Load time: 09/08/2010 10:31:29 AM Read time: 09/08/2010 10:31:29 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1028 Comment: CowpeaSTD Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 84510480 cps Min counts: 1066 counts Well list: r0(a1:h3) = cps(f2(a1:h3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 84510480.00 74517960.00 231438.00 B 40175508.00 52740480.00 193065.00 C 29875398.00 31667666.00 167413.00 D 16280474.00 16867738.00 193122.00 E 9547697.00 9583486.00 2342.00 F 5672429.00 5565226.00 2132.00 G 3398487.00 3166655.00 2285.00 H 2234227.00 2001087.00 2209.00 Load time: 09/08/2010 10:33:27 AM Read time: 09/08/2010 10:33:27 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1029 Comment: CowpeaPLATE1 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21071572 cps Min counts: 780031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4305260.00 2690890.00 5938393.00 5360121.00 7989598.00 15045861.00 16027313.00 10111555.00 13779019.00 16203399.00 10796957.00 6929580.00 B 6925027.00 6321169.00 8821334.00 3342082.00 15738439.00 16227028.00 15776254.00 14405282.00 17399614.00 12076027.00 6062228.00 12666676.00 C 5794512.00 6502301.00 5997425.00 6347589.00 14507223.00 14372979.00 18693286.00 17109748.00 15787813.00 19094790.00 7942543.00 7105951.00 D 6614086.00 6924702.00 10946277.00 3691238.00 8591574.00 13569568.00 18939196.00 16154005.00 16354614.00 12959575.00 3551510.00 2526985.00 E 6436244.00 5409463.00 8358864.00 8292483.00 14719550.00 13703205.00 19994816.00 16483732.00 11828339.00 10418147.00 5176685.00 5465748.00 F 5977066.00 3676755.00 16562309.00 2545861.00 12581926.00 15146921.00 20067550.00 14622669.00 18379372.00 6907360.00 8091001.00 8559511.00 G 6702604.00 2772527.00 11032230.00 7620615.00 12182931.00 10034252.00 21071572.00 15958127.00 13415699.00 4771871.00 4491324.00 6982948.00 H 4590130.00 4799379.00 7976086.00 11371718.00 10011533.00 9353704.00 15927789.00 8618201.00 11228921.00 9819784.00 5253318.00 7425309.00 Load time: 09/08/2010 10:35:40 AM Read time: 09/08/2010 10:35:40 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1030 Comment: CowpeaPLATE1 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18684376 cps Min counts: 780031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7324681.00 3745705.00 6272549.00 13720294.00 8719364.00 13028277.00 9517502.00 6973977.00 10672412.00 11104032.00 11086217.00 5459218.00 B 5968701.00 2859144.00 10264642.00 7735140.00 4077959.00 11706773.00 11057048.00 9534266.00 8865870.00 12711550.00 8001925.00 9417317.00 C 5216807.00 8759656.00 14853021.00 9399716.00 10864088.00 13791712.00 8470309.00 7320865.00 9954169.00 5705643.00 8136381.00 8667674.00 D 5417206.00 6205354.00 13413162.00 8950141.00 10847906.00 16099026.00 8748623.00 12698205.00 10970493.00 7175132.00 7508896.00 10342490.00 E 4505587.00 8680995.00 13121000.00 13675145.00 7038123.00 17609806.00 8754632.00 10805558.00 6677130.00 8133370.00 10314537.00 7405035.00 F 3779157.00 7179862.00 9785477.00 11762362.00 12242647.00 11413266.00 6844538.00 11847166.00 8755439.00 7733537.00 10474906.00 12632337.00 G 6300404.00 5125441.00 8728955.00 10877282.00 9159880.00 18684376.00 7226288.00 8730167.00 7921492.00 6001360.00 8251431.00 8538093.00 H 4974758.00 9462809.00 9453382.00 14377880.00 7528384.00 13330205.00 9396353.00 13731162.00 7664110.00 6495121.00 5605685.00 12017645.00 Load time: 09/08/2010 10:37:53 AM Read time: 09/08/2010 10:37:53 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1031 Comment: CowpeaPLATE3 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 17488440 cps Min counts: 667740 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8020429.00 2655605.00 11216199.00 7095524.00 6359823.00 6478669.00 8584172.00 6465608.00 9387408.00 9762453.00 6594560.00 4166039.00 B 12588730.00 2542205.00 6389167.00 10987894.00 13195780.00 12299556.00 17339546.00 8432328.00 13283216.00 10288701.00 8358829.00 8778585.00 C 11338542.00 8167067.00 10733666.00 13158384.00 8305437.00 11502889.00 12431359.00 10694830.00 11170773.00 11317354.00 11804782.00 7207526.00 D 6275728.00 2235317.00 1335480.00 9864528.00 10401310.00 17488440.00 15107610.00 10454128.00 12466822.00 9722783.00 10748086.00 6343644.00 E 8753169.00 6238372.00 1883208.00 9770181.00 12932030.00 9721923.00 15444459.00 12837471.00 9886345.00 8114992.00 12863404.00 4007670.00 F 4602760.00 5570914.00 3251478.00 5356407.00 10866024.00 11452359.00 7451332.00 6283251.00 7157921.00 9300407.00 6289137.00 6064306.00 G 5177798.00 2456935.00 5328154.00 9217312.00 4574694.00 12009947.00 5642414.00 6636054.00 11214441.00 8560733.00 10357982.00 10344589.00 H 4316378.00 4233247.00 3263568.00 7201005.00 3034159.00 12124304.00 6194843.00 4357631.00 6743368.00 4892504.00 8493449.00 7715500.00 Load time: 09/08/2010 10:40:06 AM Read time: 09/08/2010 10:40:06 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1032 Comment: CowpeaPLATE4 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21531418 cps Min counts: 780031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7025008.00 7348300.00 8805778.00 9382441.00 9453600.00 9319130.00 17692406.00 7205789.00 10776969.00 9182319.00 12007784.00 7575594.00 B 7579643.00 5392686.00 10367040.00 14462519.00 7626046.00 6887119.00 11303725.00 18948720.00 11544993.00 13488404.00 10872248.00 7431194.00 C 6713391.00 11860209.00 16206362.00 11667025.00 14922810.00 7108217.00 11613162.00 6135913.00 12757247.00 7463383.00 16337453.00 11807283.00 D 10133992.00 5732112.00 11784807.00 15418204.00 13256466.00 9083148.00 4417733.00 12543122.00 12135263.00 7386901.00 14249965.00 8822720.00 E 8275100.00 8002910.00 15985959.00 11722290.00 13225695.00 12095184.00 7304071.00 11228339.00 10038720.00 6957443.00 8946282.00 12941944.00 F 6364815.00 4870172.00 10822154.00 10991362.00 3667887.00 6926577.00 8652300.00 21531418.00 8475122.00 9805576.00 6329550.00 8545614.00 G 8777153.00 5678962.00 4653577.00 7295538.00 4698400.00 4551944.00 8569657.00 10901224.00 10930601.00 7325203.00 5045816.00 10307600.00 H 7766245.00 4258954.00 8390063.00 5299934.00 5531017.00 3281111.00 6917449.00 6879114.00 7984813.00 8616888.00 8519517.00 7272588.00