Load time: 7/15/2010 13:10 Read time: 7/15/2010 13:10 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #934 Comment: Standard Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 72406912 cps Min counts: 55333 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 72406912 71371072 64518580 113360 B 43738612 43443584 40952016 113180 C 25954822 25057936 23529046 111834 D 15876020 14746920 13804679 110667 E 8647806 8430163 8084274 113090 F 5164935 5074821 4722624 117399 G 3266802 3144287 2899975 111924 H 1965179 1901184 1786657 111475 Load time: 07/15/2010 01:12:18 PM Read time: 07/15/2010 01:12:18 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #935 Comment: Plate 1 (307 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 25204926 cps Min counts: 3676352 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10562723.00 14501478.00 14453758.00 15403432.00 15853194.00 15998658.00 12491392.00 12539698.00 9282917.00 10279021.00 11530197.00 11277366.00 B 10945851.00 14301005.00 13154208.00 14536365.00 13210941.00 16757828.00 8740350.00 12354522.00 10703948.00 11857657.00 10868763.00 21756772.00 C 14987175.00 14872782.00 15717093.00 14367740.00 16792028.00 16561431.00 7725921.00 12058527.00 11454439.00 11523002.00 12149741.00 13780325.00 D 15537256.00 14567117.00 15371629.00 16258005.00 14158289.00 15700917.00 8804576.00 11207446.00 11158633.00 12527944.00 10788553.00 11831673.00 E 15115916.00 15683911.00 15188948.00 17301574.00 17922124.00 19201594.00 10070117.00 10727946.00 12581992.00 12992919.00 25204926.00 13120563.00 F 15859471.00 15434201.00 16746066.00 14048696.00 15760913.00 18595660.00 11268110.00 14057854.00 14532915.00 12700280.00 12602204.00 10603550.00 G 13781658.00 12903466.00 15103671.00 15549599.00 17375742.00 16580395.00 12642428.00 11039777.00 11931054.00 12152633.00 13025949.00 11820560.00 H 11781324.00 11950977.00 10700558.00 11812808.00 11871008.00 9758878.00 11223047.00 11114171.00 12910876.00 13494372.00 13345809.00 13254213.00 Load time: 07/15/2010 01:14:43 PM Read time: 07/15/2010 01:14:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #936 Comment: Plate 2 (311) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 65046312 cps Min counts: 3676352 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26153424.00 24293982.00 26764476.00 26876104.00 27584588.00 29139818.00 29801484.00 28346774.00 29242244.00 24108780.00 23472862.00 24047984.00 B 24114028.00 22777658.00 23311122.00 17697864.00 29926660.00 30117448.00 30244080.00 25557844.00 65046312.00 21784696.00 21701470.00 26085300.00 C 22654436.00 24360258.00 25082862.00 28377912.00 22612712.00 33217764.00 32011318.00 27545236.00 22487370.00 21696294.00 24117480.00 25197324.00 D 24386304.00 23044924.00 27686880.00 28053184.00 28719708.00 34716436.00 29213640.00 26809994.00 27097798.00 24694820.00 26371040.00 27228612.00 E 23941210.00 19491636.00 30517938.00 29152550.00 31334348.00 34076588.00 28014232.00 27016690.00 25576604.00 25960598.00 18149522.00 24321274.00 F 20885328.00 16820638.00 22646342.00 22613576.00 23250586.00 33774516.00 28003670.00 27894710.00 23758104.00 21431460.00 24438586.00 34550140.00 G 18791598.00 15769788.00 18906784.00 22427122.00 24956318.00 29812864.00 28703606.00 25795854.00 22688306.00 19330890.00 20486426.00 22470892.00 H 21927754.00 20047522.00 20093134.00 20575908.00 21292130.00 28200500.00 28506282.00 28509088.00 24489736.00 14193059.00 17170880.00 25291624.00 Load time: 07/15/2010 01:17:00 PM Read time: 07/15/2010 01:17:00 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #937 Comment: Plate 3 (312) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 36211196 cps Min counts: 165373 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24684692.00 24512642.00 24525058.00 23867104.00 23798336.00 330746.00 22895338.00 20855304.00 21785470.00 18540112.00 22934286.00 22733190.00 B 24307092.00 21637964.00 20814724.00 19521504.00 27290622.00 22573034.00 22948804.00 18771218.00 10428474.00 20492282.00 22847588.00 24618324.00 C 23289914.00 24621438.00 24872488.00 24388376.00 21006994.00 21660438.00 28600998.00 23635998.00 15777356.00 21361238.00 17658284.00 25385968.00 D 23752238.00 20388118.00 24050376.00 23930786.00 24780814.00 23670300.00 26965638.00 21812654.00 22219772.00 22840416.00 26056164.00 25515060.00 E 24011044.00 24219132.00 23577576.00 23584746.00 27522842.00 26782672.00 23508390.00 23012700.00 22442348.00 22924050.00 16728691.00 20864206.00 F 23361498.00 19950620.00 22053096.00 27460714.00 23387418.00 26560362.00 22450102.00 24657356.00 22964514.00 20662520.00 