.00 18411338.00 56744580.00 49032000.00 62040488.00 17528178.00 21240158.00 31732424.00 E 26784004.00 34456128.00 33452754.00 17296918.00 17170428.00 25169992.00 65190556.00 52622612.00 58567756.00 30170808.00 30371150.00 29002658.00 F 32079362.00 29392992.00 27193904.00 20276018.00 17623484.00 20376204.00 50697700.00 51446852.00 58352024.00 35380540.00 30709530.00 26007004.00 G 32104242.00 27429130.00 28705726.00 19811414.00 15628530.00 18042280.00 53177412.00 54785696.00 59871464.00 33708492.00 33708804.00 29902116.00 H 30627362.00 28788004.00 28553220.00 18208920.00 19557644.00 19876538.00 58978136.00 54587488.00 39323524.00 33481454.00 30082264.00 26174410.00 Load time: 04/29/2010 01:57:21 PM Read time: 04/29/2010 01:57:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #789 Comment: 321 (sensitivity 1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 52258828 cps Min counts: 3679993 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44127852.00 41628136.00 41872212.00 14178941.00 17581202.00 31349138.00 35785660.00 35534824.00 43242352.00 35510276.00 34869260.00 30938358.00 B 43716292.00 41375208.00 34135304.00 14994647.00 16944012.00 16293510.00 35830296.00 32116428.00 25689544.00 38106732.00 37257644.00 34242628.00 C 42474976.00 43999008.00 41818156.00 15617393.00 20130650.00 32998950.00 36117920.00 42631300.00 45596732.00 38081576.00 40617224.00 38060264.00 D 43330184.00 48728660.00 49723732.00 16214011.00 19310688.00 28268526.00 35533452.00 32089714.00 31465146.00 44770572.00 25349912.00 41897620.00 E 45211484.00 45819508.00 48058188.00 18369530.00 20994044.00 18627964.00 36511132.00 47277656.00 30749764.00 39329324.00 39749420.00 41427928.00 F 41463644.00 42999620.00 52258828.00 16512632.00 17998440.00 21791500.00 39215912.00 32270426.00 31927234.00 42264576.00 41012536.00 36009564.00 G 41868968.00 41121748.00 41120236.00 12329141.00 18329374.00 16996184.00 37076192.00 33637844.00 30148042.00 38774896.00 35515964.00 30315260.00 H 41149936.00 43774840.00 43851004.00 14149734.00 17488396.00 16891326.00 36173520.00 35954956.00 33929188.00 37123316.00 34693684.00 35552816.00 Load time: 04/29/2010 01:59:49 PM Read time: 04/29/2010 01:59:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #790 Comment: standard (sensitivity 1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 113572336 cps Min counts: 67113 counts Well list: r0(a6:h8) = cps(f2(a6:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 113572336.00 111454160.00 140964.00 B 67822600.00 66789120.00 142581.00 C 41457896.00 39937420.00 136921.00 D 23746750.00 23353650.00 134226.00 E 13885418.00 13976837.00 265308.00 F 8357242.00 8381083.00 142761.00 G 5282804.00 4988836.00 209335.00 H 3053155.00 3046237.00 146355.00 Load time: 04/29/2010 02:00:50 PM Read time: 04/29/2010 02:00:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #791 Comment: standard (sensitivity 2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 112819912 cps Min counts: 67930 counts Well list: r0(a6:h8) = cps(f2(a6:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 112819912.00 109738792.00 141013.00 B 67762432.00 66869260.00 144169.00 C 41420332.00 40097804.00 139815.00 D 23783748.00 23185186.00 135861.00 E 13910880.00 13819884.00 264041.00 F 8250285.00 8392167.00 143218.00 G 5223130.00 4955934.00 207822.00 H 3022600.00 3026374.00 145576.00