Load time: 05/03/2010 12:04:01 PM Read time: 05/03/2010 12:04:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #808 Comment: Plate 51 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30569774 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12125674.00 6655017.00 13678033.00 14993158.00 22062490.00 17497428.00 20327338.00 20082828.00 15915253.00 13612855.00 20208430.00 13945284.00 B 18682738.00 13312559.00 19947020.00 26344422.00 13948604.00 21015814.00 15801749.00 22027184.00 21539710.00 14639255.00 13715551.00 18776536.00 C 16065242.00 20251562.00 18911824.00 17374696.00 16847408.00 20477212.00 10922502.00 18884958.00 18305504.00 15268530.00 13861194.00 18627970.00 D 15202146.00 16273106.00 23147072.00 23538026.00 14452085.00 19660586.00 20941356.00 24914242.00 14802032.00 14786102.00 14912623.00 9757230.00 E 13220676.00 14431096.00 24960300.00 18753304.00 15980908.00 17935186.00 17238096.00 30569774.00 18554674.00 21065536.00 16795324.00 23471082.00 F 13513207.00 12764868.00 15326566.00 28244896.00 25534548.00 24903412.00 14821859.00 21430032.00 15442594.00 11757449.00 12285036.00 18565464.00 G 18446198.00 15785452.00 20819518.00 21700028.00 26677042.00 16680676.00 25391732.00 26410694.00 13548718.00 20040208.00 14947272.00 10102825.00 H 21025000.00 18306322.00 13348166.00 20042116.00 28679648.00 19908080.00 24652176.00 16598859.00 15681514.00 19319960.00 12811640.00 13000771.00 Load time: 05/03/2010 12:06:06 PM Read time: 05/03/2010 12:06:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #809 Comment: Plate 52 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22811566 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18524186.00 19455928.00 15942287.00 9715879.00 9053952.00 11199926.00 12925233.00 13487776.00 16722570.00 17057304.00 15864530.00 12531322.00 B 12877631.00 14818786.00 13281306.00 11258308.00 9378762.00 9712502.00 14090672.00 22811566.00 10792773.00 16557302.00 13691904.00 11127838.00 C 16107828.00 13841958.00 15485661.00 16221938.00 13386148.00 9241942.00 12415074.00 13216751.00 13800224.00 9271441.00 12390351.00 14113749.00 D 14332436.00 13241079.00 21246514.00 19614350.00 14612182.00 13682141.00 16717760.00 15334773.00 16303687.00 15331634.00 8847929.00 12133966.00 E 16737228.00 19270496.00 14325637.00 16097510.00 13922007.00 20446524.00 10709492.00 14958265.00 13911342.00 10480228.00 12717108.00 13101237.00 F 11448509.00 14504177.00 18883858.00 9494425.00 16000110.00 10110825.00 12654577.00 14533210.00 11754824.00 11281000.00 14112165.00 13790233.00 G 20658690.00 13056531.00 11908621.00 13717846.00 12489635.00 11496083.00 15187536.00 18977288.00 19024654.00 11973855.00 10068017.00 9067156.00 H 13397888.00 19814654.00 17812570.00 17055960.00 8778229.00 10211576.00 11038358.00 14499938.00 21145876.00 16577137.00 15155773.00 12553273.00 Load time: 05/03/2010 12:09:40 PM Read time: 05/03/2010 12:09:40 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #810 Comment: Plate 53 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32955606 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10786452.00 14067644.00 12459352.00 11035215.00 18307998.00 15661934.00 16289710.00 14174456.00 19451412.00 20380678.00 15148670.00 19649328.00 B 14854501.00 10069123.00 10973967.00 11853265.00 17260058.00 19264920.00 9485917.00 13364033.00 11367885.00 9206575.00 15025835.00 17880396.00 C 18328284.00 16563778.00 15760414.00 15875394.00 11262507.00 21532090.00 9796001.00 14053406.00 18286768.00 32955606.00 27560806.00 21577114.00 D 22314004.00 18091126.00 12960629.00 20018990.00 18301804.00 12313289.00 20878270.00 14268776.00 11774118.00 18537764.00 9938788.00 18543364.00 E 15012719.00 15565241.00 10316160.00 17614534.00 13888030.00 10688202.00 23435916.00 13363437.00 15405337.00 16933872.00 16802570.00 11333074.00 F 12615478.00 7822546.00 7083091.00 14449162.00 12550178.00 13486771.00 15708993.00 15517804.00 10163933.00 17466630.00 11815476.00 12202541.00 