Load time: 04/30/2010 02:26:31 PM Read time: 04/30/2010 02:26:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #795 Comment: standard (sensitivity 1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 81735072 cps Min counts: 125294 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19180108.00 26226584.00 79702768.00 81735072.00 253284.00 B 66184804.00 26035738.00 47467104.00 47101684.00 250589.00 C 41008932.00 39887448.00 28532526.00 28119252.00 259035.00 D 41036868.00 33422390.00 16811256.00 16805530.00 254273.00 E 38355876.00 34943248.00 10128198.00 10137904.00 254722.00 F 36190752.00 29465256.00 6023125.00 5937409.00 258226.00 G 42081968.00 21319330.00 3602500.00 3638350.00 256788.00 H 43801656.00 24458096.00 2281179.00 2358000.00 264066.00 Load time: 04/30/2010 02:27:46 PM Read time: 04/30/2010 02:27:46 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #796 Comment: standard (sensitivity 2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 83271376 cps Min counts: 131530 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20413424.00 27337916.00 82866320.00 83271376.00 263060.00 B 69414960.00 27080786.00 49051616.00 48325444.00 263592.00 C 42965168.00 41435588.00 29903606.00 28859408.00 271575.00 D 42940524.00 34934860.00 17347246.00 17292072.00 263782.00 E 40274248.00 36317124.00 10408812.00 10379322.00 264524.00 F 37133508.00 31004704.00 6227323.00 6118725.00 265721.00 G 44405640.00 22600630.00 3713913.00 3718178.00 268553.00 H 45785004.00 25573608.00 2338162.00 2421559.00 274483.00 Load time: 04/30/2010 02:29:41 PM Read time: 04/30/2010 02:29:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #797 Comment: standard (sensitivity 2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31550390 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15590111.00 11031192.00 9850976.00 13349934.00 16357787.00 10566297.00 24732434.00 17889558.00 12935481.00 10422105.00 18923732.00 15233717.00 B 13213960.00 8327770.00 9884519.00 13776863.00 20819572.00 12295595.00 21038650.00 8542533.00 12324657.00 14192876.00 23528798.00 13991248.00 C 11924061.00 9167816.00 17910836.00 17997120.00 23398614.00 13936050.00 20194580.00 15572585.00 20148016.00 12575837.00 13217391.00 15417956.00 D 10535127.00 11709354.00 11179650.00 12679308.00 16400551.00 15651635.00 13129679.00 16402623.00 17282856.00 15391803.00 15739508.00 16273639.00 E 17003868.00 11860717.00 28307776.00 16099455.00 13428407.00 13209790.00 10977638.00 11327657.00 16052152.00 14753353.00 13067269.00 14614656.00 F 14618362.00 7879511.00 23882688.00 12796977.00 10512153.00 11092599.00 12507048.00 14172439.00 31544766.00 18783566.00 28520988.00 19860548.00 G 11553846.00 14842345.00 19774790.00 9596042.00 11892041.00 31550390.00 10914493.00 21517556.00 19930916.00 25250624.00 18556088.00 20287356.00 H 11772156.00 9783595.00 18694664.00 14747568.00 21916802.00 11297592.00 15880869.00 14379785.00 9852198.00 16262897.00 18194716.00 13597149.00 Load time: 04/30/2010 02:32:13 PM Read time: 04/30/2010 02:32:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #798 Comment: plate 45 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32492402 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11228213.00 16158733.00 16665326.00 24571552.00 17856362.00 12921590.00 10730781.00 11505746.00 13625158.00 22966246.00 15761877.00 13434154.00 B 14926464.00 14272336.00 14695957.00 24219624.00 17882654.00 14184653.00 20453916.00 13137168.00 16089805.00 17143680.00 14279169.00 13201500.00 C 29108336.00 17598440.00 16678036.00 23644850.00 15495995.00 16068122.00 14548674.00 