Load time: 06/02/2010 01:14:54 PM Read time: 06/02/2010 01:14:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #888 Comment: standards Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 283346720 cps Min counts: 561 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 283346720.00 199234496.00 248742880.00 239962.00 1313.00 1294.00 1351.00 1447.00 1313.00 1351.00 1523.00 1351.00 B 141709392.00 152966336.00 125621528.00 264962.00 1466.00 1408.00 1389.00 1485.00 1485.00 1447.00 1351.00 1408.00 C 78944368.00 74923960.00 69425416.00 327566.00 1389.00 1275.00 1256.00 1428.00 1504.00 1447.00 1389.00 1428.00 D 52509272.00 31620318.00 48551548.00 262753.00 1313.00 1408.00 1294.00 1332.00 8872.00 1332.00 1332.00 1523.00 E 38452760.00 23734100.00 23271838.00 1294.00 1370.00 1294.00 1389.00 1504.00 1332.00 1466.00 2627.00 1389.00 F 17301178.00 21408492.00 15241150.00 1180.00 1466.00 1789.00 1428.00 1199.00 1351.00 1408.00 1408.00 1389.00 G 10980084.00 9291782.00 10057096.00 1523.00 1428.00 1485.00 1389.00 1351.00 1294.00 1275.00 1370.00 1370.00 H 5716024.00 6855727.00 5696343.00 1408.00 1428.00 1123.00 1332.00 2303.00 1351.00 1466.00 1408.00 1523.00 Load time: 06/02/2010 01:17:55 PM Read time: 06/02/2010 01:17:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #889 Comment: jmm-weeeeeeeeeeeeeeeeeeee Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 157453616 cps Min counts: 143952 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16756807.00 7962545.00 6293576.00 6155229.00 8277480.00 9352590.00 9346740.00 11070264.00 12206756.00 157453616.00 5003432.00 17035388.00 B 4733489.00 13279449.00 11369211.00 8110370.00 10834770.00 39421904.00 5842791.00 3885596.00 9238924.00 9960452.00 7577518.00 12217791.00 C 5192525.00 3529660.00 10187671.00 5845252.00 44917692.00 3792434.00 32794808.00 3062875.00 22216750.00 3382526.00 28144580.00 15500914.00 D 2773092.00 4516382.00 46310820.00 6454023.00 21925748.00 4550798.00 4760927.00 11381855.00 20940462.00 37513880.00 11491419.00 12694636.00 E 38129216.00 49533708.00 54499576.00 35811928.00 13124578.00 5842699.00 14980158.00 31639814.00 5720003.00 5139736.00 14820935.00 4348336.00 F 5275008.00 23410014.00 287905.00 12440496.00 11722670.00 26772244.00 4813986.00 5225791.00 6140394.00 16717508.00 7830494.00 10636883.00 G 4052264.00 8842146.00 3725231.00 8605146.00 12314054.00 15026773.00 10095305.00 38528940.00 59938020.00 24092342.00 6491845.00 49556692.00 H 3224944.00 7969266.00 28212490.00 66105832.00 19113854.00 31456174.00 7212815.00 31062392.00 13426337.00 23672002.00 2691164.00 3168701.00