Load time: 7/27/2010 13:40 Read time: 7/27/2010 13:40 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #973 Comment: Standard Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 94687632 cps Min counts: 55420 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 94687632 91901288 116405 117393 B 52284984 50840048 114969 120175 C 31464296 29947784 114969 116854 D 17618124 17385894 114072 118200 E 9990038 9987144 113444 117572 F 5829088 5722375 114790 117752 G 3434376 3377295 112636 116764 H 1947756 1939588 110841 114790 Load time: 7/27/2010 13:42 Read time: 7/27/2010 13:42 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #974 Comment: T12:3. Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 61934760 cps Min counts: 3676162 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 50640588 46374560 44755600 48746160 51113960 49040932 48917404 44334000 42784624 39392872 46486920 42012276 B 51301208 47305376 35241144 47172380 49009420 54066060 54522484 47758436 29626164 34959432 38115840 40057512 C 59251844 46086660 49963876 55465300 40197548 52201684 46377428 45199792 42183900 42078920 39084788 42818564 D 55144720 47094120 51573476 54093744 49078708 61934760 51363064 48931204 47199996 50151188 50548464 39882076 E 60056140 47412568 28676042 54964816 54678756 58093864 52354012 48410428 46051268 42172676 33240312 39756736 F 55528132 41902424 46394984 48288344 43526564 57391432 49530616 45380012 43647960 36228768 38788928 40966004 G 56797344 46329676 44147500 44279960 46592148 47093940 48168376 42775816 38766264 37425288 42680564 36806748 H 48575472 48190856 43806136 44005976 44704772 51130240 34783748 44696828 38203936 35194820 34627760 42293144 Load time: 07/27/2010 01:44:43 PM Read time: 07/27/2010 01:44:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #975 Comment: T12:10 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 24300916 cps Min counts: 3676162 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17152024.00 16622319.00 17671742.00 17984258.00 18097158.00 18361442.00 18793468.00 20228692.00 18773700.00 19408554.00 17197350.00 20675596.00 B 15014052.00 15675195.00 15781333.00 17714934.00 18745638.00 18663812.00 18071314.00 21751024.00 19248646.00 18853728.00 16117701.00 19070346.00 C 16964982.00 19168732.00 16787194.00 17524286.00 17876384.00 20350484.00 19331354.00 23622930.00 21183238.00 19359960.00 16248720.00 20432170.00 D 15317681.00 16714540.00 17822452.00 20043576.00 18955248.00 20598336.00 18976708.00 23796396.00 23129336.00 22451972.00 22405282.00 21081898.00 E 15373604.00 16854950.00 18106846.00 19496334.00 18633056.00 19169484.00 18948694.00 24300916.00 23368520.00 20803104.00 19468642.00 20166150.00 F 14667042.00 14122987.00 13632119.00 15826059.00 16978216.00 19369234.00 19191226.00 23139856.00 21495812.00 19528294.00 21761222.00 20422840.00 G 14494839.00 13057118.00 13060969.00 14710663.00 15435127.00 18442278.00 18168512.00 18884974.00 18462416.00 20649418.00 19769638.00 17351838.00 H 16166217.00 14602705.00 13704261.00 15666671.00 16740959.00 17351462.00 18116260.00 18051800.00 18088066.00 18921640.00 21874718.00 18606612.00 Load time: 07/27/2010 01:47:07 PM Read time: 07/27/2010 01:47:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #976 Comment: T12:13 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 14585439 cps Min counts: 3448332 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9938791.00 8780335.00 8366178.00 8322677.00 10324974.00 9930051.00 10094864.00 9620828.00 9761075.00 9838824.00 9575285.00 12229012.00 B 10052904.00 8411992.00 8092721.00 7795747.00 9627628.00 11927970.00 10218841.00 9080151.00 9538901.00 8803971.00 9387995.00 