18906312.00 18681968.00 19006056.00 16164604.00 11277480.00 D 32492402.00 19165552.00 24395086.00 26653386.00 18388186.00 14227188.00 15510104.00 19904672.00 14913396.00 19531648.00 22578998.00 14365112.00 E 21844116.00 24426848.00 29199604.00 17302408.00 17513956.00 15241412.00 20562664.00 15285280.00 13305375.00 16134421.00 15000577.00 14724863.00 F 26411602.00 24375762.00 22456492.00 18811250.00 16104583.00 16596195.00 22908198.00 18107880.00 14077110.00 19001000.00 14439446.00 17203004.00 G 23855468.00 20745564.00 30055648.00 17580518.00 16148610.00 14291304.00 15513071.00 14109178.00 17948908.00 17174124.00 14980055.00 18117320.00 H 15456740.00 23725408.00 22195224.00 18981958.00 11001481.00 15646540.00 11229754.00 14782009.00 19076236.00 15965577.00 17349216.00 16586930.00 Load time: 04/30/2010 02:34:15 PM Read time: 04/30/2010 02:34:15 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #799 Comment: plate 46 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37665820 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21355948.00 16542381.00 16607876.00 14826686.00 12893835.00 16200965.00 17329136.00 18402486.00 24102354.00 21416868.00 17273158.00 14743157.00 B 19970896.00 16540201.00 19482480.00 16509196.00 18937448.00 19249522.00 31792220.00 30003408.00 29487038.00 37665820.00 16589688.00 17150590.00 C 15373081.00 19262290.00 18501628.00 20472526.00 24048230.00 21471030.00 18981960.00 15429324.00 23555710.00 26355986.00 20728110.00 14273986.00 D 18896814.00 12534998.00 14376624.00 14811200.00 20085276.00 18011776.00 21169656.00 22259700.00 23647150.00 23617164.00 19425896.00 14732693.00 E 16566707.00 15791130.00 15978094.00 23034808.00 25235484.00 20553054.00 24850536.00 23010164.00 21420698.00 14817796.00 16174275.00 13404708.00 F 16930284.00 17349448.00 17480412.00 15023182.00 18206544.00 32752804.00 29601396.00 29361486.00 17432738.00 29654500.00 14341694.00 15884207.00 G 20302064.00 20028284.00 22215174.00 17333844.00 20360574.00 21803312.00 25353496.00 25330232.00 21383692.00 26548588.00 16190656.00 16751997.00 H 15915737.00 17171322.00 17960334.00 17290382.00 19399030.00 19423860.00 24960244.00 14504306.00 21708654.00 15568319.00 14732554.00 17209240.00 Load time: 04/30/2010 02:37:53 PM Read time: 04/30/2010 02:37:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #800 Comment: plate 47 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27595870 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16255290.00 12438468.00 17150364.00 16053277.00 21764972.00 17543044.00 18587942.00 16607947.00 17357144.00 19803814.00 15563309.00 17872800.00 B 14181113.00 17676034.00 19454244.00 15637740.00 15211543.00 16227034.00 15945498.00 17125390.00 19588310.00 21942066.00 14227475.00 14598571.00 C 16668679.00 15219573.00 20650642.00 20853642.00 24609846.00 19487406.00 18384106.00 16355038.00 23262210.00 20765372.00 18921800.00 25490882.00 D 13947134.00 18616792.00 14568113.00 17119592.00 18257656.00 23284336.00 17135570.00 18797442.00 14471870.00 26124188.00 19917606.00 17210458.00 E 12587591.00 15839996.00 18532456.00 23349562.00 16752828.00 18666872.00 16125864.00 17585324.00 20110386.00 26630958.00 25749882.00 21696252.00 F 14550278.00 18428098.00 20541614.00 14663628.00 23139904.00 23219130.00 20294126.00 18863846.00 17986144.00 22786572.00 21405972.00 21893506.00 G 17684184.00 14880614.00 18214426.00 18633364.00 24451628.00 18934410.00 15536629.00 15416002.00 16213213.00 15344931.00 20958286.00 20094814.00 H 27595870.00 16280955.00 16132915.00 14313587.00 17664644.00 16214495.00 16070112.00 20392038.00 14888925.00 