Load time: 07/30/2010 09:28:43 AM Read time: 07/30/2010 09:28:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #980 Comment: Tso7 Stan and Con 2 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 99484832 cps Min counts: 64631 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 99484832.00 96272496.00 95604632.00 132225.00 131507.00 131776.00 130968.00 15029096.00 6843590.00 11892329.00 6703465.00 5904995.00 B 58077916.00 59122748.00 54207840.00 131776.00 131507.00 130430.00 131507.00 12664183.00 6410468.00 10258991.00 6185784.00 134290.00 C 34229820.00 33207016.00 32811846.00 130160.00 129981.00 130699.00 131507.00 12923539.00 5706074.00 9983402.00 5258141.00 134649.00 D 19698274.00 18545194.00 18518936.00 130071.00 130699.00 129801.00 130430.00 10888007.00 6489822.00 6162893.00 6776804.00 133302.00 E 11158794.00 10647436.00 10547339.00 129801.00 129263.00 130879.00 130071.00 12083526.00 6870879.00 7178956.00 6164599.00 129801.00 F 6513430.00 6440450.00 6368637.00 130519.00 130789.00 147306.00 130519.00 10933839.00 9593037.00 4688754.00 7016928.00 131507.00 G 3708867.00 3656892.00 4758502.00 132045.00 132674.00 131058.00 131686.00 10601608.00 9687694.00 6570252.00 6917737.00 132405.00 H 2192268.00 2213902.00 2134189.00 130430.00 131238.00 129891.00 130699.00 5778874.00 13968456.00 5305627.00 5029776.00 133841.00 Load time: 07/30/2010 09:31:25 AM Read time: 07/30/2010 09:31:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #981 Comment: 32 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 45742400 cps Min counts: 3676820 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21030902.00 17401566.00 17434412.00 19149448.00 19059238.00 21913496.00 26075942.00 22269952.00 15765175.00 19094214.00 19168594.00 17066434.00 B 20399364.00 21019242.00 22655932.00 26296754.00 23041070.00 26696884.00 36616028.00 29940046.00 18103586.00 25050246.00 16875562.00 16163958.00 C 19274626.00 23828186.00 25834498.00 24605784.00 25831848.00 26688842.00 36082064.00 33682236.00 28496230.00 32177022.00 21028630.00 21545292.00 D 17181058.00 20092966.00 29832528.00 31006380.00 31194256.00 35843652.00 36854476.00 45742400.00 36113252.00 37691068.00 36459028.00 15942278.00 E 17732640.00 27440676.00 32307790.00 35141364.00 29646206.00 36862348.00 41904952.00 45066476.00 38223916.00 35549424.00 23083338.00 20903388.00 F 15824673.00 24582424.00 23746670.00 29519912.00 30424608.00 34293224.00 38366072.00 39388904.00 38500376.00 36666528.00 28858536.00 23469002.00 G 15778178.00 17959152.00 20718788.00 19281520.00 32968248.00 32255610.00 36158032.00 28857314.00 40355700.00 36382296.00 21965434.00 21840306.00 H 16216884.00 17354796.00 14468481.00 16074648.00 26885986.00 22649822.00 19310360.00 28953210.00 19127480.00 25968630.00 24269536.00 21036304.00 Load time: 07/30/2010 09:33:51 AM Read time: 07/30/2010 09:33:51 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #982 Comment: 75 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 41278128 cps Min counts: 3676820 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18256478.00 16764897.00 17001174.00 17218050.00 19076402.00 19452190.00 18693128.00 15995828.00 19567744.00 15891406.00 17260502.00 16840632.00 B 15024901.00 16192145.00 20276362.00 31656418.00 24524936.00 28534710.00 25056700.00 19267768.00 24254984.00 17109630.00 15447312.00 16954170.00 C 16171827.00 28346838.00 31622170.00 39752844.00 18456792.00 20254390.00 19429660.00 15777967.00 20304444.00 23559966.00 16179057.00 18530108.00 D 15875631.00 26106806.00 33159816.00 32776780.00 33904276.00 24472488.00 25746346.00 19019476.00 24485070.00 25315460.00 33725488.00 16613815.00 E 17180922.00 27533742.00 31733356.00 20538956.00 31228860.00 36842072.00 27615322.00 28250678.00 30855512.00 33257446.00 22948916.00 17551570.00 F 14870752.00 17224092.00 25234652.00 30554540.00 35893896.00 36189744.00 26748448.00 30113656.00 41278128.00 25152844.00 32489786.00 21115958.00 G 13650101.00 16412925.00 25907514.00 21797968.00 29627714.00 35783476.00 39346752.00 33300246.00 26254508.00 22016176.00 25604132.00 17696230.00 H 15424101.00 17200194.00 14096810.00 19959862.00 22267832.00 26249336.00 18076884.00 17078850.00 18574200.00 17520622.00 20271878.00 18364790.00 Load time: 07/30/2010 09:36:10 AM Read time: 