Load time: 05/26/2010 11:35:12 AM Read time: 05/26/2010 11:35:12 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #875 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 124319632 cps Min counts: 923 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 124319632.00 76216048.00 82641664.00 118757496.00 119458096.00 111400912.00 153575.00 2055.00 1960.00 2169.00 2074.00 1884.00 B 60709560.00 62906672.00 63629088.00 78850152.00 78884456.00 79754424.00 155041.00 2150.00 2074.00 2112.00 1998.00 2036.00 C 35915576.00 37062668.00 39881700.00 48027276.00 46585688.00 46383908.00 152034.00 1998.00 2093.00 2245.00 2055.00 1998.00 D 21200468.00 21552128.00 22203908.00 28556802.00 28317704.00 28751008.00 149959.00 1998.00 2093.00 2188.00 2112.00 2017.00 E 12588141.00 12431051.00 12741041.00 15920525.00 15895530.00 15751644.00 146704.00 2036.00 2188.00 2055.00 2150.00 2093.00 F 7712616.00 7693421.00 7917359.00 9435214.00 9340613.00 9152051.00 153899.00 2055.00 2150.00 1998.00 2074.00 2093.00 G 4660415.00 4663506.00 4813997.00 5783694.00 5715229.00 5633972.00 154965.00 1846.00 2188.00 1865.00 2112.00 1998.00 H 2885015.00 2880697.00 2992398.00 3292664.00 3398120.00 3640394.00 153556.00 2036.00 1979.00 2074.00 1960.00 1960.00 Load time: 05/26/2010 11:37:46 AM Read time: 05/26/2010 11:37:46 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #876 Comment: plate10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13719798 cps Min counts: 72629 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1362292.00 1839215.00 477913.00 5570010.00 3420758.00 3540817.00 1569152.00 3503751.00 2113112.00 2285219.00 3137609.00 2498633.00 B 1244764.00 1527458.00 4464383.00 12104921.00 11376729.00 3670067.00 2851533.00 2485055.00 7990181.00 4242027.00 2583023.00 3891677.00 C 1924994.00 5330770.00 3988839.00 5246079.00 13719798.00 5431037.00 4792842.00 840736.00 3379547.00 1554656.00 2419633.00 5368596.00 D 4744607.00 3329317.00 1866519.00 6140217.00 1072327.00 3712431.00 4578960.00 1148363.00 1220783.00 3183893.00 7688337.00 1221906.00 E 2804471.00 2577839.00 5750575.00 6106965.00 3843484.00 3555175.00 3077483.00 2737367.00 4185245.00 3697845.00 2352706.00 1491258.00 F 2965716.00 1929774.00 4945391.00 12163546.00 4383258.00 7367496.00 7529829.00 3782811.00 7032557.00 1903933.00 3051313.00 5904164.00 G 1693183.00 6739144.00 4092680.00 3780077.00 4586991.00 1666504.00 2236758.00 5281835.00 2305208.00 2693747.00 2059550.00 147694.00 H 2553955.00 2480190.00 6864537.00 1202949.00 3569857.00 1051143.00 1230071.00 1909113.00 2453662.00 3236306.00 3112444.00 145258.00