Load time: 5/18/2010 12:33 Read time: 5/18/2010 12:33 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #859 Comment: atc4x standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 103669576 cps Min counts: 1155 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 97422416 99760944 103669576 153800 35349032 36734592 71423240 69533800 69821256 71947608 70925472 60245744 B 60392412 60687908 59018436 151313 35730908 35913580 69448632 70111104 61216772 71320560 71532656 59318328 C 36680280 37485732 37260508 151038 37540688 33305274 70710664 63810120 59238644 69146160 68301320 70515936 D 20262352 20122130 21985206 147401 35657076 34801960 64384608 66001320 71020656 68750896 73656664 69327328 E 12489543 12727647 12163620 2432 37400956 2310 36381044 37065648 36924268 38502108 37480420 36200192 F 7559043 7730266 7705653 2463 35325436 2348 36731196 37626100 38446164 38509576 36436144 38862128 G 4852985 4848212 4638106 2403 35523044 2383 38211124 37882880 36065328 39543584 36874528 37121116 H 3019000 2997420 2903940 5036 35196996 2606 37940820 39423784 38621996 37913644 37769324 36982752 Load time: 5/18/2010 12:35 Read time: 5/18/2010 12:35 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #860 Comment: atc4x p1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 24112936 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3215579 1039033 4163639 6151136 10907713 9872741 8658448 5857163 8299592 4039807 10195850 2800121 B 2922489 3582088 3013053 5584934 326141 6877652 11085789 24112936 5922097 6014623 4536947 8157245 C 1276614 2571147 817374 7388978 13752085 21242718 9729465 7404209 23319562 4416269 6366783 11020684 D 1531624 1671294 580036 3995801 9686959 10366145 10523915 7081917 12103529 7309574 4923632 5221564 E 5251687 2890121 7198218 7261497 9742241 1411851 6086380 6359438 11757531 6368162 3848287 6898630 F 5480401 5028843 4924701 7725000 8235613 9047058 7352499 7131671 9018278 4834718 5154230 6426784 G 3692375 4287786 2604041 620970 9327277 7794099 6865338 7197761 6994307 3381532 2482270 9352772 H 2983013 1163242 1950090 8809503 7157393 8936650 10291155 5276157 7371481 5247938 2859347 6260927 Load time: 5/18/2010 12:37 Read time: 5/18/2010 12:37 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #861 Comment: atc4x p2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 26287310 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26287310 6589229 3483555 4828131 7684319 5923157 8326231 10803352 10422903 9809056 7753522 8817821 B 5442078 6716229 7486046 6064340 3676198 9912189 6637772 13252715 10965422 10841715 8585776 6167337 C 7613783 7363889 8117621 10496362 6677191 4985948 7254463 10736138 12070218 7618167 9108095 5003737 D 5044021 9522413 7519944 7555087 5116952 7431103 6475014 16316504 9933773 9452000 6566119 16877464 E 4420450 2698313 8589585 3642460 4389576 7420474 6419871 18910754 8500180 7936391 7648778 6962345 F 2819004 7295466 3659133 607519 11986017 4588851 8896087 13168075 11811062 5457005 17804944 7299090 G 4370796 6611511 1870351 1064048 5713530 6638660 14759545 18809932 8817331 3137367 10581573 7292786 H 3130500 6699394 6033687 1672276 4863450 7271675 13505476 13377789 10392746 7423736 7587149 3425514 Load time: 5/18/2010 12:38 Read time: 5/18/2010 12:38 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #862 Comment: atc4x p3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 12517511 cps Min counts: 75174 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2349028 1193848 2153203 167807 152290 3842792 2010561 5984784 7892753 5050729 4432941 4143449 B 1991095 1315587 150348 2774498 9237405 5113458 5422931 5651697 7760343 11523690 12517511 5145844 C 902522 549702 1578123 2488753 1844943 3289631 4982224 5679152 4521493 2797505 8317158 7457983 D 1604426 2167526 431871 1274853 585732 2932064 5153628 4980505 920842 12331416 3489574 6724707 E 1276576 1876151 1495266 1616225 805906 3812890 3697087 8320383 3543424 9657614 2121881 7046898 F 1331181 2416586 151333 1084335 664743 3551700 6014213 4523245 6371434 1445658 2244967 7150108 G 1384959 2420727 2434231 1866034 6031532 6047339 6296085 7071759 9502806 1957804 5705563 6925232 H 1382167 1044469 2252771 7603937 8213543 6001072 162441 9048295 8239803 335476 2797634 7480755 Load time: 5/18/2010 12:40 Read time: 5/18/2010 12:40 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #863 Comment: atc4x p4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 19566128 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1824978 3646962 3549984 3049397 2070131 1947716 2207987 1030994 1893452 1376706 776240 1548910 B 2084816 3219557 4203569 3292359 2095277 2435724 2267611 1043990 1855344 1208841 1315491 1930465 C 2184688 4209588 19566128 2636238 2108232 1494469 1807541 944669 11060610 1491439 3684320 3225221 D 2449781 4521442 10834759 3231418 2250804 1053766 1884682 2310097 1727262 1305133 3381748 2066390 E 3683841 4382185 8269761 3470625 1972237 1737892 844615 2373471 1622571 1113029 1796166 1201050 F 3270453 4057192 3018105 4069235 2179655 2768981 990004 2914559 1852672 1894103 6211494 2615927 G 2644882 4149383 2578982 2103177 2064284 1973060 933302 1616893 2392586 1493904 1250837 1488014 H 3438259 3964747 16147231 2853663 2050945 1966369 1292142 2230559 1697153 854896 1489771 1816978 Load time: 5/18/2010 12:42 