07/30/2010 09:36:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #983 Comment: 76 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 36445188 cps Min counts: 3676820 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13538063.00 10390111.00 10710174.00 9922631.00 10307699.00 11849667.00 18247670.00 14874784.00 17936400.00 17759218.00 16937776.00 18435600.00 B 12018312.00 9031509.00 10706791.00 12933461.00 10471231.00 20592640.00 24471560.00 21175960.00 27940318.00 16641238.00 15851431.00 22245564.00 C 12931605.00 9935770.00 12090941.00 14355984.00 12689429.00 13151294.00 27700896.00 21214142.00 29105254.00 28087056.00 12684267.00 21158656.00 D 11680147.00 10525630.00 12450519.00 15130673.00 16284630.00 16775957.00 18973738.00 24327842.00 19807306.00 28591048.00 31060470.00 20642238.00 E 12151814.00 10985897.00 12273832.00 10698427.00 17729004.00 16696548.00 29946726.00 21388202.00 26788086.00 20202498.00 24137232.00 20779206.00 F 11331559.00 9403978.00 13715477.00 13177897.00 16442622.00 22141394.00 36445188.00 29135302.00 27664670.00 32876482.00 21950980.00 21134480.00 G 10449225.00 8982243.00 11674464.00 10737814.00 15386733.00 14809973.00 35451432.00 26386054.00 33023824.00 29401816.00 23627568.00 22549528.00 H 11410199.00 9661703.00 9990967.00 11371888.00 10871878.00 13074310.00 22080868.00 21082646.00 22215092.00 25112012.00 23764932.00 18975334.00 Load time: 07/30/2010 09:38:34 AM Read time: 07/30/2010 09:38:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #984 Comment: perin os11 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 22593508 cps Min counts: 69209 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12950089.00 12454961.00 13788647.00 13903339.00 14804159.00 10713282.00 12108194.00 14341057.00 12488962.00 14700778.00 14195538.00 19542118.00 B 11648386.00 12638538.00 8893709.00 11426588.00 10190093.00 7571632.00 9874411.00 10448747.00 13668746.00 16253315.00 12917045.00 22327548.00 C 12548205.00 10802335.00 10309701.00 10957635.00 10362462.00 11682005.00 12189618.00 11771192.00 14814073.00 12031827.00 16857168.00 21343222.00 D 12032406.00 11337835.00 10093558.00 10119832.00 10064971.00 10570362.00 12541402.00 10027928.00 13051889.00 12504565.00 17724996.00 22593508.00 E 13204380.00 10322553.00 11421693.00 10772607.00 9048523.00 11003834.00 10688434.00 13551716.00 11730352.00 13273986.00 14363117.00 21922346.00 F 12223906.00 11538499.00 12610844.00 12297502.00 12429429.00 6962799.00 10796810.00 13904208.00 13179710.00 13523549.00 16222937.00 21599704.00 G 13426931.00 13743966.00 11560183.00 11882716.00 12173603.00 12575191.00 15920162.00 14891277.00 13244419.00 15991987.00 18238122.00 19894716.00 H 12008406.00 12997294.00 12006227.00 12282594.00 11762770.00 11280302.00 11782433.00 13219000.00 138419.00 16756339.00 17593754.00 19249680.00 Load time: 07/30/2010 09:41:16 AM Read time: 07/30/2010 09:41:16 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #985 Comment: PR 9 phusion Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 35547396 cps Min counts: 3676820 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19089694.00 16257060.00 15787532.00 15507656.00 16308054.00 16126949.00 22850238.00 23417342.00 23700992.00 26164658.00 28337574.00 23824446.00 B 19715964.00 20063518.00 15268850.00 18422290.00 16576836.00 16370439.00 17842604.00 21228950.00 22072144.00 25044052.00 13086263.00 32137900.00 C 19219274.00 16524693.00 16739061.00 15663460.00 16808728.00 16053572.00 21310946.00 22961570.00 26233432.00 25041052.00 24282230.00 28360024.00 D 16091581.00 15447585.00 14963633.00 14970759.00 18618594.00 16758706.00 22066212.00 22470384.00 23150762.00 22662592.00 33208876.00 27793258.00 E 15081006.00 15517250.00 15621135.00 16778798.00 16827908.00 16004482.00 22523794.00 23064096.00 24453968.00 26232944.00 30361298.00 29387852.00 F 16570143.00 16098683.00 15447902.00 15806231.00 15797342.00 16754943.00 23517276.00 24855536.00 25547790.00 25865832.00 30745882.00 28958228.00 G 16782098.00 15968927.00 16115251.00 20259882.00 15773993.00 15262886.00 24771560.00 23286754.00 20410586.00 24205132.00 32885626.00 23710818.00 H 17591144.00 20519298.00 18251050.00 22032508.00 17062936.00 15556859.00 24926664.00 24307626.00 27246926.00 28577980.00 35547396.00 28605816.00 Load