Load time: 07/23/2010 12:29:42 PM Read time: 07/23/2010 12:29:42 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #960 Comment: Stan Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 102538888 cps Min counts: 61915 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 98864728.00 102538888.00 98673096.00 127419.00 B 58937368.00 56553676.00 51492468.00 127419.00 C 33638112.00 31950132.00 30857846.00 123830.00 D 19453136.00 18889398.00 18017490.00 124548.00 E 11454552.00 10717715.00 10400285.00 126612.00 F 6867841.00 6197541.00 5993848.00 125176.00 G 4166809.00 3682075.00 3505930.00 127868.00 H 2545077.00 2197274.00 2135898.00 125176.00 Load time: 07/23/2010 12:31:28 PM Read time: 07/23/2010 12:31:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #961 Comment: T12:9 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 41960320 cps Min counts: 3675434 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23843096.00 25540490.00 26614934.00 32519752.00 26166922.00 41960320.00 15255768.00 17237466.00 15938924.00 18753940.00 15211010.00 18414832.00 B 27693292.00 29248322.00 31130656.00 27770168.00 30129108.00 34775184.00 21710366.00 17942902.00 16091392.00 15958904.00 10348259.00 16681323.00 C 23107062.00 27418548.00 25172174.00 28525176.00 36554940.00 25408066.00 13342742.00 17387470.00 22155892.00 11845171.00 16264244.00 16622246.00 D 16617511.00 26923520.00 21116306.00 22835126.00 37951524.00 35014144.00 19271738.00 19569192.00 22996474.00 26194484.00 19482606.00 16225538.00 E 17253682.00 20320482.00 25932286.00 22440250.00 25294796.00 30607018.00 13421851.00 19710868.00 16376066.00 20827472.00 22116938.00 13473169.00 F 17060306.00 17659212.00 16849884.00 22751432.00 23202768.00 27545828.00 12029556.00 12495707.00 22224864.00 15956091.00 15087688.00 15139089.00 G 18394904.00 12570991.00 14719437.00 21389186.00 21425370.00 23976222.00 18898120.00 16559212.00 19212414.00 9626700.00 13124097.00 15108433.00 H 23895008.00 17707032.00 17689502.00 19623804.00 30801830.00 25251138.00 15847958.00 13518943.00 10421084.00 11264682.00 14477192.00 16925086.00 Load time: 07/23/2010 12:33:46 PM Read time: 07/23/2010 12:33:46 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #962 Comment: T12:10 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 18770662 cps Min counts: 3260423 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10219684.00 8569228.00 9865996.00 10419256.00 10434070.00 10111654.00 11042557.00 9621057.00 9484772.00 10462089.00 9964461.00 9525948.00 B 8649202.00 9466080.00 9497179.00 12885293.00 13913669.00 11013445.00 15552294.00 12836654.00 12758549.00 13026712.00 8384825.00 10056341.00 C 11932291.00 10940903.00 13594705.00 12815890.00 13729026.00 13140984.00 10881816.00 14321367.00 16743256.00 15824234.00 10185616.00 12403177.00 D 11301678.00 11317526.00 13610675.00 14065435.00 11593294.00 12573455.00 11900772.00 12325211.00 17115424.00 18770662.00 18173394.00 12425567.00 E 8558736.00 13418912.00 15191746.00 12900104.00 11310968.00 13948481.00 15876457.00 14635293.00 18150124.00 14158935.00 11508535.00 10776871.00 F 7559020.00 11001198.00 9250540.00 11714346.00 13079495.00 12168923.00 14662460.00 14326940.00 15979221.00 14948211.00 13433223.00 12156932.00 G 7457666.00 6715386.00 6520846.00 9631623.00 10560082.00 11904384.00 16898512.00 14694914.00 12059850.00 11437044.00 10970385.00 10426383.00 H 8656157.00 8159503.00 7013835.00 9089749.00 8776816.00 9512607.00 12040436.00 10596440.00 8167171.00 9422294.00 14552569.00 10207674.00 Load time: 07/23/2010 12:36:27 PM Read time: 07/23/2010 12:36:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #963 Comment: T12:11 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 34373920 cps Min counts: 3675434 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15242221.00 14514782.00 14549665.00 15290560.00 17466978.00 16953384.00 21480630.00 20596730.00 19502764.00 22278074.00 20360624.00 19486354.00 B 14174399.00 13522087.00 13926516.00 18122810.00 17590180.00 21466276.00 