Load time: 08/05/2010 09:02:30 AM Read time: 08/05/2010 09:02:30 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #994 Comment: Standard Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 93447600 cps Min counts: 60549 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 93447600.00 88121312.00 90690112.00 125229.00 B 45713528.00 49318912.00 47634600.00 126306.00 C 32545058.00 31155220.00 32472544.00 123523.00 D 18725222.00 18248638.00 18286518.00 125318.00 E 10858566.00 10644663.00 10379832.00 123792.00 F 6311096.00 6347812.00 6257863.00 125049.00 G 3533793.00 3490165.00 3446358.00 125677.00 H 2132395.00 2097295.00 2085266.00 121099.00 Load time: 08/05/2010 09:05:16 AM Read time: 08/05/2010 09:05:16 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #995 Comment: T11:11 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 25475082 cps Min counts: 718 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22769176.00 22112774.00 1705.00 21050940.00 21203616.00 20789934.00 20244790.00 19963114.00 19737862.00 20256958.00 19449880.00 20039470.00 B 1436.00 22699110.00 20797178.00 21128758.00 21347200.00 20317818.00 20136804.00 19697346.00 20478512.00 20078536.00 20292218.00 24471070.00 C 23912822.00 23932198.00 21646986.00 22083984.00 21575372.00 20735816.00 20426172.00 19846304.00 20966746.00 20655220.00 20634252.00 23716706.00 D 22307174.00 25373996.00 22027662.00 22739818.00 20826602.00 22024072.00 20168172.00 21324510.00 21664764.00 22461442.00 24221312.00 24153024.00 E 23533462.00 25302880.00 22755440.00 22682136.00 22539370.00 21995838.00 21315248.00 21573904.00 18958950.00 22710116.00 22415966.00 24648168.00 F 22856392.00 23023092.00 22413194.00 22285610.00 21549022.00 20718558.00 20324824.00 19898372.00 20684478.00 21715676.00 22965972.00 25475082.00 G 22312032.00 22399406.00 19861430.00 22282328.00 20660492.00 20827820.00 20471958.00 21128016.00 20792320.00 19813504.00 23551564.00 22906016.00 H 22525068.00 22629762.00 20186618.00 22495784.00 21444986.00 20596134.00 20833318.00 21395668.00 19644496.00 22357566.00 23634296.00 22489220.00 Load time: 08/05/2010 09:13:33 AM Read time: 08/05/2010 09:13:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #996 Comment: T11:11 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 26377784 cps Min counts: 65397 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23486446.00 22973504.00 130794.00 21756408.00 21963388.00 21512812.00 20922510.00 20642814.00 20070502.00 20829562.00 19937008.00 20499016.00 B 19911724.00 23575484.00 21434772.00 21973024.00 22137630.00 20894962.00 20775430.00 20360536.00 21076186.00 20562480.00 20750864.00 24880142.00 C 24725600.00 24837892.00 22349844.00 22872316.00 22371616.00 21041800.00 21078466.00 20408192.00 21509552.00 21268470.00 21202602.00 24241126.00 D 23235606.00 26377784.00 22716624.00 23500222.00 21619824.00 22835228.00 20808440.00 22076306.00 22236568.00 22947740.00 24820186.00 24755500.00 E 24452696.00 25980962.00 23409080.00 23428798.00 23298472.00 22615092.00 21944540.00 22094450.00 19438230.00 23231804.00 22902676.00 25149164.00 F 23694194.00 23832688.00 23204678.00 22960684.00 22124776.00 21357118.00 20858184.00 20420830.00 21175456.00 22172486.00 23590094.00 26073224.00 G 23097834.00 23191286.00 20519860.00 22966116.00 21268054.00 21427326.00 21094720.00 21724684.00 21342182.00 20403128.00 24089632.00 23316908.00 H 23330400.00 23541102.00 20928226.00 23272870.00 22162516.00 21226186.00 21341638.00 22137510.00 20062160.00 23076990.00 24044848.00 23102708.00 Load time: 08/05/2010 09:15:52 AM Read time: 08/05/2010 09:15:52 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #997 Comment: T12:21 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 23603662 cps Min counts: 3676976 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20397978.00 19186032.00 18731354.00 18361682.00 18999388.00 18754954.00 18667130.00 18631414.00 18176110.00 19441334.00 19172838.00 18902616.00 B 17588358.00 17161610.00 19130562.00 18389094.00 19119660.00 19008278.00 18817516.00 18631610.00 18307386.00 19592004.00 18727864.00 22524322.00 C 19508658.00 18835764.00 19531604.00 19255620.00 19126554.00 19098814.00 18614672.00 18328276.00 18536754.00 18907678.00 18086090.00 20988624.00 D 18438890.00 19670916.00 19092468.00 19772380.00 19606378.00 19452586.00 19227780.00 19085702.00 18969084.00 19180206.00 21093536.00 21727826.00 E 19809886.00 20155136.00 19789638.00 23603662.00 19787756.00 19453574.00 19321206.00 18585352.00 18167220.00 18929796.00 18752218.00 23019500.00 F 19415228.00 19997632.00 19657582.00 18570504.00 19172028.00 19062024.00 18752516.00 18373482.00 18794354.00 19510262.00 19464880.00 22417540.00 G 18005558.00 19099470.00 18672096.00 18605686.00 18552112.00 18675880.00 18752296.00 17736172.00 18564540.00 18914388.00 20315448.00 21559888.00 H 19637898.00 19138100.00 19386412.00 19001304.00 19564722.00 19136498.00 18693016.00 18508426.00 17753882.00 18554122.00 17272240.00 21911950.00 Load time: 08/05/2010 09:20:50 AM Read time: 08/05/2010 09:20:50 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #998 Comment: T12:21 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 28636174 cps Min counts: 3676976 counts Well list: r0(a4) = cps(f2(a4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28636174.00 B C D E F G H