Load time: 07/15/2010 10:20:39 AM Read time: 07/15/2010 10:20:39 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #924 Comment: standard (real) sens 1 3mm Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 56925056 cps Min counts: 5251 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 50588712.00 56780104.00 56925056.00 13375.00 B 32659768.00 35904720.00 33400844.00 22083.00 C 19522686.00 10767570.00 19378084.00 22173.00 D 10740255.00 12126488.00 11775548.00 11580.00 E 5233955.00 4938427.00 4580507.00 10503.00 F 2758053.00 3217684.00 3466082.00 13375.00 G 772844.00 2079470.00 1088121.00 31060.00 H 1357436.00 1434281.00 1494967.00 23789.00 Load time: 07/15/2010 10:22:45 AM Read time: 07/15/2010 10:22:45 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #925 Comment: Plate 1 (315) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 44435004 cps Min counts: 3677045 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35307152.00 40782696.00 40675820.00 39044604.00 37588760.00 39824688.00 32953308.00 29553138.00 34741844.00 29966012.00 33327976.00 9543418.00 B 35083468.00 35544248.00 32033278.00 39237724.00 44435004.00 37486028.00 28864882.00 38995580.00 37469104.00 29210164.00 26149200.00 16732996.00 C 36029016.00 33043958.00 34586296.00 32149312.00 42054328.00 39497524.00 28928512.00 35379340.00 42364864.00 28261900.00 33277272.00 13537681.00 D 39736676.00 35069880.00 33439478.00 28834146.00 43310416.00 40594680.00 32683340.00 37867360.00 42842432.00 30569366.00 33036950.00 25138084.00 E 38749876.00 33591980.00 32794106.00 34551100.00 38478392.00 41214116.00 30112814.00 30470524.00 38869896.00 28034384.00 28647064.00 44202696.00 F 26702472.00 33446508.00 33146066.00 31015002.00 42701476.00 38544708.00 25029224.00 28567564.00 37279128.00 28919368.00 32000884.00 13599049.00 G 25608970.00 30645596.00 29026252.00 19334422.00 39490608.00 33953624.00 23858822.00 24264230.00 34411900.00 29471676.00 26684530.00 42157556.00 H 27415780.00 28969036.00 28938988.00 31992140.00 37195488.00 37420412.00 20824092.00 27399478.00 35375084.00 29585522.00 34042840.00 32614114.00 Load time: 07/15/2010 10:25:14 AM Read time: 07/15/2010 10:25:14 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #926 Comment: Plate 2 (316) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 39155120 cps Min counts: 3677045 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 32879290.00 33871124.00 33256418.00 33708600.00 33617596.00 30673746.00 36321880.00 34479240.00 31686838.00 32100618.00 30636686.00 24159966.00 B 31063132.00 33882388.00 30084702.00 38938100.00 32950474.00 32163148.00 36195560.00 35639060.00 34631284.00 30729420.00 26720268.00 25556070.00 C 32154712.00 32149370.00 30551054.00 29912212.00 39155120.00 34574656.00 32517434.00 35874664.00 36178824.00 31462320.00 24941280.00 24263834.00 D 34441100.00 32921210.00 33795880.00 33484914.00 38856836.00 35150608.00 37968476.00 37463340.00 37116604.00 30010820.00 30946664.00 27476174.00 E 32581226.00 33875612.00 35009512.00 32772942.00 37615140.00 38595320.00 32478832.00 35409424.00 35143352.00 30693100.00 29294104.00 28594490.00 F 33988464.00 34521580.00 32283274.00 29241290.00 38034140.00 35796504.00 31929832.00 33581336.00 33566008.00 30683930.00 28070658.00 22689870.00 G 32832286.00 33140450.00 24428464.00 22107474.00 34209436.00 32773002.00 33780756.00 35619000.00 26270914.00 30519560.00 24212288.00 24367240.00 H 32619984.00 33324322.00 31250732.00 30560170.00 34441196.00 36703912.00 35318104.00 33652980.00 31419792.00 32453862.00 26516022.00 27118538.00 Load time: 07/15/2010 10:27:43 AM Read time: 07/15/2010 10:27:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #927 Comment: Plate 3 (317) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 39069748 cps Min counts: 3677045 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 39069748.00 38047796.00 25757590.00 29757264.00 29619840.00 29589784.00 29273322.00 34806664.00 30421188.00 25062936.00 28094248.00 27864406.00 B 29833508.00 25130302.00 24453514.00 