13139818.00 C 8981925.00 9966397.00 9436616.00 8390014.00 10569538.00 11371070.00 11967870.00 10762463.00 11653717.00 9814168.00 9600227.00 13690297.00 D 9210605.00 8595922.00 10070139.00 9604494.00 10627327.00 13342106.00 12791679.00 13956863.00 14585439.00 12613836.00 13440545.00 13545142.00 E 10013915.00 8153846.00 9784728.00 10256489.00 9635054.00 11930618.00 13681767.00 12172792.00 12588804.00 12606404.00 13011225.00 14066412.00 F 9729164.00 8032043.00 7293719.00 7846905.00 8852736.00 9986676.00 9889891.00 9812105.00 10518510.00 10249062.00 11609397.00 13681733.00 G 9255945.00 7708130.00 7529146.00 6896664.00 8793700.00 9635940.00 9913023.00 10889322.00 9453279.00 10275421.00 10547590.00 11113113.00 H 10894739.00 8352820.00 6903037.00 8357860.00 7768361.00 9546992.00 9429660.00 9889885.00 9223504.00 10354709.00 10801350.00 11858194.00 Load time: 07/27/2010 01:49:52 PM Read time: 07/27/2010 01:49:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #977 Comment: T12:14 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 24488242 cps Min counts: 3676162 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16403325.00 16680629.00 17433064.00 17448842.00 17797662.00 19509620.00 18067602.00 18323550.00 16133666.00 15671840.00 17374562.00 19767236.00 B 14817547.00 16133121.00 15317426.00 18408848.00 18736974.00 21147898.00 20541364.00 19146230.00 18868284.00 16075314.00 16519614.00 18906926.00 C 17423214.00 16699810.00 17457146.00 18296792.00 19870546.00 22983496.00 19551828.00 20729376.00 19648314.00 18323304.00 16816268.00 20249932.00 D 17981434.00 16990418.00 17527652.00 19464716.00 19769906.00 22122674.00 24488242.00 22291444.00 20949084.00 21601880.00 22160120.00 17299660.00 E 14818127.00 16405096.00 17964106.00 18855682.00 19075276.00 21619994.00 21624978.00 21052670.00 20199526.00 20411446.00 20229594.00 18879116.00 F 13976900.00 14818442.00 14966515.00 17657974.00 17002236.00 21266100.00 19715228.00 19345078.00 18647234.00 19504102.00 20785534.00 20272232.00 G 14528603.00 13602378.00 12873669.00 14305793.00 16178620.00 19269340.00 19681208.00 18611124.00 16067220.00 17142280.00 18190556.00 15983328.00 H 14681331.00 13785886.00 11835917.00 14995943.00 16062529.00 18288344.00 18944564.00 18304864.00 16056454.00 17766052.00 20400534.00 19590620.00 Load time: 07/27/2010 01:52:17 PM Read time: 07/27/2010 01:52:17 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #978 Comment: T12:15 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 10874218 cps Min counts: 1863929 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9768887.00 8029811.00 8899286.00 9377901.00 9405794.00 10190174.00 6482737.00 6316251.00 3727858.00 6187100.00 5687911.00 6268683.00 B 7987410.00 7829211.00 7296142.00 8522031.00 9030294.00 10170845.00 6451683.00 6410219.00 5607674.00 5869924.00 5491807.00 6986594.00 C 8252739.00 9150637.00 6332854.00 8783145.00 9795833.00 10857621.00 6887599.00 7702407.00 7930404.00 6825582.00 5821280.00 7320464.00 D 8619028.00 7508581.00 9559232.00 9365608.00 9944600.00 9646368.00 7913331.00 8553139.00 7681118.00 7309784.00 8826958.00 7988479.00 E 7358234.00 8523329.00 9428402.00 10077940.00 9966183.00 10270185.00 7482097.00 7580487.00 6805747.00 7257190.00 7264729.00 8182162.00 F 6886163.00 6563871.00 8084972.00 7507934.00 8732626.00 10874218.00 6431220.00 6531651.00 6662327.00 5860590.00 7444238.00 7442974.00 G 6266170.00 6351612.00 5411301.00 6986953.00 10200474.00 9274926.00 7781158.00 6491981.00 5431046.00 5450791.00 7692882.00 6403218.00 H 7609125.00 8015716.00 6119698.00 7758958.00 7175877.00 9521467.00 6347394.00 6908063.00 5268060.00 5783226.00 8069793.00 6563961.00