23687824.00 23754728.00 G 22128002.00 24056420.00 22012388.00 23179370.00 23988966.00 23116042.00 28582924.00 19435076.00 20163092.00 20713420.00 22450528.00 25041534.00 H 24663940.00 25555090.00 19812876.00 23430690.00 24493766.00 14825012.00 36211196.00 15888528.00 24153556.00 21283896.00 21076148.00 23119110.00 Load time: 07/15/2010 01:19:14 PM Read time: 07/15/2010 01:19:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #938 Comment: Plate4 (314) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 29198836 cps Min counts: 2897282 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18443844.00 15008529.00 16773671.00 14812946.00 24288206.00 14628325.00 15930669.00 14855931.00 12751312.00 25064650.00 23398030.00 25005408.00 B 17676514.00 16890726.00 16565154.00 17134068.00 29198836.00 14798076.00 17624854.00 14476777.00 19106708.00 21253294.00 21275178.00 21444648.00 C 16408986.00 19769896.00 19633022.00 16405477.00 25951926.00 15692487.00 15288216.00 14951690.00 17601086.00 23267540.00 22343812.00 20225174.00 D 17571370.00 18029008.00 22322880.00 15102101.00 28662858.00 17042732.00 14071561.00 18601626.00 19400634.00 23183596.00 24857892.00 26296268.00 E 18452954.00 19540162.00 19999884.00 14205898.00 24849390.00 16599313.00 13381873.00 13726327.00 16210460.00 21828946.00 24134848.00 22442444.00 F 17274412.00 17630594.00 18517598.00 13491867.00 28760128.00 14101998.00 10995077.00 13153846.00 15433554.00 21025186.00 19613950.00 19628642.00 G 16795404.00 17466094.00 16476990.00 8790850.00 24992164.00 13959722.00 10893181.00 5794565.00 9010857.00 16795212.00 18100838.00 16701585.00 H 19384710.00 19937052.00 14755628.00 14448937.00 15077468.00 13387106.00 11830553.00 12008522.00 9071707.00 18522348.00 21435632.00 19243346.00 Load time: 07/15/2010 01:21:38 PM Read time: 07/15/2010 01:21:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #939 Comment: Plate 5 (337) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 58451388 cps Min counts: 1328594 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11804483.00 10245174.00 11642663.00 10399022.00 11098875.00 11334484.00 11264716.00 9921669.00 10744488.00 2657188.00 10741855.00 10474279.00 B 9086976.00 9864986.00 8806692.00 8341119.00 11410623.00 13455782.00 10472038.00 10160298.00 6379675.00 6589073.00 9665656.00 10619946.00 C 9794055.00 9897333.00 9838600.00 8323280.00 8759338.00 13620989.00 12054745.00 10700397.00 9706803.00 9698297.00 9990067.00 10612321.00 D 9674978.00 8318285.00 10484472.00 11868658.00 11902731.00 15621028.00 56240684.00 11877508.00 11082478.00 10122752.00 9559377.00 10959840.00 E 9328491.00 7304059.00 10405798.00 12084424.00 13232273.00 16562767.00 10702379.00 11526820.00 11060375.00 10983790.00 9020719.00 10837216.00 F 9464939.00 6162020.00 6938757.00 9590641.00 58451388.00 15273563.00 11646536.00 11333303.00 11469147.00 8719207.00 10423340.00 11315201.00 G 8181371.00 8101493.00 6197204.00 7541757.00 9387383.00 11788183.00 9989749.00 9428541.00 7950263.00 7901901.00 8868047.00 9438302.00 H 9828162.00 9453120.00 7770412.00 9083532.00 10889401.00 11653698.00 9308572.00 11274949.00 10250956.00 8249373.00 10310472.00 10429474.00 Load time: 07/15/2010 01:24:21 PM Read time: 07/15/2010 01:24:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #940 Comment: Plate 6 (338) Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 16716218 cps Min counts: 613473 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9756646.00 12458593.00 9828968.00 11894287.00 14217236.00 13534444.00 13625511.00 2657098.00 3818793.00 5316351.00 2990268.00 3437067.00 B 13521177.00 12158766.00 13894951.00 10383395.00 13459689.00 14266089.00 14077768.00 3169059.00 1226947.00 3633988.00 3075176.00 4324471.00 C 14215510.00 13422769.00 14213422.00 12570931.00 11181601.00 14416921.00 13771190.00 2936236.00 2797654.00 3297498.00 3582469.00 5085771.00 D 13752315.00 14139736.00 14204487.00 16003617.00 14291611.00 14562396.00 11551192.00 3638656.00 3307910.00 3408704.00 3740976.00 4172337.00 E 14225423.00 11842051.00 16716218.00 13999152.00 14157509.00 15748873.00 13059987.00 3683354.00 3091601.00 3979544.00 2506669.00 3464173.00 F 14201216.00 10269161.00 13569457.00 12616450.00 12045317.00 14690472.00 13060135.00 3551504.00 3397036.00 2831761.00 3811344.00 4205098.00 G 12842107.00 12989607.00 11640095.00 10370628.00 11644024.00 12672717.00 11416179.00 3362660.00 2228878.00 2965226.00 4803852.00 3370828.00 H 4808878.00 4594184.00 4566630.00 3761440.00 3772659.00 4271516.00 3162148.00 5143035.00 3464442.00 2829607.00 3155416.00 3811433.00