G 13777551.00 11661093.00 7167609.00 11145525.00 13998961.00 15207103.00 12837312.00 16990230.00 17673920.00 16174582.00 15500544.00 6944733.00 H 14908115.00 12516665.00 9618866.00 15003351.00 11661758.00 12863257.00 14417769.00 10787647.00 10801257.00 19580678.00 11606282.00 18806226.00 Load time: 05/03/2010 12:11:49 PM Read time: 05/03/2010 12:11:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #811 Comment: Plate 55 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 47014544 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14847311.00 19433022.00 22619948.00 45597632.00 9419952.00 13987624.00 29769114.00 20244982.00 18535380.00 19522240.00 22027058.00 25192526.00 B 28253092.00 26257494.00 38693076.00 39497804.00 14141264.00 22846488.00 20894512.00 28281092.00 15603838.00 19003166.00 17715756.00 23371584.00 C 17516232.00 31333322.00 41912908.00 26684260.00 19213272.00 13150975.00 28341594.00 29686756.00 27982132.00 20657816.00 24092648.00 20532966.00 D 38394632.00 20260936.00 24988484.00 11607042.00 10492805.00 19424542.00 21616750.00 23920502.00 27111156.00 23104030.00 18931860.00 19953360.00 E 19201432.00 33136996.00 43592884.00 12363001.00 15431826.00 23823142.00 25787444.00 22545452.00 30289860.00 31516874.00 16861668.00 21664844.00 F 17333138.00 38383660.00 37428756.00 11933942.00 22529280.00 10979737.00 11021429.00 28383204.00 22110896.00 24968382.00 22109768.00 22000124.00 G 15836087.00 47014544.00 27975402.00 32311662.00 20295562.00 17163658.00 19186442.00 18222860.00 25388998.00 13662969.00 8754150.00 20370922.00 H 22959406.00 27190454.00 17811714.00 22979586.00 10013896.00 29437498.00 32300946.00 25753602.00 32665416.00 26752714.00 13253356.00 16308670.00 Load time: 05/03/2010 12:15:47 PM Read time: 05/03/2010 12:15:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #812 Comment: standard (2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 111748312 cps Min counts: 72843 counts Well list: r0(a6:h8) = cps(f2(a6:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 111748312.00 109758232.00 151298.00 B 64019240.00 62861576.00 145687.00 C 38676364.00 36590716.00 146734.00 D 22109578.00 21844174.00 146239.00 E 13356358.00 13284772.00 145745.00 F 7871385.00 7641814.00 165487.00 G 4822010.00 4609686.00 149701.00 H 2970561.00 2865113.00 151241.00 Load time: 05/03/2010 12:17:27 PM Read time: 05/03/2010 12:17:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #813 Comment: Plate 59 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 43210600 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25430522.00 26885760.00 20492674.00 20309848.00 29330544.00 22319694.00 25894504.00 15683313.00 26194280.00 28557796.00 12489214.00 21532030.00 B 21600870.00 28293728.00 26098976.00 14125752.00 14434785.00 22378554.00 35775572.00 35009796.00 29893056.00 14093501.00 19327532.00 22591806.00 C 22344854.00 18392154.00 31404814.00 23081578.00 27153304.00 31494704.00 25101934.00 3492663.00 35251544.00 17831936.00 22764172.00 21028294.00 D 28611284.00 9483826.00 16128738.00 23549980.00 36072264.00 30917976.00 20553334.00 43210600.00 17229100.00 23991074.00 20347458.00 20251086.00 E 15241883.00 9102923.00 28276472.00 19008868.00 30950648.00 34871260.00 7988348.00 36163860.00 13468677.00 16495109.00 14734616.00 38740800.00 F 19188570.00 12096848.00 28062772.00 13896651.00 36767380.00 29758198.00 28620118.00 26387792.00 20747824.00 16188329.00 16988194.00 38334928.00 G 15483076.00 18240612.00 5111534.00 25234550.00 32928442.00 24014370.00 22949328.00 30877656.00 16727705.00 17758728.00 23983762.00 8987478.00 H 19560176.00 13543325.00 18039848.00 28386100.00 29474250.00 32251198.00 18310408.00 29188778.00 19257348.00 15007108.00 18358306.00 25246572.00 Load time: 05/03/2010 12:19:22 PM Read time: 05/03/2010 12:19:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #814 Comment: Plate 60 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 44061592 