Load time: 5/4/2010 9:16 Read time: 5/4/2010 9:16 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #830 Comment: Plate 70 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 108754280 cps Min counts: 66008 counts Well list: r0(a5:h7) = cps(f2(a5:h7)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 108754280 133499 237309 B 61815172 370505 141431 C 35091884 132796 224129 D 21654484 132016 143238 E 12734930 150351 283794 F 7665276 1784241 141336 G 4455918 245564 142249 H 2707515 135420 209426 Load time: 5/4/2010 9:19 Read time: 5/4/2010 9:19 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #832 Comment: Plate 61 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 51082928 cps Min counts: 58143 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21979690 25015586 22118290 42192252 38090532 40037120 22388952 37046476 23199764 19686464 19152392 24077242 B 116286 25132684 31857140 43839532 28297724 17848276 26232154 42599228 9161904 22366454 24216072 10821046 C 20828500 20536780 28369130 25110648 37534468 32379078 29861032 20965324 21733140 19984004 24492586 35679792 D 22938004 23133078 30313480 51082928 32524942 34136988 16595315 26949396 21295596 20124158 25924706 23861896 E 18435050 24779824 30555072 42918160 33390352 21200900 29235734 32126316 18846642 23609306 25410168 14918679 F 18533374 30025836 20873984 48097552 33553002 35792572 42330264 34804208 5737753 18383932 6137498 27437246 G 20880024 29409892 38309308 35457312 41304612 30754942 27006570 21989618 27975296 24261548 28403056 24453686 H 28863018 10985632 20299612 34562096 36068484 28549300 21701356 17305194 24263664 21072958 18082338 17365408 Load time: 5/4/2010 9:21 Read time: 5/4/2010 9:21 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #833 Comment: Plate 62 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 62180268 cps Min counts: 779050 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34580504 35111816 12642715 19542018 16708283 11891920 40484032 41782896 48628648 43942400 28999792 41111704 B 32757710 37903912 13138308 23780102 20340636 20307602 40538380 35840820 34861364 31705964 19960042 36735012 C 30517606 38842012 17389904 18620106 36032784 22582348 44074904 25482148 38905640 29435560 61837740 12860562 D 29218738 30049234 17778380 17612664 27188190 18719298 47229488 42897600 33563988 25358642 22296486 29556058 E 32010156 39360488 22599776 22580452 34017588 20830726 28331886 36713372 18743482 40078528 24020746 28705302 F 12872654 20508558 16807520 10055468 28222680 39616896 36167444 39676212 19254638 9821935 30308054 25051222 G 38994636 40731684 15930523 2558182 15876598 37520732 21936926 52258960 26030994 17943566 62180268 48849412 H 44867392 20420880 23146000 26411264 5847721 38784192 34509892 36168624 47074236 27513896 36028948 40948556 Load time: 5/4/2010 9:23 Read time: 5/4/2010 9:23 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #834 Comment: Plate 64 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 52823064 cps Min counts: 172680 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6575863 9150991 36402864 25775800 25766850 18591490 19834220 20764046 9969409 9806210 30142594 14366229 B 3531841 5666377 17014106 5468444 43797388 24579522 7128169 24827840 6105770 16731428 18760364 24538338 C 8292234 13136093 8067009 345361 12034771 19224610 15598697 30676710 21504808 15790483 11981501 20896770 D 7530477 18508848 20442956 6249171 23200386 15482139 29576930 10776262 24036014 19641296 20310248 17118412 E 2976392 36585960 26974124 17221760 20905742 19456464 26752460 16562995 23590076 30448190 11446086 10407988 F 7907704 26429094 5591100 48270396 21839880 16879846 22026924 19843614 26752736 21802962 17443758 18015974 G 3308033 23499490 6538488 11933379 13258356 17241158 52823064 6628839 15599415 34474612 19167850 27630114 H 6275407 28271432 12526114 15010779 36685704 32054540 30037994 20264236 7763015 30959540 22905704 15982357 Load time: 5/4/2010 9:31 Read time: 5/4/2010 9:31 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #835 Comment: Plate 66 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 39535672 cps Min counts: 765318 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16332258 12661455 17262706 16983504 24160122 19119444 29970278 1530636 22548362 22965460 13491989 13929773 B 19600872 27708932 21548350 10516977 8547683 18009728 19273408 24065342 26592292 31803816 32943604 12990171 C 5423381 22706220 18833838 20713640 31850364 23372938 20702304 26935234 27205376 25375988 4394019 16242093 D 20015272 14083254 27580130 15806311 19917226 21231638 13573181 22919246 25426094 24973968 17388082 21536496 E 10906625 26950700 15932739 21827706 15755516 1778132 34620456 28869224 33689688 23575296 18007646 17835606 F 27919652 13331184 14359450 24515942 29800264 2452453 14933836 22644972 15906714 18236540 21862192 20369088 G 17297778 17684712 20073446 13376829 36111120 2527913 25333900 23024986 31026986 28111572 17262326 28223398 H 9069620 21892232 27905650 28415490 18932748 18581336 22851884 32824370 24263058 29843300 11722800 39535672 Load time: 5/4/2010 9:33 Read time: 5/4/2010 9:33 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #836 Comment: Plate 67 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37985596 cps Min counts: 779050 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16124900 20541654 26052062 19426284 19973858 24397628 32119432 26118180 14885555 26002670 13847717 15455571 B 17834384 31703510 11412585 35386288 19346588 27520018 13734367 16953274 28294844 21080314 11839671 16153620 C 22515386 19185692 21654002 26944176 32634210 10243857 36765348 21840738 20395912 19846698 13205857 17569486 D 27373040 16765274 18318960 30887120 21595000 16061703 10553521 22819954 21124842 11055269 12763012 16668885 E 29939180 21474638 18924194 30015194 35516316 23647932 23735036 14062992 23370552 13041862 11001642 18945226 F 19771648 19474604 23171612 20374842 5957086 12564600 28795278 19111236 22569002 20527418 10159985 19824324 G 26856028 20699936 22822294 32364958 16110695 23584292 32600206 25717346 32823130 18063680 15495966 16630741 H 29995662 28740248 37985596 18882180 10218555 29040978 25421694 21595600 17230444 14801529 22726488 8122909 Load time: 5/4/2010 9:35 Read time: 5/4/2010 9:35 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #837 Comment: Plate 69 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31033290 cps Min counts: 779050 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16695213 27026994 21038244 15035286 23068004 22801920 15078532 22375318 28987492 16105801 21927108 30558186 B 13110824 19627688 14130332 19304736 15730448 18132548 19022848 24862942 14845756 19396106 21943042 18989694 C 19897238 28973906 14236694 11349852 21683716 19998728 13897047 21319256 29541040 9101884 18028608 25781004 D 17625216 18791136 15662138 5828140 11146441 19690344 16873228 17392078 26623644 20247908 18525046 23535128 E 15832910 22755300 16053957 23027846 16003697 18003194 19346878 23020698 15933945 20762940 12258009 18942140 F 19469156 14771276 17195606 19502588 21764042 17691898 17207344 14573266 18664808 16249547 25640996 19857828 G 13727712 21296702 9578469 15377199 20329542 17316118 19089882 24360552 14424400 21390260 24733330 22973248 H 15814847 14681419 21691386 15449105 24037198 17267948 18881720 18030362 25702410 22339774 20409912 31033290