23037126.00 28289710.00 25079344.00 37723424.00 29118932.00 30568096.00 23100026.00 26924750.00 26800818.00 C 26606118.00 24119698.00 27149208.00 29754038.00 29110932.00 30740468.00 27454694.00 32891230.00 32239542.00 24849384.00 24460250.00 25825322.00 D 29588476.00 28986254.00 30243786.00 32619380.00 32872910.00 28506706.00 32875762.00 26522332.00 29497588.00 25266462.00 29678604.00 28755878.00 E 25819392.00 30466736.00 23558662.00 31507236.00 31737220.00 32754930.00 31272688.00 30572266.00 28977210.00 19347320.00 31565552.00 30527388.00 F 26797830.00 7804494.00 26699374.00 31486566.00 34618140.00 30706530.00 26178036.00 15703911.00 29108858.00 26184840.00 30812594.00 28975176.00 G 25199084.00 18062524.00 24866694.00 33093740.00 33207882.00 27772438.00 25288494.00 25862140.00 27596644.00 25206014.00 27509104.00 27013322.00 H 27333714.00 28176054.00 27033838.00 35572240.00 34294684.00 32141724.00 28519706.00 29265610.00 20637976.00 26843452.00 30403328.00 24058782.00 Load time: 07/15/2010 10:30:05 AM Read time: 07/15/2010 10:30:05 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #928 Comment: Plate 4 (318) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 40835644 cps Min counts: 3677045 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 32473870.00 24206762.00 27757992.00 26547512.00 27426024.00 27284122.00 31160688.00 25115230.00 27996218.00 29140716.00 32528510.00 32090002.00 B 29327492.00 32267326.00 40835644.00 28913388.00 26004710.00 23675964.00 30911464.00 29607700.00 34740300.00 27392660.00 29053154.00 31777536.00 C 20491830.00 27704470.00 30861886.00 27816134.00 25633620.00 24306354.00 28378024.00 29956734.00 28292842.00 25098894.00 28363508.00 29064912.00 D 31589330.00 32636258.00 33330122.00 24641410.00 29809998.00 26999518.00 25777760.00 31750098.00 28808210.00 27978940.00 30307650.00 32105046.00 E 29304540.00 30372064.00 26251860.00 23301536.00 29176532.00 30112590.00 28722050.00 29167414.00 27214232.00 28305236.00 33570912.00 29392802.00 F 33237538.00 29611516.00 14352572.00 25338524.00 32346348.00 24635520.00 27774072.00 32975178.00 34647368.00 26665410.00 32859984.00 29967360.00 G 28821692.00 28913842.00 22624032.00 20270172.00 31233718.00 23435418.00 28766226.00 29084960.00 23826374.00 27404154.00 28172860.00 27399152.00 H 32147064.00 30185268.00 29860012.00 32571444.00 30861704.00 26636728.00 28441350.00 31653236.00 30541514.00 28914776.00 24436078.00 30149256.00 Load time: 07/15/2010 10:32:28 AM Read time: 07/15/2010 10:32:28 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #929 Comment: Plate 5 (319) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 73766040 cps Min counts: 1031072 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22427656.00 21036968.00 17120246.00 22621288.00 22972430.00 22048802.00 6380788.00 5706514.00 4940133.00 5732637.00 5373281.00 2062144.00 B 21747550.00 19203106.00 18712900.00 13511307.00 21823590.00 18287808.00 5573023.00 6730448.00 3489602.00 5998181.00 4633562.00 4912214.00 C 22086254.00 21049276.00 19731764.00 20675794.00 17780246.00 21788066.00 6646871.00 3266610.00 2835168.00 3040565.00 5057823.00 4423856.00 D 23492432.00 20269154.00 23562392.00 23230566.00 22085420.00 23395832.00 14744275.00 26512482.00 8722636.00 7987919.00 5610189.00 4121684.00 E 21519638.00 19389446.00 24758248.00 24202124.00 22979250.00 23865512.00 7838704.00 17345200.00 3291925.00 8544289.00 4290904.00 6723087.00 F 21948422.00 17622534.00 20956186.00 21598476.00 19026448.00 23009174.00 6377556.00 4841384.00 3967547.00 5359636.00 7584593.00 8227188.00 G 19862804.00 26839592.00 22627054.00 17697802.00 22034308.00 19832164.00 8880647.00 7202557.00 6002400.00 4433731.00 3344891.00 73766040.00 H 15524346.00 17055876.00 16842898.00 14405201.00 12879179.00 16722715.00 10309892.00 13139030.00 18416214.00 11888523.00 12669764.00 12776067.00 Load time: 07/15/2010 10:35:21 AM Read time: 07/15/2010 10:35:21 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #930 Comment: Plate 6 (334) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 5604354 cps Min counts: 877383 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4795600.00 4805565.00 4944532.00 5025057.00 4278785.00 4228154.00 4559770.00 3949951.00 4009201.00 3680547.00 3958839.00 4263973.00 B 4082454.00 4200504.00 3667979.00 2956987.00 4204544.00 4543162.00 3865656.00 3953991.00 1754766.00 3557829.00 3207540.00 4756460.00 C 4735094.00 4377264.00 4569465.00 4612197.00 3131144.00 4862750.00 4688592.00 3635661.00 2888402.00 3895729.00 3235010.00 4613454.00 D 4875946.00 5214654.00 5533074.00 5339886.00 4476552.00 4970027.00 4441541.00 3970868.00 3995555.00 4584906.00 4412455.00 5202266.00 E 4461650.00 4508600.00 4793625.00 5586579.00 4957997.00 5101811.00 4592537.00 4384626.00 3616988.00 4601783.00 3027907.00 5108814.00 F 4870650.00 3566357.00 4964640.00 4780070.00 3168759.00 5604354.00 4841474.00 4052111.00 4440912.00 3982898.00 2214217.00 5235840.00 G 4751612.00 4801256.00 4239824.00 4961588.00 3946450.00 5281444.00 5475980.00 4305627.00 3173157.00 4109745.00 4407966.00 4267833.00 H 5297244.00 4673241.00 3915389.00 5259630.00 3619053.00 4983133.00 5157919.00 5018234.00 4589125.00 3267687.00 3371463.00 4554204.00 Load time: 07/15/2010 10:38:25 AM Read time: 07/15/2010 10:38:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #931 Comment: Plate 7 (335) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 28651818 cps Min counts: 3677045 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20114348.00 23738192.00 24926690.00 24190054.00 23197752.00 22059210.00 23891424.00 19856612.00 20936938.00 20817256.00 20023502.00 20806052.00 B 21234284.00 20806676.00 20175170.00 14649759.00 21016022.00 23406540.00 22258226.00 20742102.00 13113737.00 18724858.00 19045902.00 20548432.00 C 22529716.00 20660704.00 22829020.00 19223304.00 16437167.00 23872162.00 21451580.00 17745408.00 16555795.00 20459300.00 20257542.00 20792794.00 D 22145114.00 17912814.00 21643454.00 21145608.00 20570472.00 24321914.00 28651818.00 19628696.00 18941708.00 19800188.00 21381378.00 21992742.00 E 20613182.00 15963917.00 21485324.00 24158728.00 22280318.00 24597514.00 23924020.00 23168358.00 17116628.00 21463276.00 15573093.00 22052476.00 F 20354112.00 11493788.00 16999246.00 19353666.00 15198032.00 22231794.00 23060198.00 19678972.00 15051656.00 15638298.00 20221154.00 20505600.00 G 20084530.00 18611920.00 16670099.00 18072322.00 18789282.00 20442238.00 24681306.00 19531474.00 16589334.00 18153282.00 20163198.00 20659824.00 H 18296544.00 17409974.00 15118604.00 17369986.00 18985668.00 21811730.00 20620384.00 19938376.00 18072760.00 18439504.00 18287654.00 18057866.00 Load time: 07/15/2010 10:41:00 AM Read time: 07/15/2010 10:41:00 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #932 Comment: Plate 8 (336) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 13083289 cps Min counts: 2182707 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9886753.00 7683445.00 7050125.00 9303420.00 9020886.00 7771649.00 9037039.00 7772658.00 9321399.00 7182359.00 7768255.00 8606317.00 B 8552530.00 7379647.00 7631461.00 6345777.00 10601063.00 9155249.00 8268077.00 7368522.00 4365415.00 10127700.00 8507623.00 10595097.00 C 9807100.00 8251384.00 10153334.00 10117731.00 6650192.00 12242018.00 12309948.00 7848966.00 9526080.00 8298208.00 8466087.00 10405314.00 D 9228087.00 8712059.00 10701502.00 9506155.00 11122989.00 10425013.00 10079540.00 8799111.00 11080758.00 9412213.00 10562713.00 10618593.00 E 8455335.00 7574181.00 7598948.00 10865067.00 13083289.00 9307188.00 12567723.00 10095444.00 10047185.00 9519780.00 9627937.00 12983784.00 F 8932265.00 5500397.00 8162835.00 9933459.00 5980945.00 12059656.00 10709699.00 10470363.00 12632726.00 8689292.00 9906384.00 11744192.00 G 8230525.00 6996352.00 7354145.00 7510717.00 7808165.00 11570302.00 11149468.00 7730157.00 6347752.00 6675777.00 9246068.00 9212839.00 H 8679215.00 5751220.00 6871390.00 8393696.00 11015664.00 10111849.00 8368685.00 9460071.00 8670296.00 6335363.00 7222307.00 8603172.00