21740964.00 14328919.00 19052054.00 Load time: 04/30/2010 02:39:57 PM Read time: 04/30/2010 02:39:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #801 Comment: plate 48 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32542516 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16913990.00 17060280.00 26511434.00 18305176.00 19368240.00 18605090.00 17923702.00 16860584.00 12226064.00 14423048.00 16541644.00 15312277.00 B 17881094.00 15417682.00 12636112.00 15936719.00 17181970.00 22028488.00 27887774.00 16982060.00 14640904.00 16044676.00 14144553.00 13191615.00 C 24271348.00 21869950.00 23219682.00 22857822.00 20262962.00 17880312.00 26548500.00 17250574.00 11534862.00 16924542.00 13637640.00 15224989.00 D 20942444.00 15598045.00 11579072.00 22128418.00 18964480.00 22663458.00 22862050.00 16682792.00 12144685.00 18199440.00 17463162.00 13890696.00 E 19262384.00 19558000.00 17395890.00 19073948.00 24649506.00 32542516.00 26214088.00 19788008.00 11853079.00 18091764.00 13595249.00 16530405.00 F 17347902.00 22437724.00 19281180.00 17104322.00 14244449.00 20746778.00 25102124.00 21703752.00 19437098.00 17847684.00 11079749.00 13751458.00 G 21820056.00 18579028.00 17036352.00 18804434.00 16497266.00 20222170.00 26643078.00 13308605.00 13179511.00 16517077.00 13578746.00 16186214.00 H 23291164.00 25841078.00 21043656.00 16425707.00 20960768.00 20363236.00 21770754.00 11271422.00 12072500.00 16286713.00 15956283.00 18040408.00 Load time: 04/30/2010 02:42:31 PM Read time: 04/30/2010 02:42:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #802 Comment: plate 49 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35902116 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12386993.00 14466142.00 27352614.00 18821574.00 9928640.00 11007236.00 9266835.00 10399912.00 10162878.00 15236282.00 7587632.00 12941717.00 B 14393957.00 18011486.00 18060874.00 10471504.00 14901692.00 9103061.00 11108031.00 7524921.00 13636159.00 8978220.00 9264101.00 9647842.00 C 14570429.00 26809510.00 17233124.00 12124503.00 17218412.00 8484062.00 11116769.00 10772980.00 12107440.00 10169953.00 16311827.00 13040915.00 D 12378761.00 26210910.00 15319419.00 16996630.00 9680111.00 9207685.00 10381094.00 14528205.00 11763173.00 13178105.00 8966319.00 14490913.00 E 13809706.00 18489280.00 21897262.00 35902116.00 18534708.00 11632444.00 9716740.00 11574941.00 12925644.00 11432570.00 7171884.00 15625719.00 F 20800714.00 21560042.00 15316434.00 17758082.00 12439801.00 11575939.00 20543998.00 9351531.00 15885568.00 11400276.00 11017906.00 8828827.00 G 15960962.00 16986430.00 20972796.00 23940118.00 11256478.00 11202493.00 12715570.00 11887828.00 14571739.00 11194278.00 9596917.00 12358795.00 H 16611845.00 23526954.00 16674001.00 9950733.00 11617499.00 16248710.00 9810384.00 8905286.00 12808051.00 9628714.00 9060093.00 9067511.00 Load time: 04/30/2010 02:44:39 PM Read time: 04/30/2010 02:44:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #803 Comment: plate 50 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16651603 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12647423.00 5550574.00 9752698.00 9525933.00 8353764.00 8899461.00 5737292.00 7971687.00 8638399.00 9963595.00 7960554.00 6869372.00 B 7673653.00 5490689.00 5695959.00 9470589.00 9734505.00 9051898.00 9043108.00 10061496.00 7750937.00 11317168.00 8061196.00 5323547.00 C 10481555.00 9626096.00 7725110.00 9596065.00 10622522.00 9496559.00 8091415.00 9381245.00 13972140.00 7248095.00 8893627.00 5714669.00 D 7093127.00 8945673.00 9154601.00 11761272.00 13894712.00 11983768.00 