33601760.00 29729670.00 24562518.00 22009250.00 19179258.00 21736878.00 C 13964142.00 15815389.00 14746710.00 17824722.00 17740390.00 15861616.00 26081230.00 27316008.00 30782484.00 29933272.00 20132418.00 23133998.00 D 13100203.00 13355868.00 19180950.00 18560748.00 21581458.00 19740988.00 27328824.00 34373920.00 30067290.00 25056360.00 29214354.00 22245632.00 E 12887377.00 15701190.00 15816899.00 17939792.00 18269194.00 19082422.00 30772820.00 28087522.00 33263356.00 25444972.00 27904386.00 23444414.00 F 12924019.00 12272758.00 11851878.00 15735430.00 16612389.00 16788580.00 22801638.00 24118276.00 24018068.00 20079164.00 23224630.00 21312946.00 G 12399206.00 11872271.00 11284759.00 13707894.00 14158892.00 15642824.00 21063662.00 20312812.00 19353326.00 19677690.00 22934156.00 20114646.00 H 12665204.00 13095930.00 10799895.00 14877025.00 15035097.00 15395380.00 19986798.00 19764300.00 18621214.00 19293376.00 23909496.00 19808944.00 Load time: 07/23/2010 12:38:41 PM Read time: 07/23/2010 12:38:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #964 Comment: T12:12 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 62390984 cps Min counts: 3675434 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35561336.00 44254348.00 39792420.00 44713536.00 48255964.00 41327572.00 38352372.00 39479540.00 36433728.00 37270164.00 39045660.00 32354150.00 B 35785352.00 35030628.00 38907320.00 51631644.00 60069764.00 56625996.00 48809252.00 44801664.00 43344484.00 34791216.00 40694152.00 39239296.00 C 41991672.00 42552116.00 45743720.00 48467868.00 47713024.00 54784708.00 43217424.00 45156308.00 56362792.00 47311900.00 37673772.00 42597236.00 D 38553604.00 48746848.00 43572632.00 55617284.00 55169060.00 48711576.00 45811964.00 48483468.00 57548800.00 52758392.00 59827864.00 39600940.00 E 39175092.00 48807572.00 47677932.00 52046268.00 49047588.00 62390984.00 51592220.00 52022180.00 57744816.00 51707624.00 53141784.00 38761580.00 F 37764428.00 39893828.00 34912072.00 40129564.00 45803232.00 62379028.00 43557816.00 43834440.00 54154676.00 49317280.00 47482004.00 38866592.00 G 30708874.00 31763394.00 35511376.00 33853848.00 39343080.00 43424372.00 47778772.00 50125872.00 43378348.00 35625828.00 51822664.00 39362380.00 H 33889812.00 31281918.00 30843188.00 38684264.00 36997676.00 43082924.00 41146164.00 37906240.00 34337760.00 36518364.00 44968632.00 38506508.00 Load time: 07/23/2010 12:40:53 PM Read time: 07/23/2010 12:40:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #965 Comment: T12:13 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 16915908 cps Min counts: 3650534 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9709345.00 8893490.00 9880425.00 10222057.00 11039692.00 10190501.00 9775872.00 10065122.00 10491799.00 10557258.00 10491009.00 10629153.00 B 9631857.00 8927857.00 9120006.00 10757828.00 11627060.00 10575009.00 11753614.00 12109258.00 11884791.00 11708348.00 7379862.00 9832960.00 C 10196472.00 9918651.00 11431608.00 10056745.00 13108216.00 13256547.00 12782102.00 13692794.00 14082396.00 12798313.00 10158113.00 13897779.00 D 9594808.00 10601789.00 11342817.00 12293451.00 11250751.00 12672029.00 15369771.00 14488406.00 14961769.00 16915908.00 16287395.00 13980672.00 E 8525946.00 9978573.00 12442679.00 11513348.00 11092206.00 12650644.00 12466970.00 14821334.00 15017969.00 13451103.00 13240886.00 11781939.00 F 8191144.00 8797810.00 8220371.00 10635148.00 10237004.00 10267831.00 11597698.00 13845750.00 12575434.00 12773768.00 13167828.00 13409389.00 G 8237840.00 7827233.00 7301068.00 8276921.00 10088858.00 11233111.00 12594088.00 13481239.00 11819042.00 11326025.00 11860755.00 11052357.00 H 9398187.00 8096375.00 7345755.00 9227564.00 9061883.00 10200515.00 7595503.00 12497110.00 10896300.00 11121731.00 13641940.00 10713158.00 Load time: 07/23/2010 12:43:39 PM Read time: 07/23/2010 12:43:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #966 Comment: T12:14 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 33881372 cps