Load time: 7/14/2010 14:17 Read time: 7/14/2010 14:17 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #907 Comment: Standards Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 1980826 cps Min counts: 52334 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 104669 874791 108619 301262 B 1980826 130163 1019138 110774 C 110504 143988 247670 683315 D 202247 142192 111222 111312 E 1012585 299825 105746 117596 F 128368 104939 109786 112300 G 305481 110684 110504 109696 H 633763 105746 169123 108619 Load time: 7/14/2010 14:18 Read time: 7/14/2010 14:18 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #908 Comment: Standards sens 2 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2444324 cps Min counts: 54508 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 111835 919957 112959 288444 B 2444324 134214 1046362 115358 C 118520 145717 248487 674707 D 228146 146860 117015 115568 E 1177129 330116 111511 122043 F 140156 109016 115435 118006 G 306557 115816 116806 115282 H 689106 112444 173885 114044 Load time: 7/14/2010 14:20 Read time: 7/14/2010 14:20 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #909 Comment: Standards sens 0 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 0 A/D converter gain: x1 Integrating gain: x1 Max cps: 2758781 cps Min counts: 53266 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 113130 919593 112159 262741 B 2758781 130400 1029424 113906 C 117593 142237 241977 632790 D 221796 144759 116428 113906 E 1181170 328717 108472 117205 F 138744 106532 112547 114682 G 298251 114682 114294 114876 H 683631 109443 170762 110025 Load time: 7/14/2010 14:22 Read time: 7/14/2010 14:22 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #911 Comment: plate 1 sens 1 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 17401202 cps Min counts: 2461400 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6342665 6308733 9631810 7331103 8393655 6775527 6851202 14061922 11947346 13509487 12494943 7640061 B 6762062 8088836 8363546 8997517 9160044 7823693 11789962 14489623 12046790 14203625 11262534 9407403 C 6711164 7933796 10126899 10278661 10405328 8837423 7874543 16677160 13809807 13871962 12583244 12666254 D 5969319 7792072 11438341 11423221 10505724 11147776 8492787 16662536 16715174 17401202 15605017 9485592 E 6791506 7447886 9847780 10921831 11288641 10037083 11914204 16338752 15132567 17370676 13751173 9670942 F 7344658 6307027 8896243 8708131 9519831 9835408 12466547 11727400 13668600 14686015 14105302 8789375 G 4922801 5746784 6092930 7344389 8108660 7066736 7087472 12631616 11618435 11788446 9296690 6026322 H 5178281 6992497 6807305 7135318 7519284 7487611 6601646 7845459 6951922 9152133 8373908 7030290 Load time: 7/14/2010 14:25 Read time: 7/14/2010 14:25 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #912 Comment: stan sens 1 mow Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 1475520 cps Min counts: 65126 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 130253 531697 143898 227921 B 1475520 175676 604409 141744 C 131869 149374 202876 439684 D 304045 184294 139320 141654 E 340760 234115 133485 148925 F 161672 132497 155209 145334 G 349826 150361 145693 143000 H 419487 135998 165712 142462 Load time: 7/14/2010 14:27 Read time: 7/14/2010 14:27 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #913 Comment: plate 1 sens 1 mow Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 22316488 cps Min counts: 3171916 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7796148 8018941 12402269 9475251 10821881 8782749 8819756 17944210 15388579 17119266 15533310 9370558 B 8550467 10325860 10739822 11709029 12282982 10253007 15673010 18425014 15449662 17928636 14158465 11563549 C 8599523 10334480 13075589 13523361 14079135 11543744 10224136 21575800 17603990 17477910 15914011 15871737 D 7585223 10145248 14964849 15620384 13578540 14736446 10991545 21094322 21499374 22154606 20001840 12071399 E 8799142 10058390 12974189 14548531 14958316 13021150 15452000 21032444 19809342 22316488 17585814 12163115 F 9551877 8739532 11745040 11722374 12713210 12930200 16434338 15432135 17984364 