time: 07/30/2010 09:43:40 AM Read time: 07/30/2010 09:43:40 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #986 Comment: T12:18 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 31198518 cps Min counts: 3676820 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19480924.00 21672810.00 20527284.00 21494808.00 23386538.00 22637200.00 19468092.00 19825344.00 16200400.00 15834405.00 16782158.00 18140304.00 B 13620476.00 18531246.00 20758254.00 19788778.00 27319118.00 30771072.00 25828494.00 19669514.00 18714438.00 17105380.00 15401391.00 16889258.00 C 18285142.00 23976078.00 26508488.00 26079366.00 25701172.00 28657252.00 24565556.00 24124202.00 26637100.00 22870404.00 16966562.00 18504936.00 D 19029586.00 23283392.00 29145538.00 28715508.00 30104482.00 22819986.00 22443734.00 29498314.00 30105596.00 26461424.00 23530852.00 19807914.00 E 18140876.00 24424856.00 26007644.00 28699328.00 27772138.00 28855506.00 26757362.00 27421992.00 23366356.00 24341726.00 20575826.00 17348670.00 F 24909830.00 21450378.00 26576376.00 24503038.00 26346614.00 30454676.00 26189354.00 24893504.00 23202930.00 23241902.00 20844406.00 21019554.00 G 19394106.00 19997650.00 22057726.00 24065100.00 24602906.00 31198518.00 30424964.00 28234082.00 24836028.00 19098818.00 22374518.00 16365316.00 H 23089800.00 22808068.00 21930856.00 22893530.00 23166324.00 23269336.00 21901700.00 22152586.00 24157616.00 20091474.00 21617468.00 15593767.00 Load time: 07/30/2010 09:46:17 AM Read time: 07/30/2010 09:46:17 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #987 Comment: Tso7 CON 1 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 25796616 cps Min counts: 152108 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6727522.00 3181491.00 7391110.00 17204694.00 16746784.00 19261956.00 12689730.00 17920436.00 6450324.00 4532291.00 6905798.00 7045923.00 B 15139477.00 5621784.00 6166125.00 12889111.00 11787453.00 2527186.00 19973630.00 11800698.00 9481149.00 10591130.00 6121601.00 11708819.00 C 1886165.00 3964790.00 7508983.00 11729960.00 10429911.00 4556169.00 15079021.00 15376575.00 7271684.00 6650323.00 9615366.00 10418354.00 D 1100443.00 2276558.00 1474947.00 9932085.00 18740708.00 2747741.00 25796616.00 19469630.00 6914864.00 12749104.00 6586949.00 9604035.00 E 2351962.00 8949098.00 2903037.00 16432357.00 19811648.00 7858132.00 22310624.00 19705196.00 2572966.00 2938404.00 5563526.00 1803222.00 F 1447748.00 7539816.00 10638916.00 13278805.00 11528778.00 6930304.00 11974250.00 12742271.00 4981661.00 5735966.00 3763624.00 7728323.00 G 1180693.00 8082256.00 10696254.00 9232366.00 13261840.00 9055364.00 3789118.00 12997533.00 7176353.00 8257107.00 5053474.00 1236438.00 H 304217.00 6453287.00 8013137.00 11914768.00 9564076.00 7698164.00 13301310.00 12809949.00 3721703.00 5075646.00 10220624.00 3723588.00 Load time: 07/30/2010 09:49:09 AM Read time: 07/30/2010 09:49:09 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #988 Comment: T12:20 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 33621928 cps Min counts: 3676820 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18558550.00 22543072.00 21470882.00 25110072.00 21151450.00 24700982.00 22718900.00 23336032.00 19274142.00 20004268.00 21985698.00 22070966.00 B 20872962.00 20825458.00 21660348.00 24269856.00 29724720.00 29616164.00 25417086.00 25252414.00 20128474.00 17518972.00 18129428.00 24601800.00 C 21263338.00 25757962.00 27835550.00 30780708.00 27281538.00 29397160.00 23526064.00 28473834.00 28181712.00 24430928.00 22198570.00 21580446.00 D 23472114.00 28813330.00 26229052.00 32819696.00 32495930.00 27248804.00 26384822.00 27250218.00 33470548.00 31650886.00 33237272.00 18371636.00 E 19863796.00 28600078.00 26838340.00 31539272.00 23169376.00 26627030.00 26309268.00 29318512.00 31487902.00 27331568.00 33621928.00 24888194.00 F 26253850.00 21590774.00 17883332.00 23304466.00 26510552.00 28360154.00 20797364.00 24167012.00 31798618.00 27752920.00 27657846.00 21843928.00 G 26799294.00 20349114.00 20980368.00 21405684.00 23901374.00 29903766.00 25153308.00 26216860.00 23226518.00 19923652.00 21511710.00 19902588.00 H 20808368.00 23057858.00 18127810.00 24084004.00 23573514.00 28837058.00 25400630.00 21477544.00 20622488.00 19819486.00 29510388.00 22411148.00