Load time: 05/20/2010 02:17:21 PM Read time: 05/20/2010 02:17:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #870 Comment: standards_watson Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 102717736 cps Min counts: 440477 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 102717736.00 97140624.00 100512312.00 1695574.00 4359213.00 4893739.00 1507791.00 4454804.00 4600363.00 1550106.00 4318842.00 4497554.00 B 73694512.00 71834496.00 74084184.00 924487.00 5339919.00 7980498.00 880955.00 5482010.00 7560509.00 1003165.00 5280518.00 7265198.00 C 44957604.00 48133344.00 42766816.00 1684664.00 2966046.00 4799485.00 1613280.00 2910057.00 4811054.00 1550696.00 2879975.00 4798160.00 D 27088658.00 28206760.00 27534470.00 1573690.00 1878068.00 5249445.00 1497711.00 2282591.00 5080799.00 1413480.00 2108862.00 4728320.00 E 15718116.00 15035591.00 15104101.00 1174613.00 2839041.00 4375186.00 1163316.00 3086992.00 3807024.00 1087035.00 2741723.00 3758564.00 F 9083504.00 8755778.00 8608403.00 1210615.00 5346938.00 6556158.00 1046431.00 5654110.00 5978003.00 1151183.00 5331608.00 5741907.00 G 5628239.00 5187131.00 5162038.00 1015318.00 1658806.00 5866329.00 1039376.00 1625154.00 5061022.00 1107441.00 1715608.00 4967845.00 H 3268983.00 3306410.00 3116113.00 2097093.00 2499035.00 3079416.00 1474167.00 2719327.00 2987353.00 1466370.00 2852044.00 2776887.00 Load time: 05/20/2010 02:19:29 PM Read time: 05/20/2010 02:19:29 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #871 Comment: helen Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28771312 cps Min counts: 65726 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23093082.00 22439884.00 21562220.00 22792466.00 22564230.00 22149002.00 22990166.00 21995032.00 22958930.00 20615910.00 19759342.00 20600810.00 B 10807434.00 9853801.00 9785388.00 25375362.00 23630674.00 19388476.00 13886633.00 13549394.00 13469154.00 19127208.00 18631618.00 19354898.00 C 15214610.00 14613345.00 14573238.00 23303860.00 23134780.00 21779610.00 21792162.00 21717132.00 23086648.00 16096700.00 15501758.00 15771612.00 D 20765668.00 19367844.00 18848092.00 23512412.00 21727610.00 20975520.00 17815854.00 17023098.00 18065450.00 16792496.00 16783272.00 16992984.00 E 28771312.00 26192758.00 26889674.00 25741292.00 23403838.00 22640962.00 18469450.00 18563064.00 19121948.00 16764633.00 16473214.00 16849198.00 F 24578632.00 23604324.00 23283382.00 21982618.00 22030674.00 20142016.00 19296498.00 18349676.00 20081164.00 138242.00 138014.00 133335.00 G 25153394.00 24151512.00 23664844.00 25664712.00 24090786.00 23884190.00 19847596.00 18853888.00 19180380.00 139022.00 136682.00 132708.00 H 24992476.00 23510146.00 23114734.00 20442246.00 19115380.00 18583394.00 23978440.00 23243226.00 23963174.00 136283.00 131452.00 132765.00