19438120 18735832 11460283 G 6343832 7555928 8177002 9691804 10893086 9319502 9367054 16670868 15292161 15475360 12531903 7922972 H 6719692 9230834 9093933 9553378 10420615 10213120 8679731 10507893 9298747 12391866 10733758 9237206 Load time: 07/14/2010 02:30:43 PM Read time: 07/14/2010 02:30:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #914 Comment: plate 2 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 37839004 cps Min counts: 3676907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12535660.00 16124226.00 21081284.00 19742154.00 29950408.00 22947582.00 21344120.00 30139172.00 27280624.00 31144022.00 25891868.00 31823286.00 B 14786344.00 20568866.00 21065340.00 24735296.00 25322476.00 23881462.00 34526988.00 31805144.00 22987866.00 31479640.00 22936270.00 22411704.00 C 17254908.00 21451484.00 26752602.00 28309642.00 28507642.00 29146850.00 25647816.00 32354608.00 27308908.00 31682286.00 27057766.00 30394012.00 D 16395973.00 24582264.00 29741486.00 32877080.00 30751726.00 25591924.00 25348344.00 37839004.00 30480866.00 35645180.00 35510848.00 23776574.00 E 17902754.00 32403564.00 28754704.00 31192450.00 27434116.00 29654054.00 36409884.00 36210732.00 28863340.00 34571984.00 30995660.00 23816708.00 F 23688434.00 30351816.00 27746262.00 30672866.00 28236334.00 26990414.00 32869614.00 30533946.00 28247718.00 30025736.00 31079368.00 28991170.00 G 19529572.00 25217336.00 23571606.00 21777966.00 25883282.00 23745236.00 25347190.00 29460694.00 25962310.00 27569428.00 23766368.00 19572154.00 H 14436391.00 18943870.00 19473688.00 20504688.00 21213430.00 19402670.00 16965686.00 22599762.00 21576142.00 21154260.00 20317152.00 15966080.00 Load time: 07/14/2010 02:32:33 PM Read time: 07/14/2010 02:32:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #915 Comment: plate 2 sens 1 mow Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 37589652 cps Min counts: 3676907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12608841.00 16123710.00 21161532.00 19894152.00 30640636.00 23195898.00 21477358.00 30656140.00 27758222.00 31322600.00 26231580.00 32402280.00 B 15009132.00 20831378.00 21193058.00 24914196.00 25525568.00 24108890.00 34903972.00 31817944.00 23181550.00 31821248.00 23096136.00 22569436.00 C 17391992.00 21568586.00 26979244.00 28501386.00 28639958.00 29120440.00 25794418.00 32622200.00 27546768.00 31707506.00 27401814.00 30798168.00 D 16562726.00 24938174.00 30025736.00 32577226.00 30586752.00 25628134.00 25508586.00 37589652.00 29811872.00 35473264.00 35795680.00 23864180.00 E 18009098.00 33049422.00 29010546.00 31283774.00 27632264.00 30070624.00 36661064.00 36161448.00 29151420.00 34763624.00 30894952.00 23928630.00 F 23964752.00 30687202.00 28187002.00 30568722.00 28480790.00 27282688.00 33422778.00 30903778.00 28582054.00 30342098.00 31240316.00 29605108.00 G 19825566.00 25324584.00 23694250.00 22293512.00 26337158.00 24281390.00 25322614.00 29517812.00 26224300.00 27913380.00 23776280.00 19606342.00 H 14549969.00 19108458.00 19536130.00 20620766.00 21355190.00 19591250.00 16948684.00 22739108.00 21634476.00 21156976.00 20389552.00 16032328.00 Load time: 07/14/2010 02:35:21 PM Read time: 07/14/2010 02:35:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #916 Comment: plate 3 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 60541860 cps Min counts: 116788 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27454130.00 36108892.00 24804832.00 28932954.00 27901074.00 31120374.00 34038816.00 32748066.00 26224376.00 27413948.00 26163360.00 27442470.00 B 24457032.00 23436882.00 22382440.00 24876372.00 27962674.00 35068560.00 36604276.00 27971822.00 28488292.00 33069636.00 29670682.00 24242442.00 C 25179226.00 34944016.00 24077580.00 17185486.00 20871712.00 31631510.00 36063380.00 35429516.00 30869092.00 30978140.00 29959096.00 26437068.00 D 27233302.00 35525364.00 21807882.00 27604630.00 27643214.00 34418880.00 39007680.00 34683480.00 34394220.00 30491508.00 34866968.00 29363178.00 E 30937244.00 38559260.00 29351248.00 18902024.00 24197116.00 34857548.00 34845824.00 29297942.00 31342226.00 32936954.00 