Load time: 5/18/2010 12:03 Read time: 5/18/2010 12:03 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #849 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 98270472 cps Min counts: 970 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 90266768 93644464 98270472 140597 32437160 35970216 69743192 64555044 69416000 71167672 69122512 57045864 B 55792056 57313360 56528956 139207 34461064 34683456 64511256 64011884 57259076 67352936 70441224 56177912 C 33732700 34427892 34362812 138046 35178512 32206334 69281560 58841708 53384648 65743692 65395792 65703800 D 18933432 19403892 20392460 138046 31293238 33265602 61216920 62746872 64315452 63849928 69386984 69330840 E 11511047 11755444 11242887 2056 33932324 1941 34576728 34879080 34906096 37866588 36548304 35631152 F 6977395 7202669 7172096 2132 31941572 2037 35685452 35929612 37469576 37223828 35727468 37873208 G 4499016 4453131 4336348 1999 33573816 2208 35735492 36553784 34617412 37322880 35883488 36737360 H 2756378 2811244 2702952 4645 33177710 2113 36350336 37195592 37031496 36280492 36256872 35906288 Load time: 5/18/2010 12:05 Read time: 5/18/2010 12:05 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #850 Comment: atc4x p1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23126180 cps Min counts: 159426 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3116481 1025284 4024285 6000835 10627443 9709811 8095332 5730455 8098705 3951003 9959610 2748818 B 2832294 3479976 2886666 5424217 318852 6632669 10739334 23126180 5807405 5895839 4488102 8204731 C 1288964 2505172 813522 7276857 13620821 20938370 9355672 7154830 22813874 4320037 6200192 10872477 D 1459527 1687340 577943 3952311 9368890 10135334 9916850 6905915 12009184 7297642 4876479 5135804 E 5138972 2859074 6957592 7167989 9466245 1395920 5804802 6188830 11309380 5928399 3767317 6734189 F 5358922 4926719 4678636 7663658 7893655 8580588 7102552 6968959 8282315 4770182 5066578 6224447 G 3406859 4192088 2775864 611488 8854786 7474728 6543684 6957605 6826484 3328176 2417799 8681329 H 3037070 1148519 1903729 8792139 6990919 8727920 10033047 5150794 7115112 5111099 2842085 6035137 Load time: 5/18/2010 12:08 Read time: 5/18/2010 12:08 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #851 Comment: atc4x p2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26987470 cps Min counts: 307695 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26987470 6581066 3492906 4809550 7613222 5835523 8160196 10230058 10215531 9647036 7586661 8593230 B 5373544 6647551 7403937 6012092 3648522 9731550 6558036 13055161 10756993 10752706 8455312 6053028 C 7395216 7186413 8057424 10376821 6618719 5007902 7195106 10811697 11464526 7460070 8965591 5078393 D 4985405 9499213 7362115 7496833 5045077 7337420 6400920 16000378 9299896 9374241 6480687 16628864 E 4378613 2613769 8449559 3629345 4235525 7366344 6194951 18194210 8411662 7959270 7514119 6552448 F 2806477 7211074 3603884 615391 11521951 4521862 8966395 13377506 11583702 5334641 17785100 7094688 G 4321042 6500828 1840978 1055345 5569988 6484470 14538766 19065452 8138922 3066125 10256498 7172195 H 3123549 6675086 5955033 1674551 4723692 7102552 12941007 12312610 10164439 7341710 7588181 3388019 Load time: 5/18/2010 12:10 Read time: 5/18/2010 12:10 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #852 Comment: atc4x p3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 12234260 cps Min counts: 75077 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2315516 1149280 2009458 170144 150573 3763493 1967698 5906267 7997177 5014759 4389139 4121191 B 1945258 1311790 151220 2740635 9086335 4981201 5328645 5623888 7766941 11509927 12234260 5123296 C 902963 539238 1570853 2425663 1838592 3233995 4973383 5684057 4496780 2750360 8404029 7425978 D 1597100 2154195 420554 1266061 589651 2910742 5078258 4959421 885486 12177517 3435659 6701709 E 1244167 1853954 1480888 1587890 807525 3793304 3671758 8518360 3384558 9611732 2140074 6967060 F 1305774 2359946 150154 1090357 673991 3471129 5967862 4511638 6313453 1445077 2236637 7087498 G 1390171 2401792 2407198 1835871 5986875 5986104 6256372 7018446 9604865 1941960 5652379 6897492 H 1297283 1042342 2223741 7563651 8241332 5977812 163233 9009511 8174303 335758 2798605 7427859 Load time: 5/18/2010 12:12 Read time: 5/18/2010 12:12 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #853 Comment: atc4x p4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19474302 cps Min counts: 384724 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1791999 3593628 3514580 3006785 2056260 1912969 2149325 982867 1841779 1371989 769448 1513043 B 2070979 3220218 4196657 3210700 2048745 2425897 2208868 1020639 1817897 1195010 1311828 1893346 C 2193717 4125339 19474302 2582008 2065799 1478337 1778130 958860 10872704 1470874 3595662 3182715 D 2451831 4419726 10559904 3198993 2213550 1058334 1868778 2271176 1705244 1286070 3370796 2214586 E 3711864 4333806 8144609 3443081 1936478 1719789 808972 2343646 1594171 1111984 1784321 1181569 F 3308359 4081707 2974437 3984049 2144639 2738982 972263 2949518 1816044 1863009 6161329 2559269 G 2609598 4119320 2513584 2044896 2003031 1931022 907076 1659578 2338886 1475234 1225471 1461735 H 3415642 3916588 15662645 2817788 2018552 1922993 1172869 2199176 1657935 837129 1467371 1778534 Load time: 