Load time: 05/03/2010 02:04:05 PM Read time: 05/03/2010 02:04:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #818 Comment: Standard (2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 108977584 cps Min counts: 68515 counts Well list: r0(a6:h8) = cps(f2(a6:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 108977584.00 108386256.00 215446.00 B 63723656.00 63886188.00 141481.00 C 37555036.00 37554672.00 166510.00 D 22320592.00 21932542.00 202361.00 E 13115731.00 13373816.00 137031.00 F 8068401.00 7828758.00 262156.00 G 4503465.00 4417387.00 175468.00 H 2827988.00 2851986.00 142699.00 Load time: 05/03/2010 02:06:03 PM Read time: 05/03/2010 02:06:03 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #820 Comment: Plate 54 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41464116 cps Min counts: 779015 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23039818.00 13457705.00 14276755.00 12447060.00 12441434.00 18211250.00 12994440.00 18609576.00 18071164.00 16906778.00 20438606.00 19815418.00 B 14168913.00 19218956.00 24596422.00 12420071.00 23382058.00 24377214.00 13154312.00 28616600.00 26490904.00 17759302.00 40710904.00 7587056.00 C 14127877.00 16568485.00 19063190.00 19354420.00 19396732.00 23222720.00 10248423.00 36532844.00 33519304.00 23623564.00 18243192.00 17494286.00 D 17485570.00 14234468.00 20277354.00 16780012.00 18719582.00 36096948.00 9793835.00 17776242.00 23727320.00 13788225.00 13948675.00 23459232.00 E 10314164.00 26252986.00 22123952.00 19246780.00 17994276.00 19003064.00 20523250.00 18798092.00 23274478.00 41464116.00 18730430.00 24864996.00 F 14430326.00 34482672.00 23836074.00 21745932.00 24556104.00 27410432.00 16556866.00 24295942.00 19442720.00 34734052.00 13877040.00 33118868.00 G 11232840.00 34382384.00 11097164.00 16215299.00 22366926.00 7825651.00 18723900.00 12060798.00 22096908.00 17009850.00 4439056.00 16741317.00 H 13443492.00 16485285.00 18051442.00 15888869.00 22443674.00 22290828.00 12437004.00 27865566.00 16574019.00 37888376.00 14703120.00 16800784.00 Load time: 05/03/2010 02:08:02 PM Read time: 05/03/2010 02:08:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #821 Comment: Plate 56 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42157700 cps Min counts: 779015 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18252126.00 26314086.00 17591948.00 32499192.00 21575250.00 26797916.00 19567550.00 27152854.00 26307688.00 26477848.00 31590882.00 7331771.00 B 19980084.00 22516198.00 24732690.00 15689854.00 10998600.00 24317634.00 12849039.00 14706782.00 22987198.00 25400082.00 32568896.00 16494745.00 C 23168710.00 23507228.00 24069686.00 29048228.00 19838124.00 22114344.00 18105144.00 23742580.00 18076406.00 26086482.00 31133840.00 25398424.00 D 28508736.00 25314486.00 28065648.00 27963796.00 27660954.00 27439878.00 12854636.00 23656708.00 28241054.00 22858566.00 34538172.00 11359139.00 E 25002982.00 21978952.00 28055540.00 38352088.00 23955406.00 26674316.00 22675262.00 26201508.00 21723798.00 16236964.00 23429596.00 28055642.00 F 24020112.00 20074302.00 8659451.00 33668368.00 21354474.00 2063851.00 15678140.00 27150488.00 14415933.00 33289692.00 42157700.00 19951936.00 G 26470652.00 21383016.00 27654276.00 18470146.00 15468747.00 27900498.00 18716928.00 35883120.00 24423226.00 28207924.00 32480492.00 25163622.00 H 22501630.00 16770295.00 31253002.00 14569396.00 20787826.00 21519702.00 18867200.00 24915656.00 6387115.00 25491592.00 31074974.00 22295612.00 Load time: 05/03/2010 02:10:02 PM Read time: 05/03/2010 