27595124.00 27100162.00 F 28454186.00 35045900.00 19889516.00 26955786.00 24620650.00 31851900.00 37554712.00 33405504.00 30828972.00 28114176.00 31867718.00 25584782.00 G 22397110.00 23900340.00 15125223.00 10160926.00 23207668.00 27390504.00 32550262.00 22735002.00 60541860.00 25769382.00 24248038.00 24034928.00 H 21686356.00 24703088.00 21398402.00 26121278.00 233576.00 25069080.00 24059904.00 26473402.00 23054284.00 24414550.00 24128696.00 18501026.00 Load time: 07/14/2010 02:37:47 PM Read time: 07/14/2010 02:37:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #917 Comment: plate 4 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 34012684 cps Min counts: 3462316 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29206070.00 27493996.00 27596700.00 27204992.00 28530622.00 29160394.00 22734044.00 30682310.00 30513600.00 30860722.00 29138578.00 30245978.00 B 26086900.00 21756498.00 24456576.00 19919890.00 29138738.00 32253882.00 21179032.00 29701602.00 18237050.00 19072340.00 26502846.00 31363506.00 C 27982526.00 29298364.00 26197344.00 24404844.00 18233588.00 29392376.00 31122434.00 27462556.00 21733196.00 27643382.00 25807020.00 33843156.00 D 26638752.00 24761402.00 25090052.00 26987048.00 27930088.00 34012684.00 32066316.00 28558080.00 31995334.00 29534526.00 29750700.00 30499248.00 E 23355152.00 18940660.00 25717274.00 26892484.00 28332652.00 31941466.00 29576410.00 28249804.00 28321260.00 29871888.00 20070954.00 31197160.00 F 23370992.00 6924632.00 23207874.00 24716560.00 21831408.00 32523602.00 17558864.00 26092530.00 24320918.00 22283190.00 26295550.00 29255520.00 G 21764828.00 21105652.00 21356330.00 20964750.00 20741964.00 29155076.00 28021852.00 24406952.00 22024198.00 22565020.00 25669230.00 24793456.00 H 21071112.00 23917750.00 21402912.00 20403478.00 20041178.00 25004516.00 24492792.00 27407000.00 24176212.00 22637036.00 23835700.00 28042046.00 Load time: 07/14/2010 02:40:07 PM Read time: 07/14/2010 02:40:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #918 Comment: plate 5 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 18391956 cps Min counts: 3676907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14623908.00 17704546.00 14258794.00 14991170.00 15603598.00 14358672.00 18051120.00 15553536.00 13799727.00 15029831.00 13488361.00 14296172.00 B 14202517.00 13463060.00 13389232.00 11080557.00 15050107.00 18391956.00 16525898.00 16147657.00 9791101.00 13263010.00 13338897.00 15046467.00 C 11829432.00 13652932.00 14286102.00 14832542.00 10185815.00 16951044.00 16752826.00 15867977.00 12416182.00 14189528.00 14029777.00 15105264.00 D 14361408.00 12472159.00 14363270.00 18174592.00 15377233.00 16394285.00 16393378.00 15213318.00 16305530.00 16179259.00 16701120.00 15496479.00 E 14864735.00 9899732.00 15334075.00 15523033.00 16136283.00 18003278.00 18096190.00 14200007.00 14902560.00 17841882.00 9891439.00 15516293.00 F 13223449.00 12236928.00 13898706.00 14892178.00 10349874.00 16029930.00 15751458.00 15888520.00 14907295.00 14235549.00 15898660.00 15224530.00 G 9398179.00 8965597.00 11442412.00 14708487.00 15018824.00 13797297.00 14202200.00 16056101.00 13840660.00 15088429.00 14198936.00 14910379.00 H 12845750.00 15614313.00 13407447.00 16094630.00 12670194.00 15986794.00 15365851.00 16250538.00 15943503.00 14926558.00 12677665.00 15205818.00 Load time: 07/14/2010 02:42:41 PM Read time: 07/14/2010 02:42:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #919 Comment: plate 6 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 10289820 cps Min counts: 2076429 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7454287.00 7092319.00 6997345.00 7485119.00 7759171.00 6998153.00 8196205.00 8062071.00 7227690.00 8411846.00 8487366.00 7619227.00 B 7476007.00 6535487.00 7351061.00 5620749.00 7016196.00 8005765.00 7445514.00 7344209.00 4152858.00 8762522.00 7413644.00 9990427.00 C 7810349.00 7553703.00 7474310.00 7333347.00 5259880.00 7549729.00 7635301.00 7476056.00 6475342.00 7864969.00 8589186.00 9562272.00 D 7933841.00 7887846.00 7839629.00 7605809.00 7364430.00 8026122.00 7739794.00 7701330.00 8089995.00 