Load time: 08/31/2010 03:04:52 PM Read time: 08/31/2010 03:04:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1017 Comment: Standard Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 96428008 cps Min counts: 83273 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85938880.00 93701296.00 96428008.00 172114.00 B 52902444.00 54429104.00 54650084.00 170228.00 C 36820828.00 32157594.00 32073538.00 166547.00 D 22054780.00 16429529.00 18304154.00 167624.00 E 11557421.00 10398571.00 10078185.00 168253.00 F 6642420.00 6187220.00 6380163.00 203179.00 G 3851334.00 4048497.00 3660275.00 167535.00 H 2159282.00 2055942.00 2099846.00 168343.00 Load time: 08/31/2010 03:07:10 PM Read time: 08/31/2010 03:07:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1018 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 23245316 cps Min counts: 785511 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16244504.00 13774290.00 15424743.00 17481850.00 18107420.00 18736678.00 16858662.00 17377312.00 16646373.00 17710536.00 14377564.00 18815750.00 B 13819395.00 13174961.00 12447725.00 15001712.00 16605994.00 18220432.00 14685313.00 17135950.00 15200369.00 12691580.00 13983988.00 18437118.00 C 15199654.00 14003745.00 16435631.00 15234385.00 17818310.00 13338871.00 18083334.00 16125379.00 20777816.00 15823966.00 15415562.00 19545398.00 D 11014908.00 13125244.00 17994926.00 19079980.00 19944202.00 21544354.00 18152322.00 18964526.00 23245316.00 20252554.00 20734144.00 18834510.00 E 13255321.00 12820255.00 16649245.00 17311338.00 17952104.00 19231472.00 18755886.00 18136272.00 20433298.00 18105306.00 15806798.00 19619768.00 F 13107803.00 11413919.00 11883487.00 14053998.00 16568704.00 17666900.00 18300858.00 17950448.00 17231094.00 16144443.00 17278050.00 18106276.00 G 9860270.00 9455760.00 1571023.00 11464718.00 14461188.00 16658083.00 14372309.00 16654485.00 14853948.00 13567847.00 15484130.00 15967307.00 H 12898940.00 10773481.00 10208289.00 12508299.00 14424032.00 15448518.00 16904898.00 14740731.00 13588251.00 15978790.00 18518474.00 16184636.00