5/18/2010 12:14 Read time: 5/18/2010 12:14 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #854 Comment: atc4x p5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23466066 cps Min counts: 568633 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1439251 1929904 2484801 2502155 2285581 1925404 1886250 1868279 2338248 3804055 2331156 3790625 B 1381984 1449913 3172494 2976385 1911747 2513205 2539689 1955354 2679677 3533125 4239585 4154673 C 1523572 2824322 1600396 2226814 2575365 14394490 14006513 2104945 2353850 3694824 4127759 4122727 D 1218370 1762018 1367934 2249681 1868439 2322374 2256498 2057032 2252367 5010860 3640290 4071033 E 1409209 1810416 1936982 8867837 2244188 2091047 2063727 1902686 1696481 1137267 3643571 3309506 F 1592689 2757505 1935997 2102120 1567422 1760076 1799964 2730888 3689206 2982964 2048148 4558560 G 3042016 2200526 2696951 2191546 1911822 1938177 1968358 2541839 4896037 2860285 4035912 5043325 H 2387903 2102056 23466066 2458419 2491433 2327917 2420985 2715276 5106856 3584195 3901802 4849395 Load time: 5/18/2010 12:17 Read time: 5/18/2010 12:17 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #855 Comment: atc4x p6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18317164 cps Min counts: 305906 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3061432 3818595 12047386 5919825 7634943 6563738 11250804 5466171 8142576 8620255 7378555 685052 B 3808252 3701242 7310188 11202800 5045587 5138752 4261111 4891783 6295481 13622225 11245888 812798 C 4471116 3032453 10653766 10704793 5056944 2664960 2322096 4186244 2138201 5887200 13313237 867438 D 3028164 3620484 5072863 6429180 12954312 4663537 3371740 3894881 2085282 9214696 10163525 680083 E 5960677 3966014 7852322 7242249 5979425 3668181 18317164 3037154 2160075 8153584 8022534 1022257 F 4657494 3688164 4644312 14146005 5967994 5373844 5484316 7654240 2565687 7789130 6688908 5432405 G 3615411 4283201 7948586 8832370 4444005 5753999 3486135 3423584 4157371 8198019 6897821 1530578 H 3752910 10676403 6128369 7747626 18136532 7816769 4939364 6533377 4021417 8043993 611812 5709708 Load time: 5/18/2010 12:19 Read time: 5/18/2010 12:19 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #856 Comment: atc4x p7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16256956 cps Min counts: 265259 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3311578 14417269 2912690 5582397 4793694 5138552 2221993 3275421 5060406 7750729 4350501 10270100 B 2779863 3495824 11986091 5292041 5768872 6202066 2695475 5736573 4767549 7306558 855558 6794773 C 4163946 6172395 11458681 6298289 6950950 6552801 3681626 5048929 4569619 4391526 11116163 16256956 D 793703 4214334 7003546 6044161 6411288 7686289 5280931 2553931 8228583 10496989 3789769 3001034 E 4740220 2565707 6705738 8572749 7197257 5518512 5573428 4785953 9243861 4526813 3368758 4027322 F 3322820 4057649 6147345 7193957 8032938 8234118 4241102 3038432 10580578 5883935 2839895 5150294 G 5389812 7895238 8127750 11330430 7872164 6199275 5721622 6858116 7812924 5878421 2986269 5656807 H 530519 5234842 6292545 10419608 12723771 12689896 4718239 4832084 9208471 5271414 1614550 5187933 Load time: 5/18/2010 12:21 Read time: 5/18/2010 12:21 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #857 Comment: atc4x p8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28854222 cps Min counts: 779805 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16027246 14306286 16593266 7026231 25214394 28243796 16667747 13006818 10719744 13814582 11477806 7373156 B 18408748 17426834 16766120 8798567 24036100 26106386 12717670 14055490 14254792 13839910 8929045 13625034 C 17523010 18664030 16379752 16710895 26462434 22904134 23925332 16468548 13853235 14406136 7634703 16143404 D 17687198 13410979 12775593 21391788 23795818 13844333 19946326 17103626 8964498 8523369 6648395 16181661 E 15848194 8911870 15317546 25996466 19100522 22688820 14503774 14676455 12415250 10600615 5049707 16242024 F 12748108 19980106 19338354 22117312 23366082 20949898 11878137 12267266 24568312 8750524 17227828 6112545 G 28854222 14427833 26200414 23829994 20062146 18769330 20717252 10814111 13251362 9075358 4085697 9118859 H 17419086 17969776 27099164 24135562 23653050 26251662 6849226 7311580 14763454 11163012 3690582 9133641 Load time: 5/18/2010 12:23 Read time: 5/18/2010 12:23 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #858 Comment: atc4x p9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31106246 cps Min counts: 73963 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7597905 1107320 1707752 987055 5055108 5415783 2592954 648632 31106246 463142 1436396 1393712 B 5918248 570975 871379 1048416 4223347 1605045 2794555 416803 603092 1222825 2146716 315787 C 8844602 1008112 3115201 2949857 6865024 1641529 1489722 756788 1623448 855653 1619388 321860 D 2833807 1725670 1870687 9303846 6567801 1613330 380859 679645 1236228 619085 1240131 428550 E 1507903 1734832 26987654 1309487 4338687 197159 338537 2104283 632126 514279 653087 456593 F 872141 5465715 1078534 2160370 8730509 3289315 463104 769943 377413 400145 628128 2317033 G 1359215 2175266 4144401 887409 4136797 930759 492709 7577887 704414 1004018 425180 1863207 H 1033375 4600753 2242727 1215781 13274597 422896 520238 3755672 324411 727717 3012406 147927