Min counts: 1056867 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18668656.00 16518451.00 19243788.00 18657210.00 11577798.00 18256316.00 2711261.00 2642257.00 2453281.00 2471766.00 2307466.00 2666574.00 B 20548202.00 16191535.00 17882108.00 28157818.00 25035178.00 28146024.00 3158666.00 3484843.00 2969242.00 2571997.00 2113734.00 3308878.00 C 18073370.00 23384196.00 25028138.00 23758542.00 26898080.00 24250336.00 4409983.00 2938374.00 3081586.00 2996700.00 2753615.00 4103817.00 D 18884148.00 25651700.00 28088508.00 25447404.00 31011800.00 22556828.00 4081025.00 4705203.00 4302664.00 2772728.00 4872015.00 3751887.00 E 19209632.00 22438168.00 26230638.00 22631944.00 32046326.00 33881372.00 5299590.00 4726021.00 2993918.00 4010226.00 4026108.00 3587856.00 F 20830778.00 20214544.00 19885822.00 24323744.00 24292638.00 20918000.00 3194200.00 3460616.00 3563269.00 2326130.00 3829864.00 3882447.00 G 21188092.00 20648100.00 17810108.00 21324608.00 20126816.00 21175554.00 3058076.00 3147001.00 3172037.00 2743834.00 3782216.00 4894269.00 H 18852346.00 23254826.00 17174462.00 20435566.00 19615020.00 21763470.00 3160461.00 2958474.00 2676266.00 2919081.00 4431968.00 4124814.00 Load time: 07/23/2010 12:46:07 PM Read time: 07/23/2010 12:46:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #967 Comment: T12:15 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 14863657 cps Min counts: 1789082 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10106038.00 6895299.00 8517598.00 8588401.00 8762722.00 8528666.00 6464046.00 8076395.00 4317290.00 5606026.00 5861135.00 6207950.00 B 7314977.00 8586569.00 6608874.00 7645337.00 11062269.00 9494982.00 7909424.00 6723372.00 7654170.00 5940547.00 6013679.00 3578165.00 C 7987222.00 7638167.00 5100833.00 9634347.00 10159067.00 10268410.00 10757371.00 10448473.00 9026899.00 9162474.00 5713884.00 6802875.00 D 7386976.00 7340192.00 12098133.00 10253073.00 14863657.00 12540388.00 9092868.00 9427441.00 10499844.00 7204786.00 10257526.00 7384168.00 E 7385293.00 8727734.00 9973499.00 8505188.00 12622794.00 10888498.00 10732383.00 11338786.00 12486083.00 11771298.00 7514347.00 8983581.00 F 6855099.00 6723551.00 6949497.00 9832686.00 11531760.00 12332473.00 10984667.00 9075102.00 10291160.00 7960629.00 8312716.00 7750747.00 G 7493699.00 6029113.00 7004593.00 6323973.00 9802380.00 10656522.00 5665967.00 6796235.00 5773646.00 6118128.00 6940255.00 6446279.00 H 7841169.00 6706771.00 5870018.00 7173559.00 6294093.00 8748308.00 5959032.00 6605015.00 5246558.00 5171272.00 7467186.00 6689453.00 Load time: 07/23/2010 12:48:46 PM Read time: 07/23/2010 12:48:46 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #968 Comment: T12:16 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 38357180 cps Min counts: 3675434 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20440270.00 20698510.00 21227078.00 21762300.00 25486664.00 24797158.00 27017824.00 23419464.00 17735716.00 16319814.00 25183956.00 19360512.00 B 19457604.00 16660182.00 26487614.00 24963286.00 27810028.00 24004752.00 31389986.00 25257612.00 24753118.00 17523798.00 22857566.00 16168258.00 C 25730948.00 20940274.00 28738782.00 28169560.00 29693812.00 27285998.00 25954184.00 30672328.00 29759396.00 24773526.00 20435998.00 23546258.00 D 19586386.00 20307964.00 26425380.00 28336398.00 27421740.00 28990214.00 38357180.00 35407824.00 35199176.00 27658032.00 29054384.00 23554470.00 E 23091790.00 25484510.00 27260194.00 27629716.00 26726934.00 32243284.00 35869588.00 22678740.00 35811988.00 26021050.00 25407806.00 21339376.00 F 18198906.00 18577062.00 24025920.00 25493704.00 27869136.00 23997480.00 32352212.00 22416570.00 32772312.00 24817298.00 21630890.00 24831686.00 G 18528870.00 16805160.00 18665500.00 24036226.00 29472702.00 27581634.00 22900444.00 23913168.00 22871246.00 22547048.00 19540622.00 20691450.00 H 17787698.00 19323442.00 15990530.00 20672084.00 23352076.00 20811798.00 24229092.00 20883198.00 23930966.00 20554512.00 26732006.00 21692812.00