Read time: 5/18/2010 12:42 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #864 Comment: atc4x p5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 24432068 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1448790 1956762 2513466 2556070 2309364 1949113 1905625 1898542 2358037 3873337 2359060 3888992 B 1409204 1477339 3201869 3060826 1943972 2548921 2575367 1960905 2704592 3544500 4269383 4241076 C 1532650 2881257 1609501 2270647 2617894 14319952 14204893 2143123 2399681 3713309 4132964 4131158 D 1230419 1746422 1424895 2307055 1920661 2324179 2276449 2087107 2297507 5087730 3665419 4116056 E 1456579 1839130 1968329 9055933 2297467 2137554 2099655 1644198 1723257 1146134 3706530 3369733 F 1601374 2811825 1969477 2158332 1599695 1810253 1820215 2750055 3733850 2981691 2056716 4643478 G 3171804 2254653 2745812 2236346 1945115 1966549 2001770 2582019 4896812 2895815 4184777 5105696 H 2435747 2157487 24432068 2515234 2560341 2376917 2464483 2799024 5185815 3608172 3990157 4883618 Load time: 5/18/2010 12:44 Read time: 5/18/2010 12:44 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #865 Comment: atc4x p6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 19647002 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3161004 3899404 12161943 5897940 7713215 6507340 11321209 5645427 8297162 8710323 7564014 689441 B 3900899 3775244 7562357 11516502 5116358 5300077 4392224 4937137 6481570 14036601 11130400 825732 C 4571225 3081274 10921069 10622212 5137917 2743042 2343072 4272117 2192224 5989255 13310047 878459 D 3116342 3586539 5160563 6582345 13377789 4986386 3502986 3953905 2122636 9535110 10182510 682596 E 6065498 3891948 7984931 7343996 6097882 3686750 19647002 3128686 2196999 8327715 8276573 1031816 F 4764121 3718022 4723272 14668179 6104682 5456593 5594341 7893382 2638990 7976826 6718560 5577909 G 3790477 4625969 7959869 8919779 4510907 5895049 3539190 3604846 4246475 8249907 7107789 1562028 H 3767270 11011207 6301433 7831162 18940538 7966339 5177933 6676742 4149536 8125100 623042 5563562 Load time: 5/18/2010 12:45 Read time: 5/18/2010 12:45 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #866 Comment: atc4x p7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 16269017 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3415683 14336245 2982498 5809283 4917289 5186695 2317860 3318830 5088285 8021887 4450263 10564955 B 2838530 3568994 12138881 5424043 5868855 6324478 2728849 5824671 4899987 7409254 869786 6880870 C 4220353 6171172 11530386 6189843 7084003 6783380 3832902 5221083 4701647 4496470 11278101 16269017 D 818270 4273773 7118050 6085400 6494084 7824368 5247071 2629627 8294388 10780757 3967402 3079373 E 4886774 2643358 6612447 8659393 7395862 5695043 5696923 4874531 9261978 4546015 3416712 4098565 F 3345109 4090666 6307742 7214180 8031790 8973726 4314330 3086017 10551473 6125220 2898299 5191478 G 5448279 8045303 8135426 11556909 7994741 6266710 5835676 6998808 8210739 5918344 3075639 5754243 H 528176 5332764 6437573 10573961 12633857 12999228 4853222 4956621 9275399 5317652 1652358 5219538 Load time: 5/18/2010 12:47 Read time: 5/18/2010 12:47 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #867 Comment: atc4x p8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 28372216 cps Min counts: 79379 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16032110 14208962 16645303 7014828 26147036 28359038 17325022 13030810 10959820 13856026 11719515 7388083 B 18852370 18135662 16601094 8881058 24862124 26200544 12799682 14590263 14874043 13929699 8951261 13829231 C 17461458 18824204 16522998 17734536 26535114 23573720 24529460 17039408 14012566 14842892 7741122 16285037 D 18095972 13556912 12792049 21697358 23845652 14209725 20395628 18043732 9391612 8799835 6716570 16084738 E 15443538 8882053 14996275 26536888 19359980 23765692 14568575 14785112 12473058 10627189 5159691 16093868 F 12867946 19936656 19491182 22293936 23753602 21593478 12066548 12786691 25764292 9094634 17260980 6101584 G 28372216 14270268 26324794 24480288 20989948 19612538 21926908 11124473 13829231 9416904 4159646 9310756 H 17265484 18521580 26954088 24137134 24183460 26535998 7050904 7647894 15501746 11391394 3743976 9123589 Load time: 5/18/2010 12:49 Read time: 5/18/2010 12:49 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #868 Comment: atc4x p9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 32.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 31190488 cps Min counts: 72043 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7682014 1071302 1643014 1017222 5056810 5430945 2623058 637835 31190488 452717 1404179 1408911 B 5884997 565765 847963 1045903 4239716 1590165 2785822 407900 607492 1233695 2164257 292877 C 8866178 1002492 3081035 3013156 6936488 1663738 1477473 757397 1638774 866787 1608014 316893 D 2834243 1705636 1923416 8551921 6884332 1612653 388536 684732 1238942 618257 1252005 425819 E 1475606 1763015 27496552 1316652 4470640 200219 365391 2101423 665328 508289 647885 458074 F 835852 5596431 1078370 2401387 8805496 3291349 481691 759153 370380 393064 622126 2353521 G 1311089 2168550 4162460 894769 4103099 931580 498818 7708496 700026 1005439 445056 1889774 H 1105874 4693613 2269745 1197654 13256034 493719 561985 3897432 318394 700317 3110383 144087