02:10:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #822 Comment: Plate 57 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 34338428 cps Min counts: 779015 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6845784.00 19203226.00 23494394.00 24061508.00 23868502.00 11715927.00 19577038.00 27348844.00 19278982.00 29461402.00 15916951.00 20715632.00 B 20562364.00 13149870.00 12914668.00 23340866.00 29027772.00 18236914.00 27245160.00 23148742.00 19145840.00 27074338.00 16849696.00 6280503.00 C 25494926.00 13665833.00 25719840.00 11928768.00 18008168.00 18889756.00 20765548.00 34185336.00 18115332.00 22036214.00 24339214.00 27978054.00 D 28996220.00 26200362.00 9484214.00 32902474.00 19545160.00 11297593.00 20985560.00 24871666.00 24682458.00 15156796.00 15011868.00 25252614.00 E 18542996.00 19743602.00 26188736.00 23173946.00 20242578.00 20085118.00 22244490.00 21183172.00 24252830.00 16032786.00 19483910.00 25176470.00 F 34338428.00 19896746.00 11514647.00 11479760.00 17179060.00 26343274.00 21976968.00 27494984.00 27073210.00 19826312.00 21986644.00 21959686.00 G 23817492.00 15898565.00 14747042.00 21284398.00 21441046.00 20669296.00 14593823.00 22466780.00 30350734.00 19381382.00 16976710.00 16452452.00 H 27042478.00 20558944.00 7474161.00 19975624.00 22875280.00 31947678.00 15077737.00 17364124.00 17764568.00 22280310.00 18297914.00 15011579.00 Load time: 05/03/2010 02:20:44 PM Read time: 05/03/2010 02:20:44 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #823 Comment: Plate 58 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 39883032 cps Min counts: 65139 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18122242.00 17182888.00 24264688.00 20633164.00 18801448.00 34775608.00 505390.00 31846592.00 34181584.00 28365276.00 29715040.00 21412522.00 B 23954374.00 133665.00 30516832.00 12547260.00 12454163.00 25019686.00 27170280.00 16758967.00 12637901.00 39883032.00 28298506.00 25929582.00 C 20504236.00 22422742.00 27032250.00 16535356.00 25040676.00 31875912.00 31148406.00 22875348.00 18206526.00 33778444.00 28528154.00 15030143.00 D 19219098.00 22009132.00 25734114.00 27091474.00 21245800.00 21610800.00 12562029.00 24790146.00 298388.00 20533042.00 24649892.00 29193116.00 E 18506918.00 25635708.00 21741198.00 16365216.00 26101078.00 25325186.00 23997246.00 147282.00 32082540.00 31569248.00 32505838.00 22442832.00 F 29001836.00 10370390.00 130279.00 16421411.00 22542062.00 12097959.00 17457124.00 24974606.00 25633432.00 19649726.00 24515070.00 21206930.00 G 17662104.00 27197980.00 29125012.00 14215403.00 26643208.00 17495900.00 26346302.00 25001378.00 17915484.00 33002832.00 27350766.00 13385351.00 H 21032812.00 25602178.00 30768380.00 13920112.00 25826084.00 14637863.00 17978660.00 33083142.00 26777926.00 5050896.00 21933776.00 7574478.00 Load time: 05/03/2010 02:22:41 PM Read time: 05/03/2010 02:22:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #824 Comment: Plate 65 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38296660 cps Min counts: 779015 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11023033.00 27515476.00 25559088.00 25607986.00 11818934.00 23051954.00 27077354.00 20682740.00 10877476.00 21083986.00 20479172.00 14707561.00 B 17594212.00 12193683.00 9922221.00 12622686.00 38220944.00 38296660.00 27557916.00 16572503.00 20424138.00 24339666.00 27358066.00 24455656.00 C 18025714.00 17125206.00 18124982.00 19600190.00 15169459.00 20830292.00 15192534.00 20463192.00 24672140.00 26376098.00 25438150.00 15992434.00 D 7965351.00 16443909.00 14124189.00 23447228.00 