5171082.00 12266632.00 11265943.00 12526648.00 9518725.00 10443382.00 E 11404903.00 7189680.00 10440749.00 11970538.00 10030024.00 9873413.00 7799093.00 10070645.00 12011223.00 10483051.00 11728864.00 14833946.00 F 6510310.00 6114112.00 15465521.00 12019381.00 11699096.00 7753584.00 5345548.00 14858832.00 9277901.00 11156708.00 9497022.00 16035125.00 G 6996975.00 7922754.00 8455635.00 8637987.00 8460818.00 6614771.00 7032191.00 9479493.00 14987899.00 10285421.00 8325900.00 11016566.00 H 5025091.00 11575508.00 10612849.00 16651603.00 7695841.00 7744191.00 8053366.00 7936096.00 12090160.00 9042110.00 5178984.00 11540658.00 Load time: 04/30/2010 02:46:52 PM Read time: 04/30/2010 02:46:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #804 Comment: plate 43 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23375432 cps Min counts: 778537 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9676622.00 9573633.00 14440330.00 15126012.00 11046579.00 18254614.00 12729894.00 6140611.00 15487949.00 10252992.00 14316533.00 10741026.00 B 10210969.00 13492989.00 15067289.00 9872499.00 15939917.00 13079629.00 15542399.00 11153191.00 10313270.00 13351582.00 11883654.00 13393594.00 C 8376260.00 13165403.00 18408272.00 14472866.00 23302874.00 15494392.00 13211515.00 10368816.00 13700807.00 14853730.00 13166049.00 13921023.00 D 10933270.00 12567008.00 17546680.00 14298654.00 14989140.00 15836195.00 17994998.00 12660112.00 16865294.00 20865270.00 14722052.00 14789031.00 E 12886428.00 12503271.00 15227967.00 10651589.00 15024487.00 10323458.00 23375432.00 12784034.00 17183638.00 14028991.00 15969704.00 15695149.00 F 15503031.00 14189720.00 16709035.00 10542487.00 16183385.00 10228812.00 21448494.00 19212710.00 21322022.00 14618471.00 14517259.00 15875462.00 G 15201296.00 12552946.00 14828692.00 14934938.00 13448471.00 17378648.00 15575171.00 17681574.00 9262524.00 13453190.00 22082910.00 13860551.00 H 12579780.00 17221880.00 17677960.00 14362701.00 9561218.00 13904317.00 13171528.00 14803146.00 12730622.00 12286747.00 11938378.00 12569665.00 Load time: 04/30/2010 02:49:22 PM Read time: 04/30/2010 02:49:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #805 Comment: Standard (sensitivity 2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 86065224 cps Min counts: 137660 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20610350.00 27700340.00 86065224.00 85588360.00 277658.00 B 70563792.00 26648458.00 51088820.00 50393056.00 275985.00 C 41574548.00 41551028.00 30685268.00 30199864.00 284633.00 D 41390208.00 34775076.00 18064352.00 17926258.00 275643.00 E 38363500.00 36293080.00 10933026.00 10786802.00 275320.00 F 36505120.00 31343978.00 6465951.00 6299422.00 277639.00 G 42398920.00 21778428.00 3882780.00 3853050.00 281839.00 H 43583308.00 25749202.00 2433407.00 2522625.00 287161.00 Load time: 04/30/2010 02:50:43 PM Read time: 04/30/2010 02:50:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #806 Comment: Standard (sensitivity 1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 87196176 cps Min counts: 136974 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21436692.00 29041998.00 87196176.00 86481280.00 277184.00 B 72695832.00 28572198.00 51035620.00 50516176.00 273949.00 C 45003772.00 42891048.00 30783318.00 30156118.00 283473.00 D 44685472.00 35799172.00 18161348.00 17984442.00 276555.00 E 41545108.00 37484452.00 10885366.00 10824467.00 276465.00 F 38816172.00 32255520.00 6506334.00 6345414.00 276735.00 G 45995616.00 23056330.00 3892263.00 3886513.00 282485.00 H 46795932.00 26501968.00 2439493.00 2533745.00 282575.00