8625355.00 9794324.00 9981085.00 E 8233648.00 6651019.00 8912528.00 8819710.00 8601380.00 8781340.00 7364481.00 7203991.00 7405411.00 10152186.00 6260796.00 10289820.00 F 7265034.00 5118316.00 7872122.00 6695275.00 5828204.00 7991717.00 7079393.00 7833837.00 7175894.00 7226074.00 8915365.00 8678232.00 G 6927236.00 6366274.00 6978404.00 6627410.00 6655777.00 7455301.00 7940380.00 7124277.00 6071744.00 6705867.00 7977243.00 7866590.00 H 7048513.00 6748148.00 6931724.00 6788813.00 5841849.00 8011156.00 7939556.00 7688473.00 7043486.00 7307853.00 6359003.00 8520850.00 Load time: 07/14/2010 02:45:47 PM Read time: 07/14/2010 02:45:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #920 Comment: plate 7 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 30922492 cps Min counts: 1964533 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12469080.00 12485206.00 12213601.00 17233980.00 24455066.00 14195498.00 17317548.00 16965578.00 15962630.00 16770133.00 18821446.00 16786142.00 B 12703938.00 12542398.00 11595238.00 12094829.00 23157196.00 25507684.00 15983611.00 15684991.00 9759904.00 15335257.00 19032830.00 18228282.00 C 13639294.00 12479647.00 12138267.00 11092571.00 11027235.00 20932344.00 9280835.00 16821522.00 14982350.00 17399818.00 16350332.00 16850522.00 D 12282354.00 10208073.00 3929066.00 6613765.00 7786326.00 14200691.00 11515553.00 13702034.00 17839130.00 16187451.00 21523778.00 19792662.00 E 12446236.00 7424381.00 6476061.00 5913663.00 10176813.00 13422978.00 13895503.00 15140314.00 14624594.00 17939370.00 11874093.00 17402396.00 F 11727741.00 10518001.00 12023998.00 12188600.00 11618493.00 25579604.00 14652644.00 15916802.00 16497945.00 14618065.00 18092984.00 17823392.00 G 12191437.00 12232030.00 11045322.00 18772706.00 19332676.00 17983564.00 15948033.00 14791716.00 13660897.00 14958354.00 17123716.00 15800580.00 H 19675946.00 18142128.00 17671906.00 18645704.00 18933656.00 30922492.00 18245504.00 19933918.00 17980258.00 18060192.00 18168152.00 18086184.00 Load time: 07/14/2010 02:52:09 PM Read time: 07/14/2010 02:52:09 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #921 Comment: plate 8 sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 26141672 cps Min counts: 3676907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18138288.00 19987196.00 21797784.00 19516886.00 19982854.00 22103262.00 21094394.00 14000389.00 16207909.00 17236022.00 18414384.00 17378708.00 B 18628598.00 18232430.00 18352244.00 19715804.00 20675446.00 23198384.00 22046278.00 16116323.00 17129906.00 16822720.00 17314334.00 19891676.00 C 19052160.00 20291936.00 17986518.00 20728922.00 22998102.00 25325912.00 21825744.00 17571030.00 17543206.00 17752646.00 17761076.00 14841048.00 D 19187034.00 19196698.00 20880352.00 21116334.00 21120472.00 24207712.00 22245738.00 18946888.00 19645984.00 19646280.00 19897156.00 14976126.00 E 19647106.00 20099528.00 21123420.00 22374378.00 22111558.00 24772846.00 22797616.00 19491682.00 18103398.00 19860220.00 18983914.00 18436120.00 F 19413642.00 19465854.00 18350358.00 20363332.00 20724518.00 26141672.00 23242832.00 17358066.00 18242678.00 16909844.00 12965591.00 18884464.00 G 16958612.00 18189864.00 18776876.00 19364962.00 19816974.00 23489568.00 22115256.00 18058834.00 17669824.00 17841354.00 17482474.00 16302010.00 H 18008560.00 18448634.00 19325036.00 18789782.00 19182666.00 22378152.00 20597994.00 19431174.00 20895650.00 22876522.00 20664000.00 18924644.00 Load time: 07/14/2010 02:54:41 PM Read time: 07/14/2010 02:54:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #922 Comment: standard (real) sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 95909384 cps Min counts: 67101 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 93909320.00 95909384.00 91586088.00 135101.00 B 58475168.00 59354916.00 58471916.00 134203.00 C 34569316.00 35058128.00 34392284.00 143449.00 D 20436080.00 20563666.00 20303318.00 137704.00 E 11637968.00 11634713.00 11619117.00 144796.00 F 7063055.00 7054617.00 7123469.00 139679.00 G 4270275.00 4264530.00 4280239.00 137076.00 H 2887934.00 2593315.00 2629850.00 137165.00