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19685076.00 22458744.00 41459428.00 21381098.00 32987702.00 25736158.00 25316310.00 37119336.00 32316356.00 37797224.00 17701026.00 15006877.00 B 19451606.00 32628066.00 22692688.00 29112858.00 29208366.00 17698292.00 33032740.00 38146840.00 27814684.00 37300268.00 27249898.00 15000577.00 C 25668152.00 30576494.00 29010624.00 34033532.00 22399916.00 27489616.00 27244752.00 37853420.00 29354304.00 32101692.00 14540888.00 15189875.00 D 20516094.00 33488706.00 23330846.00 26472620.00 19139448.00 22597114.00 31456554.00 21258224.00 27046212.00 39353740.00 14157146.00 14677457.00 E 21281920.00 41725456.00 33165636.00 37175664.00 12845524.00 26834452.00 30535744.00 31542780.00 35973148.00 44061592.00 17497466.00 21821108.00 F 20483350.00 35257128.00 17053682.00 37291696.00 29657712.00 9478057.00 26763650.00 39230872.00 30657354.00 31246356.00 15201968.00 21578192.00 G 25385276.00 33455050.00 18847448.00 39566008.00 34639144.00 29516014.00 30200030.00 34095796.00 30410466.00 21503856.00 17122650.00 15250805.00 H 26936704.00 40492280.00 34642076.00 28443726.00 32736108.00 28453388.00 29807956.00 41513560.00 34831972.00 28153640.00 25582260.00 22261334.00 Load time: 05/03/2010 12:21:37 PM Read time: 05/03/2010 12:21:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #815 Comment: Plate 63 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45405484 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21617052.00 35599824.00 22133386.00 5187944.00 13813169.00 5693035.00 6548961.00 11319933.00 11001895.00 6095890.00 25776952.00 13579704.00 B 2929484.00 35844212.00 6699814.00 16085878.00 8860015.00 14258696.00 8139068.00 22153590.00 2680627.00 13375713.00 21788576.00 6253063.00 C 34244324.00 42213832.00 11122562.00 5740566.00 16423056.00 15924233.00 8795424.00 9072562.00 7633472.00 3751545.00 16429950.00 11283158.00 D 23466978.00 37117920.00 31722860.00 16972760.00 5414889.00 17452506.00 4909264.00 18900766.00 9734175.00 11015507.00 8624267.00 6431734.00 E 39298160.00 45405484.00 15613720.00 12795920.00 7832708.00 10127743.00 8653409.00 10132840.00 12870035.00 14073033.00 10455262.00 7227108.00 F 37941364.00 36769288.00 7680978.00 6595401.00 8583786.00 16834012.00 9272622.00 6138796.00 13246730.00 7693343.00 11786444.00 6648377.00 G 5676996.00 14288405.00 14913565.00 20960454.00 7412325.00 5303253.00 12475913.00 5525379.00 15918017.00 15619732.00 12907116.00 16060308.00 H 26270864.00 15021692.00 12819883.00 20702720.00 21063714.00 9619305.00 11028263.00 11558804.00 4029865.00 12261523.00 4871415.00 1918655.00 Load time: 05/03/2010 12:23:54 PM Read time: 05/03/2010 12:23:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #816 Comment: Plate 71 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 34908292 cps Min counts: 74631 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22606228.00 19535112.00 18353418.00 13339183.00 25339616.00 25417990.00 20573852.00 31114692.00 150024.00 164517.00 150176.00 154341.00 B 20989428.00 18953696.00 14854784.00 18088688.00 24534168.00 22707970.00 24852800.00 34908292.00 152230.00 159039.00 156376.00 152572.00 C 19530362.00 24958382.00 19370446.00 13665531.00 10274201.00 34228980.00 28254012.00 23153886.00 159534.00 155768.00 162158.00 149263.00 D 22065742.00 13517146.00 28206956.00 16177336.00 25976272.00 21675934.00 20339968.00 21309626.00 162976.00 154627.00 157517.00 159248.00 E 17844478.00 19841150.00 29024998.00 27522050.00 11973119.00 21707888.00 23945576.00 21628394.00 162919.00 156509.00 165981.00 156167.00 F 17388694.00 18724500.00 12502423.00 21946326.00 26162172.00 34483672.00 29468566.00 159971.00 155577.00 153809.00 163052.00 159381.00 G 24214940.00 18498852.00 20537024.00 24288930.00 22966986.00 18211446.00 28040650.00 160484.00 163585.00 157365.00 152477.00 159115.00 H 14534405.00 18296216.00 21222896.00 25005246.00 32193890.00 21042892.00 18841018.00 160561.00 166685.00 154075.00 156852.00 159229.00