10068870.00 11161974.00 23923184.00 19759928.00 24681442.00 19967432.00 14102889.00 16429934.00 E 22862458.00 18405996.00 7266331.00 28539440.00 36514864.00 13089286.00 22537044.00 19254298.00 23809704.00 14415480.00 16137204.00 18263040.00 F 17960546.00 21741744.00 14657556.00 32847950.00 17528298.00 21118512.00 11852434.00 21332016.00 17237410.00 35375540.00 21211146.00 16448492.00 G 20086932.00 14645238.00 16831494.00 20394088.00 24950448.00 17996436.00 18791786.00 12353029.00 21350960.00 24323784.00 25838332.00 20455562.00 H 15829366.00 14614439.00 9550572.00 30136694.00 36906868.00 25139260.00 16195107.00 16418401.00 16212194.00 27587290.00 19141274.00 5680248.00 Load time: 05/03/2010 02:24:40 PM Read time: 05/03/2010 02:24:40 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #825 Comment: Plate 68 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 69458808 cps Min counts: 779015 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20599336.00 13897836.00 14888015.00 14026584.00 16282609.00 18945248.00 21987450.00 17510212.00 15616538.00 10800413.00 19386304.00 18323782.00 B 21737194.00 16267444.00 13962776.00 23811596.00 23049226.00 12421675.00 12915633.00 21192508.00 17211358.00 11614199.00 11914574.00 23192644.00 C 16152729.00 36012176.00 6237063.00 46266692.00 21006610.00 17324928.00 22031540.00 15687674.00 9597567.00 17574246.00 23384868.00 69458808.00 D 20313416.00 20183158.00 15302537.00 4962893.00 22821736.00 12819584.00 26321378.00 19037614.00 12475184.00 19839942.00 26901560.00 26144440.00 E 21111700.00 10949917.00 15157593.00 15169459.00 16285538.00 20347212.00 13802564.00 16567218.00 7951595.00 16271183.00 22777830.00 32912900.00 F 10484701.00 8428367.00 16532410.00 10179123.00 29350070.00 20526766.00 15827084.00 16495163.00 17277434.00 19934630.00 20152926.00 21047190.00 G 16145294.00 9799712.00 11704502.00 22717382.00 24993194.00 28806012.00 14586064.00 26656244.00 21668932.00 24508976.00 18185998.00 23564540.00 H 18770280.00 9079984.00 6422581.00 32812252.00 29425458.00 20507636.00 19510700.00 22936368.00 9963248.00 20361304.00 24182210.00 24815492.00 Load time: 05/03/2010 02:27:00 PM Read time: 05/03/2010 02:27:00 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #826 Comment: Plate 70 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36456248 cps Min counts: 779015 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15875821.00 13192091.00 15484244.00 31716804.00 27444034.00 21252462.00 21035482.00 18265606.00 15039197.00 22170862.00 32473860.00 12141212.00 B 14713449.00 12877437.00 28724546.00 20546362.00 22154912.00 16304112.00 21473908.00 14778009.00 19240078.00 18564560.00 19042038.00 19720260.00 C 20157932.00 19967380.00 28513324.00 17820730.00 21435738.00 18041742.00 13205688.00 20436890.00 18940644.00 20025382.00 13914791.00 17128784.00 D 15394496.00 17786998.00 36456248.00 25744574.00 12554013.00 24964122.00 21836450.00 17412368.00 22772834.00 16690849.00 28330510.00 17723504.00 E 27278836.00 27021654.00 23101306.00 28791318.00 16095923.00 21378146.00 13539052.00 22711884.00 19649032.00 18956082.00 32053366.00 21907562.00 F 13981722.00 20144692.00 18240374.00 27749628.00 18414654.00 24031444.00 13019829.00 15116972.00 19610600.00 17823786.00 18560492.00 22587032.00 G 18881148.00 22218478.00 19377964.00 27035814.00 17361300.00 14900687.00 14104549.00 19554288.00 15793969.00 10125433.00 20748736.00 28847402.00 H 17477606.00 20816876.00 28754656.00 29235188.00 21774440.00 26367080.00 14550700.00 27971524.00 22352100.00 22965644.00 22117358.00 21591098.00